##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545580_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1276608
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897801047776607	31.0	31.0	34.0	31.0	34.0
2	32.55400169825036	34.0	31.0	34.0	31.0	34.0
3	32.52224410312328	34.0	31.0	34.0	31.0	34.0
4	36.010683780769035	37.0	35.0	37.0	35.0	37.0
5	35.77274073168898	37.0	35.0	37.0	35.0	37.0
6	35.77640747982153	37.0	35.0	37.0	35.0	37.0
7	35.764959956384416	37.0	35.0	37.0	35.0	37.0
8	35.73731090514865	37.0	35.0	37.0	33.0	37.0
9	37.893569521732594	39.0	38.0	39.0	35.0	39.0
10	37.837369027924	39.0	38.0	39.0	35.0	39.0
11	37.68472076001404	39.0	38.0	39.0	35.0	39.0
12	37.20209727653281	39.0	37.0	39.0	33.0	39.0
13	36.86878744297388	39.0	37.0	39.0	33.0	39.0
14	37.50651100416103	39.0	35.0	41.0	33.0	41.0
15	37.342697993432594	40.0	35.0	41.0	32.0	41.0
16	37.39021297062215	40.0	35.0	41.0	32.0	41.0
17	37.46282570687321	40.0	35.0	41.0	32.0	41.0
18	37.526998107484836	39.0	35.0	41.0	33.0	41.0
19	37.47331757281797	39.0	35.0	41.0	32.0	41.0
20	37.428944515465986	39.0	35.0	41.0	32.0	41.0
21	37.40279239985963	39.0	35.0	41.0	32.0	41.0
22	37.33983885421367	39.0	35.0	41.0	32.0	41.0
23	37.26058038176167	39.0	35.0	41.0	32.0	41.0
24	37.211207355742715	39.0	35.0	41.0	32.0	41.0
25	37.15483923021006	39.0	35.0	41.0	32.0	41.0
26	37.05304682408382	39.0	35.0	41.0	32.0	41.0
27	36.972663495763776	39.0	35.0	41.0	32.0	41.0
28	36.874536271118465	39.0	35.0	41.0	31.0	41.0
29	36.806263159873666	39.0	35.0	41.0	31.0	41.0
30	36.75292415526144	39.0	35.0	41.0	31.0	41.0
31	36.68374003609566	38.0	35.0	41.0	31.0	41.0
32	36.563304475610366	38.0	35.0	41.0	31.0	41.0
33	36.46539893217025	38.0	35.0	41.0	31.0	41.0
34	36.346912286308715	38.0	35.0	41.0	30.0	41.0
35	36.25337926755903	38.0	35.0	41.0	30.0	41.0
36	36.149412348974785	38.0	35.0	41.0	30.0	41.0
37	36.03154609715747	38.0	35.0	40.0	30.0	41.0
38	35.95775993883792	38.0	35.0	40.0	30.0	41.0
39	35.89305331002156	38.0	35.0	40.0	30.0	41.0
40	35.80065141374643	38.0	35.0	40.0	30.0	41.0
41	35.71463988945706	37.0	35.0	40.0	29.0	41.0
42	35.58680581666416	37.0	34.0	40.0	29.0	41.0
43	35.47218645034341	37.0	34.0	40.0	29.0	41.0
44	35.379926336040505	37.0	34.0	40.0	29.0	41.0
45	35.316863908106484	37.0	34.0	40.0	28.0	41.0
46	35.236237748784276	36.0	34.0	40.0	28.0	41.0
47	35.160280211309974	36.0	34.0	40.0	28.0	41.0
48	35.10583280067178	36.0	34.0	40.0	28.0	41.0
49	35.06663987692385	36.0	34.0	40.0	28.0	41.0
50	34.96962497493357	36.0	34.0	40.0	28.0	41.0
51	34.78931747255226	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	6.0
11	15.0
12	26.0
13	26.0
14	33.0
15	40.0
16	79.0
17	187.0
18	396.0
19	716.0
20	1327.0
21	2102.0
22	3337.0
23	4705.0
24	6740.0
25	9487.0
26	12894.0
27	15670.0
28	18361.0
29	22199.0
30	26475.0
31	32622.0
32	40259.0
33	52673.0
34	88119.0
35	172984.0
36	96817.0
37	129090.0
38	189063.0
39	350012.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.0642985160675793	91.3285049130195	3.349422845540683	3.2577737253722363
2	3.3767609164285353	2.6909591667919988	91.07008572717702	2.8621941896024463
3	27.48110618138066	15.93543201985261	43.241778212262496	13.341683586504235
4	27.2681198927157	16.187036271118462	43.26731463377951	13.277529202386324
5	25.01245488043315	13.73632313129794	46.274110768536616	14.97711121973229
6	22.97126447586103	14.596258209254525	47.03158683009976	15.400890484784679
