##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545582_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4201285
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847004666429438	31.0	31.0	34.0	31.0	34.0
2	32.501778860515294	33.0	31.0	34.0	31.0	34.0
3	32.488434609887214	34.0	31.0	34.0	31.0	34.0
4	35.98288499828029	37.0	35.0	37.0	35.0	37.0
5	35.75736542510208	37.0	35.0	37.0	35.0	37.0
6	35.75659899292717	37.0	35.0	37.0	35.0	37.0
7	35.74361796450372	37.0	35.0	37.0	33.0	37.0
8	35.70929798859159	37.0	35.0	37.0	33.0	37.0
9	37.8730119475351	39.0	38.0	39.0	35.0	39.0
10	37.798922234506826	39.0	38.0	39.0	35.0	39.0
11	37.62513183466487	39.0	37.0	39.0	35.0	39.0
12	37.16041377816549	39.0	37.0	39.0	33.0	39.0
13	36.94033706354127	39.0	37.0	39.0	33.0	39.0
14	37.84983284875937	40.0	36.0	41.0	33.0	41.0
15	37.8566764692231	40.0	37.0	41.0	33.0	41.0
16	37.75671133950684	40.0	36.0	41.0	32.0	41.0
17	37.82856411788298	40.0	36.0	41.0	33.0	41.0
18	37.86524194383385	40.0	36.0	41.0	33.0	41.0
19	37.83147679817009	40.0	36.0	41.0	33.0	41.0
20	37.76654761578898	40.0	36.0	41.0	33.0	41.0
21	37.76568478453616	40.0	36.0	41.0	33.0	41.0
22	37.70745521905798	40.0	36.0	41.0	32.0	41.0
23	37.66256633387166	40.0	36.0	41.0	32.0	41.0
24	37.65109579569108	40.0	36.0	41.0	32.0	41.0
25	37.586988266685076	40.0	35.0	41.0	32.0	41.0
26	37.486046054956994	40.0	35.0	41.0	32.0	41.0
27	37.40528338353623	40.0	35.0	41.0	32.0	41.0
28	37.30452730533634	40.0	35.0	41.0	32.0	41.0
29	37.28541458149114	40.0	35.0	41.0	32.0	41.0
30	37.21264351263959	39.0	35.0	41.0	31.0	41.0
31	37.09780579037128	39.0	35.0	41.0	31.0	41.0
32	36.97325461138676	39.0	35.0	41.0	31.0	41.0
33	36.874130890905995	39.0	35.0	41.0	31.0	41.0
34	36.815602845319944	39.0	35.0	41.0	31.0	41.0
35	36.724891789059775	39.0	35.0	41.0	30.0	41.0
36	36.600173042295395	39.0	35.0	41.0	30.0	41.0
37	36.50530944699062	39.0	35.0	41.0	30.0	41.0
38	36.458469730094485	39.0	35.0	41.0	30.0	41.0
39	36.42341735921272	39.0	35.0	41.0	30.0	41.0
40	36.362433636375535	39.0	35.0	41.0	30.0	41.0
41	36.26881204203	39.0	35.0	41.0	30.0	41.0
42	36.16053778784348	38.0	35.0	41.0	30.0	41.0
43	36.05147948782337	38.0	35.0	40.0	29.0	41.0
44	35.989926177348124	38.0	35.0	40.0	29.0	41.0
45	35.96142394529293	38.0	35.0	40.0	29.0	41.0
46	35.88830322151437	38.0	35.0	40.0	29.0	41.0
47	35.83181383790912	38.0	35.0	40.0	29.0	41.0
48	35.750721743466585	38.0	35.0	40.0	29.0	41.0
49	35.72401777075347	38.0	35.0	40.0	29.0	41.0
50	35.629458368094525	38.0	34.0	40.0	28.0	41.0
51	35.43742093192916	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	6.0
9	19.0
10	38.0
11	47.0
12	55.0
13	61.0
14	94.0
15	188.0
16	300.0
17	703.0
18	1186.0
19	2431.0
20	3999.0
21	6384.0
22	9912.0
23	14515.0
24	21372.0
25	30066.0
26	40043.0
27	48845.0
28	56809.0
29	67213.0
30	81466.0
31	100477.0
32	124629.0
33	160664.0
34	252359.0
35	365903.0
36	307194.0
37	426734.0
38	667860.0
39	1409132.0
40	578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.3175052394684004	90.56105453450552	3.3568777171746262	3.7645625088514585
