##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545581_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3404151
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52051539429361	33.0	31.0	34.0	31.0	34.0
2	32.9296820264436	34.0	31.0	34.0	31.0	34.0
3	33.01690406800403	34.0	33.0	34.0	31.0	34.0
4	36.39623947351337	37.0	37.0	37.0	35.0	37.0
5	36.38021638875596	37.0	37.0	37.0	35.0	37.0
6	36.385029923760726	37.0	37.0	37.0	35.0	37.0
7	36.46362485095403	37.0	37.0	37.0	35.0	37.0
8	36.49847965028578	37.0	37.0	37.0	35.0	37.0
9	38.29446314220491	39.0	39.0	39.0	37.0	39.0
10	38.05120072523222	39.0	39.0	39.0	35.0	39.0
11	37.89033535821413	39.0	38.0	39.0	35.0	39.0
12	37.62936191725925	39.0	37.0	39.0	35.0	39.0
13	37.59258769660923	39.0	37.0	39.0	35.0	39.0
14	38.80648390744124	41.0	38.0	41.0	35.0	41.0
15	38.8953533494842	41.0	38.0	41.0	35.0	41.0
16	38.97469413078327	41.0	38.0	41.0	35.0	41.0
17	38.926876334216665	41.0	38.0	41.0	35.0	41.0
18	38.89928266989332	41.0	38.0	41.0	35.0	41.0
19	38.89451554881085	41.0	38.0	41.0	35.0	41.0
20	38.79674080262597	40.0	38.0	41.0	35.0	41.0
21	38.77120580138778	40.0	38.0	41.0	35.0	41.0
22	38.904532143256866	41.0	38.0	41.0	35.0	41.0
23	38.90792917235458	41.0	38.0	41.0	35.0	41.0
24	38.86810279567504	41.0	38.0	41.0	35.0	41.0
25	38.79591739614371	41.0	38.0	41.0	35.0	41.0
26	38.66983485750191	40.0	38.0	41.0	35.0	41.0
27	38.63039594894586	40.0	38.0	41.0	35.0	41.0
28	38.58329345554883	40.0	38.0	41.0	35.0	41.0
29	38.51667008895904	40.0	38.0	41.0	35.0	41.0
30	38.45177696289031	40.0	37.0	41.0	34.0	41.0
31	38.398609814899515	40.0	37.0	41.0	34.0	41.0
32	38.34739146412718	40.0	37.0	41.0	34.0	41.0
33	38.29992500332682	40.0	37.0	41.0	34.0	41.0
34	38.16731132079629	40.0	37.0	41.0	34.0	41.0
35	38.09504572505744	40.0	36.0	41.0	34.0	41.0
36	38.05917804468721	40.0	36.0	41.0	34.0	41.0
37	37.994075468450134	40.0	36.0	41.0	34.0	41.0
38	37.9318414488664	40.0	36.0	41.0	34.0	41.0
39	37.811325937069185	40.0	36.0	41.0	33.0	41.0
40	37.702243231866035	40.0	35.0	41.0	33.0	41.0
41	37.57216850838873	40.0	35.0	41.0	33.0	41.0
42	37.52648163962174	40.0	35.0	41.0	33.0	41.0
43	37.463950335928104	40.0	35.0	41.0	33.0	41.0
44	37.33729056084763	39.0	35.0	41.0	33.0	41.0
45	37.29402837888213	39.0	35.0	41.0	33.0	41.0
46	37.2604599502196	39.0	35.0	41.0	33.0	41.0
47	37.233306336881064	39.0	35.0	41.0	33.0	41.0
48	37.13822183563538	39.0	35.0	41.0	33.0	41.0
49	37.046624547500976	39.0	35.0	41.0	33.0	41.0
50	36.9442025339064	39.0	35.0	41.0	32.0	41.0
51	36.84096122645558	39.0	35.0	41.0	32.0	41.0
52	36.52206438551051	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	10.0
12	38.0
13	53.0
14	93.0
15	122.0
16	194.0
17	310.0
18	615.0
19	952.0
20	1611.0
21	2587.0
22	3688.0
23	5471.0
24	7242.0
25	9463.0
26	12348.0
27	15633.0
28	20314.0
29	25702.0
30	33448.0
31	43160.0
32	56599.0
33	79629.0
34	184962.0
35	262367.0
36	192754.0
37	286339.0
38	511308.0
39	1645012.0
40	2117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.46361662570197	13.994943232541681	44.73702841031435	11.804411731441995
2	26.54846979467127	13.117778852935725	48.00665422891053	12.327097123482478
3	27.396669536692116	14.190909862694104	43.71354267187325	14.698877928740528
