##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545583_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1528056
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.506171239797496	33.0	31.0	34.0	31.0	34.0
2	32.9356574628155	34.0	31.0	34.0	31.0	34.0
3	33.0311879931102	34.0	33.0	34.0	31.0	34.0
4	36.42036352070866	37.0	37.0	37.0	35.0	37.0
5	36.39696647243295	37.0	37.0	37.0	35.0	37.0
6	36.41470665996534	37.0	37.0	37.0	35.0	37.0
7	36.47300295277137	37.0	37.0	37.0	35.0	37.0
8	36.505368913181194	37.0	37.0	37.0	35.0	37.0
9	38.332246985712565	39.0	39.0	39.0	37.0	39.0
10	38.07132919212385	39.0	39.0	39.0	35.0	39.0
11	37.88342508389745	39.0	38.0	39.0	35.0	39.0
12	37.53901820352134	39.0	37.0	39.0	35.0	39.0
13	37.49369329396305	39.0	37.0	39.0	35.0	39.0
14	38.866164590826514	41.0	38.0	41.0	35.0	41.0
15	38.88674367955101	41.0	38.0	41.0	35.0	41.0
16	38.917147015554406	41.0	38.0	41.0	35.0	41.0
17	38.844773359091555	41.0	38.0	41.0	35.0	41.0
18	38.83206440078112	40.0	38.0	41.0	35.0	41.0
19	38.81173072191071	40.0	38.0	41.0	35.0	41.0
20	38.7314574858513	40.0	38.0	41.0	35.0	41.0
21	38.68653373960117	40.0	38.0	41.0	35.0	41.0
22	38.78289604569466	40.0	38.0	41.0	35.0	41.0
23	38.781100299989006	40.0	38.0	41.0	35.0	41.0
24	38.7131865586078	40.0	38.0	41.0	35.0	41.0
25	38.62786965922715	40.0	37.0	41.0	35.0	41.0
26	38.50400705209757	40.0	37.0	41.0	35.0	41.0
27	38.42072607286644	40.0	37.0	41.0	35.0	41.0
28	38.38025635186145	40.0	37.0	41.0	35.0	41.0
29	38.310044919819696	40.0	36.0	41.0	35.0	41.0
30	38.255885255514194	40.0	36.0	41.0	34.0	41.0
31	38.14966794410676	40.0	36.0	41.0	34.0	41.0
32	38.08780895464564	40.0	36.0	41.0	34.0	41.0
33	38.01669048778317	40.0	36.0	41.0	34.0	41.0
34	37.91286706769909	40.0	35.0	41.0	34.0	41.0
35	37.792179736868285	40.0	35.0	41.0	34.0	41.0
36	37.71246472642364	40.0	35.0	41.0	33.0	41.0
37	37.65479275628642	40.0	35.0	41.0	33.0	41.0
38	37.54692759951206	40.0	35.0	41.0	33.0	41.0
39	37.460849602370594	40.0	35.0	41.0	33.0	41.0
40	37.31725277084086	39.0	35.0	41.0	33.0	41.0
41	37.177649248456866	39.0	35.0	41.0	33.0	41.0
42	37.07510981272937	39.0	35.0	41.0	33.0	41.0
43	36.98000335066254	39.0	35.0	41.0	33.0	41.0
44	36.8541584863382	39.0	35.0	41.0	32.0	41.0
45	36.77153062453208	39.0	35.0	41.0	32.0	41.0
46	36.73263807085604	38.0	35.0	41.0	32.0	41.0
47	36.69117034977776	38.0	35.0	41.0	32.0	41.0
48	36.573101378483514	38.0	35.0	40.0	32.0	41.0
49	36.47356903150146	38.0	35.0	40.0	32.0	41.0
50	36.38034797154031	38.0	35.0	40.0	32.0	41.0
51	36.277544802022966	37.0	35.0	40.0	32.0	41.0
52	35.93068644081107	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	4.0
12	9.0
13	32.0
14	27.0
15	60.0
16	79.0
17	157.0
18	277.0
19	505.0
20	878.0
21	1308.0
22	1953.0
23	2654.0
24	3661.0
25	4738.0
26	5918.0
27	7487.0
28	9415.0
29	12071.0
30	15451.0
31	19779.0
32	26187.0
33	36535.0
34	82296.0
35	160779.0
36	101902.0
37	144365.0
38	236721.0
39	651766.0
40	1036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.34712863926453	13.71703654839875	45.86081923699131	12.075015575345406
2	25.72569329919846	12.753328412047725	49.42109451486071	12.099883773893104