2	3.7995994082762774	3.038808364583693	89.96894997601925	3.192642251120788
3	29.337190883265478	16.456679325492082	40.921218151113294	13.284911640129152
4	27.980129888831627	15.983276545152256	41.91724674712617	14.119346818889936
5	25.875464292472422	14.67900892227021	44.54313382691248	14.902392958344887
6	23.633912005493556	14.913794232002827	46.06362101119063	15.38867275131299
7	23.603706961084526	17.35354778359478	43.316580522387795	15.7261647329329
8	26.019206028631714	19.93816177669451	42.36327694979036	11.67935524488341
9	81.65356551626466	4.502217773847763	9.358827120749961	4.4853895891376085
10	81.65485083730334	4.880030752495963	9.283445422055395	4.181672988145293
11	74.92364835996605	7.8558107817013125	10.904092438384923	6.316448419947706
12	38.53044961244	27.10085128716571	17.26038581053178	17.108313289862505
13	29.982683869339976	24.47284580788973	27.402949335738946	18.14152098703135
14	26.06714374292627	24.327937761899037	29.612987455028637	19.99193104014605
15	23.734595486856996	24.3674970872007	32.83052685071353	19.06738057522877
16	19.437886265749647	28.98660766884418	32.195221223982664	19.380284841423517
17	19.62780435033567	25.03260312023583	35.89544627417564	19.444146255252857
18	24.333293266226878	23.75804069469222	31.900954112848805	20.007711926232094
19	24.392465638489178	23.24141304386634	29.90361282321956	22.46250849442492
20	24.453732607999694	23.29051706799229	31.06720919909028	21.188541124917734
21	25.322942861529267	26.21916865911263	27.827938356955073	20.629950122403027
22	25.47518199788874	25.279170539489705	28.10778130976594	21.137866152855615
23	25.33996146417108	25.253154689577116	29.328907703238414	20.077976143013387
24	27.444198620184064	22.18019010850252	28.425350815286276	21.95026045602714
25	26.6227832675003	24.12930805693972	26.87277820952399	22.375130466035987
26	25.66143453729038	23.20380550236416	29.643406719610788	21.491353240734682
27	27.068408832059713	25.15063843562148	25.176820901224268	22.604131831094534
28	22.861933908316146	26.507723232296787	26.871112052621992	23.75923080676507
29	22.173549283135994	25.271982262569665	28.644450447898677	23.91001800639566
30	21.96439898745265	25.672573986292292	28.479881750464443	23.88314527579062
31	22.848247619478325	25.515026950087886	28.267280129769823	23.369445300663962
32	23.851178865513763	25.990619536641766	29.14094140245187	21.017260195392602
33	24.189075485238444	26.48063628151863	27.340254231741	21.990034001501922
34	25.31937252531071	26.410300658012964	26.539951467229667	21.730375349446657
35	24.94969991324083	25.756691107601604	27.81884590071847	21.474763078439096
36	23.37777608517394	24.967599198816554	29.503140110704223	22.15148460530528
37	22.206729607727162	27.654491423457344	28.929553696071558	21.209225272743932
38	23.118403060016163	28.714500444506857	26.62290227870759	21.544194216769395
39	23.174005096059897	28.62317124403605	27.857167509464364	20.345656150439687
