FastQCFastQC Report
Thu 26 May 2016
SRR1545580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545580_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1276608
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC43480.34059006366872213No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30890.24196934376096657No Hit
ATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG25110.19669311174612725No Hit
ATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT19670.1540801874968667No Hit
ATGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC16030.12556712788890562No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT15860.1242354740061162No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC14900.11671554619742316No Hit
ATTGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC14610.11444390133854715No Hit
ATGGGGGGCATCATCTGTCCTTATAGCTCATTAGGAAGAGAAACAGTGTTG14340.11232892164235223No Hit
ATATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC14080.11029227452749787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.03358E-445.00000424
TTCGTAA900.045.0000042
CGATTAG207.03358E-445.00000444
CGTTCAC207.03358E-445.00000442
CCCACGT207.03358E-445.00000434
ATCGTAG800.045.0000041
CGTTAGC207.03358E-445.00000443
TACGCTA207.03358E-445.00000441
TCTACGA207.03358E-445.00000427
GAACGCT207.03358E-445.00000424
TTACGTA453.8562575E-1045.0000042
CCGAATC207.03358E-445.00000440
ACCCGGT207.03358E-445.00000422
ACCCGAT207.03358E-445.00000434
CACGTTA207.03358E-445.00000441
TATACGG750.045.0000044
CGATCAA207.03358E-445.00000444
CGATAGA453.8562575E-1045.00000413
ATCGCGA1250.045.0000041
AGTACGA453.8562575E-1045.00000428