4	24.258177736534016	14.468277112266758	45.98747235360594	15.286072797593292
5	24.010362642550227	16.77916167643562	43.44492944055654	15.765546240457606
6	25.66910222255123	19.50192573713681	43.15457804310091	11.674393997211052
7	86.85581221279548	2.919376960657738	6.434732184324374	3.790078642222393
8	87.29871265992607	3.1079408639628503	6.436465362435451	3.1568811136756274
9	79.76197295595877	5.483217401343243	8.672323877524821	6.082485765173167
10	40.57816471713505	26.098695386896765	15.074155053638924	18.248984842329264
11	31.290856369179863	23.01120014946458	27.072300846819076	18.625642634536483
12	27.300492839477446	23.99068078942444	28.15894477066382	20.549881600434293
13	24.77727926875159	23.150970682557855	33.1697389451878	18.90201110350275
14	19.281753365229687	28.99004773877539	32.284848703832466	19.443350192162452
15	19.66716517569285	24.407113550485864	35.55450389832883	20.37121737549245
16	24.93482222145845	23.96518250806148	30.664532801277026	20.435462469203042
17	25.138632216961	23.436269425181198	28.261907300821846	23.163191057035952
18	25.126764353285154	23.029501335281545	29.678413207874737	22.165321103558565
19	25.66081821869829	26.797371796962004	26.71670557504647	20.825104409293242
20	25.552362395205147	25.196120853628408	26.682130140525494	22.569386610640947
21	25.38174716691475	25.68749153606876	28.715588703321327	20.21517259369517
22	27.6904872903699	21.652887900683606	27.899702451506997	22.756922357439493
23	27.030645820352856	24.04296989175862	25.367382351722938	23.55900193616558
24	26.057069736330735	22.56744780122856	28.480581501819398	22.89490096062131
25	29.14054047543719	24.84925022421156	22.97530279943516	23.03490650091609
26	23.23478012579348	25.947115741928016	25.4779532400296	25.340150892248904
27	23.03293831560351	23.899821130143756	27.101823626507755	25.96541692774498
28	22.416602553764506	24.72319823650596	26.887849569540244	25.972349640189286
29	23.614845522422478	25.043630555753843	26.52288338560775	24.818640536215934
30	25.421639639369698	25.462648396031785	27.57674380484297	21.538968159755544
31	24.78353633549158	26.11214954918275	25.390853695972947	23.713460419352725
32	26.29016750432046	26.671907327260158	24.809534007157733	22.228391161261648
33	25.76295822365107	24.549527914596034	27.822796344815494	21.86471751693741
34	23.960805498933507	24.2622903625603	29.064104383148692	22.712799755357505
35	22.21872649009988	27.448282993321975	28.27500895230558	22.05798156427256
36	23.411623044923683	27.578653238355173	26.548763553673147	22.460960163047996
37	23.67788620422537	27.986126349859337	27.857753666038903	20.478233779876394
38	23.654473611775742	24.713416061743445	30.389368744218455	21.24274158226236
39	22.706718944018643	26.970366473167612	26.087297537623915	24.235617045189827
40	25.545811569463282	24.019469171608428	29.1292601297651	21.30545912916319
41	22.082686696330452	24.363167203805002	29.76245765831187	23.79168844155268
42	21.439236978618165	25.478100119530538	27.885073253213505	25.1975896486378
43	21.382394611754883	23.301463419219655	29.065220667355824	26.25092130166964
