FastQCFastQC Report
Thu 26 May 2016
SRR1545582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545582_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4201285
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110780.26368123086151024No Hit
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC83080.19774902202540412Illumina PCR Primer Index 5 (95% over 23bp)
ATGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT52460.12486655868383124Illumina PCR Primer Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA207.03543E-445.014
ATTACGC3400.043.0147061
ATACGTC2000.040.51
CGTTTTT63850.039.7141761
AGTCCGT403.4611548E-739.37541
GTACGAA700.038.57142619
TACGGGA8350.037.724556
ATCGGTT4000.037.68751
ATGCGAT4800.037.5000041
ATGCGTT13550.037.3616261
GCTACGA5600.036.96428310
AGGGCGA45700.036.8763668
TTACGGG13750.036.818185
TTTACGG7300.036.678084
TACGTCC800.036.56252
CGCGAAA250.002107744336.030
TAATCCG250.002107744336.03
CTACGAA5800.035.68965511
TTGGGCG70250.035.6156587
GGGCGAT200200.035.54829
Produced by FastQC (version 0.11.5)