40	23.489670422263664	25.866990694513703	29.586662175977114	21.056676707245522
41	22.332500651586358	27.635544839257513	26.538261508086215	23.49369300106991
42	24.39046625020678	25.318753667032823	28.75277444876984	21.538005633990554
43	21.73963442137346	25.74564686756552	29.349472839857327	23.16524587120369
44	21.52581888636453	26.251230278355315	28.078385541566448	24.14456529371371
45	21.314240762052563	24.860393903293872	28.767674651921972	25.057690682731593
46	23.014196846917073	25.27586202792717	27.90305823099361	23.806882894162143
47	21.797354856906875	25.770353594197964	27.518533020254516	24.913758528640642
48	20.895178498959247	26.654821084501528	27.673961656969237	24.77603875956999
49	21.24852277338957	25.961937835685987	30.20756744662645	22.581971944297994
50	21.29412786802133	25.554348252974986	30.366185583696414	22.78533829530727
51	21.85507529244029	25.513503606634636	28.395240979843074	24.236180121082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1578.0
1	2735.5
2	3893.0
3	11802.5
4	19712.0
5	13826.0
6	7940.0
7	8106.0
8	8272.0
9	8575.0
10	8878.0
11	8813.0
12	8748.0
13	9111.5
14	9475.0
15	10072.0
16	10669.0
17	11377.0
18	12085.0
19	12668.5
20	13252.0
21	16248.5
22	19245.0
23	22766.0
24	26287.0
25	31604.0
26	46952.5
27	56984.0
28	66218.0
29	75452.0
30	89326.0
31	103200.0
32	120325.0
33	137450.0
34	152880.5
35	168311.0
36	177542.0
37	186773.0
38	201469.0
39	216165.0
40	236007.0
41	255849.0
42	284684.0
43	313519.0
44	336833.0
45	360147.0
46	359798.0
47	359449.0
48	325925.5
49	292402.0
50	271095.0
51	249788.0
52	241684.0
53	233580.0
54	228300.5
55	223021.0
56	216726.5
57	210432.0
58	189745.5
59	169059.0
60	148148.0
61	127237.0
62	109849.5
63	92462.0
64	78895.5
65	65329.0
66	53876.0
67	42423.0
68	36128.0
69	29833.0
70	24703.0
71	19573.0
72	15669.5
73	11766.0
74	9102.5
75	5365.5
76	4292.0
77	3008.5
78	1725.0
79	1374.0
80	1023.0
81	619.0
82	215.0
83	210.5
84	206.0
85	144.0
86	82.0
87	69.5
88	57.0
89	62.0
90	67.0
91	37.0
92	7.0
93	6.5
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4201285.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.767276380827795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3722268024014	12.470196818534728
2	9.715261161782978	3.2579693802307466
3	3.6470251928726602	1.8345203813021307
4	1.8815170739916782	1.2619166717945955
5	1.2186801157094485	1.021697315995976
6	0.8319582044192573	0.8369803890476948
7	0.5944753324598923	0.6977412540657651
8	0.47361523036260117	0.6352989972527325
9	0.3836126725052504	0.5788925732775161
>10	3.6175525444356254	13.830731900874968
>50	1.6672862106646116	20.519574545330528
>100	1.5873260995359235	40.52015545976185
>500	0.005591984780111699	0.6319342265246595
>1k	0.00344122140314566	1.3101966727378491
>5k	2.8676845026213835E-4	0.3258602437251372
>10k+	1.4338422513106918E-4	0.2663331695430921
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11078	0.26368123086151024	No Hit
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	8308	0.19774902202540412	Illumina PCR Primer Index 5 (95% over 23bp)
ATGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	5246	0.12486655868383124	Illumina PCR Primer Index 5 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.140672437123404E-5	0.0	0.0	0.07559591886767977	0.0
2	7.140672437123404E-5	0.0	0.0	0.36127042083553007	0.0
3	7.140672437123404E-5	0.0	0.0	0.5047741345802534	0.0
4	9.520896582831205E-5	0.0	0.0	0.7347275892970841	0.0
5	9.520896582831205E-5	0.0	0.0	1.5845866205220545	0.0
6	9.520896582831205E-5	0.0	0.0	1.8347719804774016	0.0
7	1.1901120728539007E-4	0.0	0.0	2.219059168801926	0.0
8	1.1901120728539007E-4	0.0	0.0	3.3748484094747204	0.0
9	1.1901120728539007E-4	0.0	0.0	3.580428368939503	0.0
10	1.1901120728539007E-4	0.0	0.0	3.881883756993396	0.0
11	1.1901120728539007E-4	0.0	0.0	4.137353214552214	0.0
12	1.1901120728539007E-4	0.0	0.0	4.796008840152477	0.0
13	1.428134487424681E-4	0.0	0.0	5.344412483323555	0.0
14	1.666156901995461E-4	0.0	0.0	5.5716762847557355	0.0
15	1.904179316566241E-4	0.0	0.0	5.699875157243557	0.0
16	2.6182465602785814E-4	0.0	0.0	5.811721889850367	0.0
17	2.6182465602785814E-4	0.0	0.0	5.928019641609651	0.0
18	3.0942913894201415E-4	0.0	0.0	6.166565705492486	0.0
19	3.332313803990922E-4	0.0	0.0	6.466093111988356	0.0
20	4.5224258768448224E-4	0.0	0.0	6.787209151485795	0.0
21	4.7604482914156027E-4	0.0	0.0	7.012782993774524	0.0
22	4.7604482914156027E-4	0.0	0.0	7.272417843588331	0.0
23	4.7604482914156027E-4	0.0	0.0	7.658656815712336	0.0
24	4.7604482914156027E-4	0.0	0.0	8.02021286344535	0.0
25	4.7604482914156027E-4	0.0	0.0	8.388147911888863	0.0
26	4.998470705986383E-4	0.0	0.0	8.687556307177447	0.0
27	5.474515535127943E-4	0.0	0.0	8.95007122820756	0.0
28	5.950560364269503E-4	0.0	0.0	9.194401236764467	0.0
29	5.950560364269503E-4	0.0	0.0	9.449061418113743	0.0
30	5.950560364269503E-4	0.0	0.0	9.69884213996432	0.0
31	6.188582778840283E-4	0.0	0.0	9.978209047946045	0.0
32	6.664627607981844E-4	0.0	0.0	10.317533802158149	0.0
33	6.902650022552624E-4	0.0	0.0	10.603874766886797	0.0
34	7.140672437123404E-4	0.0	0.0	10.911090297373304	0.0
35	7.378694851694184E-4	0.0	0.0	11.206999763167698	0.0
36	7.616717266264964E-4	0.0	0.0	11.528639451977194	0.0
37	8.330784509977305E-4	0.0	0.0	11.893170779892342	0.0
38	8.806829339118864E-4	0.0	0.0	12.191650887764101	0.0
39	8.806829339118864E-4	0.0	0.0	12.509172788801521	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.03543E-4	45.0	14
ATTACGC	340	0.0	43.014706	1
ATACGTC	200	0.0	40.5	1
CGTTTTT	6385	0.0	39.714176	1
AGTCCGT	40	3.4611548E-7	39.375	41
GTACGAA	70	0.0	38.571426	19
TACGGGA	835	0.0	37.72455	6
ATCGGTT	400	0.0	37.6875	1
ATGCGAT	480	0.0	37.500004	1
ATGCGTT	1355	0.0	37.361626	1
GCTACGA	560	0.0	36.964283	10
AGGGCGA	4570	0.0	36.876366	8
TTACGGG	1375	0.0	36.81818	5
TTTACGG	730	0.0	36.67808	4
TACGTCC	80	0.0	36.5625	2
CGCGAAA	25	0.0021077443	36.0	30
TAATCCG	25	0.0021077443	36.0	3
CTACGAA	580	0.0	35.689655	11
TTGGGCG	7025	0.0	35.615658	7
GGGCGAT	20020	0.0	35.5482	9
>>END_MODULE