44	25.17347203458366	23.565229627005383	26.665356501518296	24.595941836892663
45	22.184650445882102	24.018852277704486	27.02656257022676	26.769934706186653
46	21.18475355529176	25.58749597182969	27.070009526604434	26.15774094627412
47	21.914069029252815	24.603462067340725	30.475528259469105	23.006940643937355
48	21.160606565337435	24.05416210973015	31.551244348444	23.23398697648841
49	22.24645733987711	23.823972555859008	27.894297285872454	26.03527281839143
50	20.887263814090502	24.297453315085026	30.91305291686532	23.90222995395915
51	22.323657205570495	24.112062009000187	29.13607533860866	24.428205446820662
52	21.38553783307497	23.693807942127126	29.291385722901246	25.629268501896657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	299.0
1	230.5
2	162.0
3	168.0
4	174.0
5	294.5
6	415.0
7	598.0
8	781.0
9	1161.5
10	1542.0
11	1633.0
12	1724.0
13	1853.5
14	2229.5
15	2476.0
16	2594.5
17	2713.0
18	3284.0
19	3855.0
20	5494.5
21	7134.0
22	9020.5
23	10907.0
24	12713.0
25	14519.0
26	21101.0
27	27683.0
28	30973.5
29	34264.0
30	45780.0
31	57296.0
32	66503.5
33	75711.0
34	85759.5
35	95808.0
36	103133.5
37	110459.0
38	120878.0
39	142344.0
40	153391.0
41	169534.5
42	185678.0
43	211014.5
44	236351.0
45	266360.0
46	296369.0
47	313695.0
48	331021.0
49	305153.5
50	279286.0
51	250774.0
52	222262.0
53	210896.5
54	199531.0
55	190095.5
56	180660.0
57	173915.0
58	167170.0
59	158811.0
60	150452.0
61	134057.5
62	117663.0
63	104142.5
64	80286.5
65	69951.0
66	59364.0
67	48777.0
68	40786.0
69	32795.0
70	27394.0
71	21993.0
72	18631.0
73	15269.0
74	13068.5
75	10868.0
76	8433.0
77	5998.0
78	4786.5
79	3575.0
80	2541.0
81	1507.0
82	1206.5
83	906.0
84	637.5
85	369.0
86	253.5
87	138.0
88	114.0
89	97.5
90	105.0
91	89.0
92	73.0
93	58.0
94	43.0
95	28.0
96	13.0
97	13.5
98	14.0
99	11.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3404151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.694420356980306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32953349737743	12.444403292129234
2	11.641076471779343	4.119642009988338
3	4.660803434164991	2.474106454961182
4	2.342178929745675	1.6577399413672886
5	1.41626096329682	1.2529958409877893
6	0.9064800645348073	0.9623783584260911
7	0.6776010814456208	0.8392830858610284
8	0.48620951936271856	0.6882556493735438
9	0.4131090365702341	0.6578752451706786
>10	3.928512930532567	16.67352380519319
>50	1.9181598601235046	24.883182627027246
>100	1.2708536237646193	30.465569947312055
>500	0.005532352381023907	0.6474502246381728
>1k	0.0025147056277381396	0.8735802100874898
>5k	0.0011735292929444653	1.3600133074766934
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	8285	0.2433793330554373	Illumina PCR Primer Index 5 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	7673	0.22540128214053962	Illumina PCR Primer Index 5 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	6723	0.19749417696218527	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT	6473	0.19015020191524995	Illumina PCR Primer Index 5 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	6296	0.18495066758201972	Illumina PCR Primer Index 5 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	5377	0.1579542153094854	Illumina PCR Primer Index 5 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	5020	0.14746701894246173	Illumina PCR Primer Index 5 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	4879	0.14332501701599018	Illumina PCR Primer Index 5 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	4417	0.12975335112925368	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20336935699973355	0.0