3	26.107420146905614	14.19038307496584	45.139183380713796	14.563013397414753
4	23.18272366981315	14.275393048422307	46.991340631495184	15.55054265026936
5	22.42790840126278	17.46100928238232	44.42985073845461	15.681231577900286
6	26.144591559471642	19.833042768066093	42.779060453281815	11.24330521918045
7	88.29323009104378	2.720319150607046	5.4095530530294695	3.5768977053197
8	89.16296261393562	2.7513389561639103	5.402288921348433	2.6834095085520424
9	82.2698906322805	4.33753736774045	7.139201704649569	6.253370295329491
10	46.309428450266225	22.728028292156832	12.993306528033004	17.969236729543944
11	35.25453255639845	23.626097472867485	21.946185218342784	19.173184752391272
12	29.177530142874346	23.17480511185454	26.376520232242797	21.271144513028318
13	23.86725355615239	24.742156046637035	30.804957409937856	20.585632987272717
14	20.30638929463318	29.649633259514047	29.172621945792564	20.871355500060208
15	19.448109231598842	22.009010140989595	36.182247247483076	22.360633379928487
16	23.929685823032663	20.905909207515954	32.37937614851812	22.785028820933263
17	23.743632432319234	21.450195542571738	27.41915217766888	27.387019847440147
18	24.310300146067945	21.598619422324834	29.44525593302863	24.645824498578587
19	27.832226044071685	24.334448475710314	25.489314527739822	22.344010952478182
20	29.077730135544773	21.774790976246944	24.766893359929217	24.38058552827907
21	27.22014114665955	24.510358259121393	25.323941007397632	22.945559586821425
22	29.67351981864539	21.24614542922511	24.02582104320784	25.05451370892166
23	29.416853832582053	23.190053244121945	22.38595967687048	25.00713324642552
24	28.50111514237698	19.529650745784185	27.29585826697451	24.673375844864324
25	29.97076023391813	22.144149167308004	23.23906977231201	24.646020826461857
26	25.028009444680038	26.32547498259226	22.678095567178165	25.968420005549536
27	24.21501568005361	24.909689173695206	24.21972754925212	26.655567596999063
28	24.662774139167677	23.771772762254788	26.30008324302251	25.26536985555503
29	24.016200976927546	23.512489071081163	24.67547000895255	27.795839943038736
30	24.866758809886548	27.332048040124185	23.872816179511748	23.928376970477522
31	27.626997963425424	26.255451370892168	22.324116393640022	23.793434272042386
32	29.790923892841626	24.040938290219728	21.134565748899256	25.03357206803939
33	27.65913029365416	26.48175197767621	21.772696812158717	24.086420916510914
34	25.8311868151429	25.91240111618946	23.229187935520688	25.02722413314695
35	26.46447512394834	24.158276921788207	25.426293277209737	23.95095467705372
36	26.581682870261297	26.541370211562924	21.47944839717916	25.397498520996614
37	28.444703597250363	27.04279162543781	22.527053982314786	21.985450794997043
38	24.768725753506416	26.076204013465475	24.509507504960553	24.645562728067556
39	26.1667766102813	27.33211348275194	22.175561628631414	24.325548278335347
40	26.647583596412698	23.239462428078554	25.588852764558368	24.524101210950384
41	23.952983398514192	24.057887930808818	24.991754228902604	26.997374441774387
42	24.942083274434967	24.984359211966055	22.780382394362512	27.29317511923647
