FastQCFastQC Report
Thu 26 May 2016
SRR1545581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545581_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3404151
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT82850.2433793330554373Illumina PCR Primer Index 5 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC76730.22540128214053962Illumina PCR Primer Index 5 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC67230.19749417696218527Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT64730.19015020191524995Illumina PCR Primer Index 5 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC62960.18495066758201972Illumina PCR Primer Index 5 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC53770.1579542153094854Illumina PCR Primer Index 5 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC50200.14746701894246173Illumina PCR Primer Index 5 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC48790.14332501701599018Illumina PCR Primer Index 5 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC44170.12975335112925368Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC253.418579E-546.036
TTTAGCG3900.040.692311
TCGTTGA6150.039.2682924
TTTACGG5100.039.235292
CGTAAAC301.00197925E-438.33333234
ATACCCG301.00197925E-438.33333242
ACGCGTT301.00197925E-438.33333225
AGGGCGA39200.038.0790826
TACGGGA7150.037.9580424
ATTATAC27350.037.92687242
ACATACG9450.037.7248717
CGAAATT552.220986E-937.63636437
CGGGTAT4300.037.4418646
TTGGGCG58400.037.178085
GGGCGAT168150.037.1364867
TGGGCGA93800.037.0255856
TTAATCG6600.036.93939220
GGCGATA168050.036.8574838
CACGTTA250.001892132536.80000316
GCGTAAT250.001892132536.80000331
Produced by FastQC (version 0.11.5)