2	0.0	0.0	0.0	0.5731238126628343	0.0
3	0.0	0.0	0.0	2.1710552792752145	0.0
4	0.0	0.0	0.0	2.5872824090353217	0.0
5	0.0	0.0	0.0	3.275148487831474	0.0
6	0.0	0.0	0.0	5.439036047460879	0.0
7	2.9375900187741378E-5	0.0	0.0	5.833789394183748	0.0
8	2.9375900187741378E-5	0.0	0.0	6.400097998003027	0.0
9	5.8751800375482755E-5	0.0	0.0	6.836888257894553	0.0
10	5.8751800375482755E-5	0.0	0.0	8.082573305355726	0.0
11	5.8751800375482755E-5	0.0	0.0	9.089667291492063	0.0
12	5.8751800375482755E-5	0.0	0.0	9.512298367493099	0.0
13	8.812770056322414E-5	0.0	0.0	9.725097388453097	0.0
14	8.812770056322414E-5	0.0	0.0	9.897504546654952	0.0
15	8.812770056322414E-5	0.0	0.0	10.079664503719135	0.0
16	8.812770056322414E-5	0.0	0.0	10.474682233543694	0.0
17	8.812770056322414E-5	0.0	0.0	10.989729891535363	0.0
18	8.812770056322414E-5	0.0	0.0	11.531127731995438	0.0
19	8.812770056322414E-5	0.0	0.0	11.900441549155722	0.0
20	8.812770056322414E-5	0.0	0.0	12.333412942022843	0.0
21	8.812770056322414E-5	0.0	0.0	12.96008902072793	0.0
22	8.812770056322414E-5	0.0	0.0	13.550397735000592	0.0
23	8.812770056322414E-5	0.0	0.0	14.120290198642774	0.0
24	8.812770056322414E-5	0.0	0.0	14.587602018829365	0.0
25	8.812770056322414E-5	0.0	0.0	14.996338294041598	0.0
26	8.812770056322414E-5	0.0	0.0	15.365916494303573	0.0
27	8.812770056322414E-5	0.0	0.0	15.753472745480444	0.0
28	8.812770056322414E-5	0.0	0.0	16.12184653383472	0.0
29	8.812770056322414E-5	0.0	0.0	16.51988998137862	0.0
30	8.812770056322414E-5	0.0	0.0	16.99013939158398	0.0
31	8.812770056322414E-5	0.0	0.0	17.36814847519984	0.0
32	1.1750360075096551E-4	0.0	0.0	17.800679229564143	0.0
33	1.1750360075096551E-4	0.0	0.0	18.208152341068303	0.0
34	1.1750360075096551E-4	0.0	0.0	18.65105278819888	0.0
35	1.1750360075096551E-4	0.0	0.0	19.147916763974337	0.0
36	1.1750360075096551E-4	0.0	0.0	19.543698267203776	0.0
37	1.1750360075096551E-4	0.0	0.0	19.9709119836341	0.0
38	1.1750360075096551E-4	0.0	0.0	20.46680655470336	0.0
39	1.1750360075096551E-4	0.0	0.0	21.298203281816818	0.0
40	1.1750360075096551E-4	0.0	0.0	21.780261803897652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTAC	25	3.418579E-5	46.0	36
TTTAGCG	390	0.0	40.69231	1
TCGTTGA	615	0.0	39.26829	24
TTTACGG	510	0.0	39.23529	2
CGTAAAC	30	1.00197925E-4	38.333332	34
ATACCCG	30	1.00197925E-4	38.333332	42
ACGCGTT	30	1.00197925E-4	38.333332	25
AGGGCGA	3920	0.0	38.079082	6
TACGGGA	715	0.0	37.958042	4
ATTATAC	2735	0.0	37.926872	42
ACATACG	945	0.0	37.72487	17
CGAAATT	55	2.220986E-9	37.636364	37
CGGGTAT	430	0.0	37.441864	6
TTGGGCG	5840	0.0	37.17808	5
GGGCGAT	16815	0.0	37.136486	7
TGGGCGA	9380	0.0	37.025585	6
TTAATCG	660	0.0	36.939392	20
GGCGATA	16805	0.0	36.857483	8
CACGTTA	25	0.0018921325	36.800003	16
GCGTAAT	25	0.0018921325	36.800003	31
>>END_MODULE