43	23.926217363761538	22.559448083054548	26.039883355060283	27.474451198123628
44	27.137159894663547	23.21387436062553	23.372638175564248	26.27632756914668
45	23.766406466778704	25.11478636908595	24.042378028030388	27.076429136104956
46	24.822454150894995	24.597789609804877	23.353528928259173	27.226227311040958
47	25.336047893532697	23.96607192406561	26.237323763003452	24.46055641939824
48	23.94009120084604	22.008617485223056	28.4376357934526	25.613655520478307
49	23.927002675294624	23.228926165009657	25.621443193181403	27.222627966514317
50	23.2163611804803	23.716342856544525	27.165365667226855	25.901930295748322
51	24.3126560806672	23.816470077012887	25.57216489448031	26.298708947839604
52	24.44367222143691	22.417699351332672	26.264286125639373	26.874342301591042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	93.0
2	80.0
3	87.5
4	95.0
5	96.5
6	98.0
7	197.0
8	296.0
9	382.0
10	468.0
11	596.5
12	725.0
13	934.0
14	1099.0
15	1055.0
16	983.5
17	912.0
18	883.5
19	855.0
20	1527.0
21	2199.0
22	2239.5
23	2280.0
24	2744.0
25	3208.0
26	4341.0
27	5474.0
28	6609.0
29	7744.0
30	11361.5
31	14979.0
32	17287.0
33	19595.0
34	21970.0
35	24345.0
36	24916.0
37	25487.0
38	30202.5
39	45188.5
40	55459.0
41	61785.5
42	68112.0
43	77209.0
44	86306.0
45	87156.0
46	88006.0
47	98869.0
48	109732.0
49	122840.0
50	135948.0
51	139823.0
52	143698.0
53	130713.0
54	117728.0
55	112021.5
56	106315.0
57	101466.5
58	96618.0
59	93968.0
60	91318.0
61	85343.5
62	79369.0
63	70154.5
64	54004.5
65	47069.0
66	38296.5
67	29524.0
68	26340.5
69	23157.0
70	18646.5
71	14136.0
72	12320.0
73	10504.0
74	9153.0
75	7802.0
76	5878.5
77	3955.0
78	3884.5
79	3814.0
80	2760.0
81	1706.0
82	1026.0
83	346.0
84	290.5
85	235.0
86	151.5
87	68.0
88	65.5
89	41.0
90	19.0
91	18.0
92	17.0
93	17.0
94	17.0
95	10.5
96	4.0
97	5.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1528056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.870143351467544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3231699608924	11.86361958169871
2	11.903391711950967	4.016238490985669
3	4.813381373999781	2.436073013539804
4	2.4417664070385694	1.6477179727015407
5	1.409229541176246	1.1886952187383055
6	0.8900915061397775	0.9009582782701021
7	0.6687729464754594	0.7897606833637031
8	0.45966754809010696	0.6203725944238164
9	0.3510914461814133	0.5330666723899046
>10	3.44645619492844	13.975011252477076
>50	1.6812907664532237	20.658932381756443
>100	1.6031363884403682	38.957137805883015
>500	0.0038882764696589033	0.45149576360529275
>1k	0.0046659317635906845	1.9609202901665874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT	4964	0.32485720418623404	Illumina Single End Adapter 1 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	3575	0.23395739423162504	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	3526	0.2307507054715272	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3058	0.2001235556812054	No Hit
TGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	2802	0.18337024297538834	No Hit
TTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	2590	0.16949640589088358	No Hit
ATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	2228	0.14580617464281415	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1775	0.11616066426884879	No Hit
GGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1575	0.10307213871742921	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1529	0.1000617778406027	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33997445119812364	0.0
2	0.0	0.0	0.0	0.7133900851801243	0.0
3	0.0	0.0	0.0	2.325961875742774	0.0
4	0.0	0.0	0.0	2.6619443266477143	0.0
5	0.0	0.0	0.0	3.296017946986236	0.0
6	0.0	0.0	0.0	5.128935065207035	0.0
7	0.0	0.0	0.0	5.352814294764066	0.0
8	0.0	0.0	0.0	5.792392425408493	0.0
9	6.54426277570979E-5	0.0	0.0	6.079554676006638	0.0
10	6.54426277570979E-5	0.0	0.0	7.172446559550173	0.0
11	1.308852555141958E-4	0.0	0.0	8.098917840707408	0.0
12	1.308852555141958E-4	0.0	0.0	8.47999026213699	0.0
13	1.308852555141958E-4	0.0	0.0	8.684433031250164	0.0
14	1.308852555141958E-4	0.0	0.0	8.78776694047862	0.0
15	1.308852555141958E-4	0.0	0.0	8.964920133817085	0.0
16	1.308852555141958E-4	0.0	0.0	9.360717146492014	0.0
17	1.308852555141958E-4	0.0	0.0	9.8792190862115	0.0
18	1.308852555141958E-4	0.0	0.0	10.457666472956488	0.0
19	1.308852555141958E-4	0.0	0.0	10.820153188103054	0.0
20	1.308852555141958E-4	0.0	0.0	11.223999643992105	0.0
21	1.308852555141958E-4	0.0	0.0	11.833924934688257	0.0
22	1.308852555141958E-4	0.0	0.0	12.360476317621867	0.0
23	1.308852555141958E-4	0.0	0.0	12.945926065536865	0.0
24	1.308852555141958E-4	0.0	0.0	13.397022098666541	0.0
25	1.9632788327129372E-4	0.0	0.0	13.797334652656708	0.0
26	1.9632788327129372E-4	0.0	0.0	14.143984251886057	0.0
27	1.9632788327129372E-4	0.0	0.0	14.47381509578183	0.0
28	1.9632788327129372E-4	0.0	0.0	14.819744826105849	0.0
29	1.9632788327129372E-4	0.0	0.0	15.17850131147026	0.0
30	1.9632788327129372E-4	0.0	0.0	15.564220159470596	0.0
31	2.617705110283916E-4	0.0	0.0	15.937374022941567	0.0
32	2.617705110283916E-4	0.0	0.0	16.29586873779495	0.0
33	2.617705110283916E-4	0.0	0.0	16.659533420241143	0.0
34	2.617705110283916E-4	0.0	0.0	17.021627479621166	0.0
35	2.617705110283916E-4	0.0	0.0	17.432018198285927	0.0
36	2.617705110283916E-4	0.0	0.0	17.805499274895684	0.0
37	2.617705110283916E-4	0.0	0.0	18.183692220703954	0.0
38	2.617705110283916E-4	0.0	0.0	18.549385624610615	0.0
39	2.617705110283916E-4	0.0	0.0	18.941256079620118	0.0
40	2.617705110283916E-4	0.0	0.0	19.350665158868523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACG	35	1.019871E-7	46.000004	27
TCGTATC	35	1.019871E-7	46.000004	12
TCGTAAT	35	1.019871E-7	46.000004	32
CGTAATC	35	1.019871E-7	46.000004	33
AGCGTAC	20	6.3121103E-4	46.0	20
CATGCGT	25	3.417689E-5	46.0	18
TAACGTA	20	6.3121103E-4	46.0	46
CGATTAG	25	3.417689E-5	46.0	1
TTATCGC	20	6.3121103E-4	46.0	26
GTATCGA	20	6.3121103E-4	46.0	14
TCCCGTA	20	6.3121103E-4	46.0	20
TTACGAC	20	6.3121103E-4	46.0	37
TTACCGG	45	3.110472E-10	46.0	2
TTCGATC	25	3.417689E-5	46.0	21
AATACGC	25	3.417689E-5	46.0	35
AATACCC	20	6.3121103E-4	46.0	39
ATCGCAA	20	6.3121103E-4	46.0	28
GCGTACA	25	3.417689E-5	46.0	32
ACGAATC	20	6.3121103E-4	46.0	34
ACGCGTT	20	6.3121103E-4	46.0	41
>>END_MODULE