7	23.248013485737204	17.168543515315587	44.849476111696	14.733966887251215
8	26.49403732390836	19.288693161878978	42.03193149345766	12.185338020755001
9	82.48044818769739	4.408714343008974	9.034018273424575	4.076819195869053
10	83.6038940692836	4.517909961397704	8.464775404822781	3.4134205644959144
11	77.87300408582745	6.16923910863789	9.633262520679802	6.324494284854866
12	46.86450343410037	20.198291096405473	16.01047463277686	16.9267308367173
13	36.3023731638843	23.35344914022159	22.439072918233318	17.905104777660803
14	31.36890885847496	22.275592820975586	25.744472853060614	20.611025467488844
15	22.299405925703113	26.650859151752144	30.340558730636186	20.709176191908558
16	18.88880533413546	32.01248934676894	30.03498333082669	19.06372198826891
17	18.594509826039005	22.661459116659145	39.57369842582845	19.170332631473403
18	22.34319383867248	20.25437722464531	36.45042174261794	20.952007194064272
19	22.129502556775453	20.712622825487543	28.33743796059558	28.820436657141425
20	24.11954178573219	21.43437922995939	32.24513711335038	22.200941870958037
21	27.858042562791397	23.541134005113552	27.18618401263348	21.414639419461572
22	29.00381323005966	22.048036672181283	25.87763824133955	23.07051185641951
23	26.42627964104878	23.94376347320399	27.223861984258285	22.406094901488945
24	29.082694139469595	20.551571038251364	25.586241038752693	24.779493783526345
25	26.624069408933675	23.73649546297689	24.304250012533213	25.335185115556225
26	26.666604000601595	20.693274677896426	29.76935754750088	22.8707637740011
27	27.626569784930066	21.36309658093949	27.45940805634933	23.550925577781122
28	23.184015766781975	26.14396901789743	26.42048303002958	24.251532185291023
29	22.123940943500276	26.621876096656138	26.762169749837067	24.492013210006515
30	21.991480548453403	24.83260327367524	30.254079560836217	22.921836617035144
31	23.286396450594076	23.398882037399108	29.24343259638041	24.07128891562641
32	23.601450092745775	25.67099689176317	27.673491001153057	23.054062014337994
33	27.28942635484033	23.95480836717301	25.016293176918836	23.73947210106783
34	29.240142627964104	23.85023437108337	25.825782072492103	21.083840928460422
35	26.774467965107533	23.583355266456106	25.878343234571616	23.76383353386474
36	23.737670451696996	24.368091066325764	27.112159723266654	24.782078758710583
37	24.073012232415902	24.589615606356848	28.40096568406277	22.936406477164486
38	26.825070812653536	25.921974482378303	24.01998107484835	23.23297363011982
39	26.696370381511002	26.241179751341054	25.647497117361006	21.414952749786938
40	24.458565197774103	26.536180252669574	26.440379505690082	22.564875043866245
41	24.38618589261543	26.16175051386173	25.13590702862586	24.316156564896975
42	26.42103135809896	23.14249949867148	26.50711886499223	23.92935027823733
43	23.258275053892817	23.001970847746527	27.224488644909005	26.515265453451647
44	24.621026971474407	24.570972451997793	24.119855116057554	26.688145460470245
45	22.589393141825838	23.096283275680555	26.97076941394696	27.343554168546646
46	24.872161227252217	23.07035519125683	27.38930039604953	24.66818318544142
47	22.100441169098108	25.81230886850153	26.827734120419112	25.25951584198125
48	22.942359753346366	25.612795783827142	25.903331328019252	25.541513134807236
49	23.533379079560838	23.87138416804532	28.282761818819875	24.31247493357397
50	23.79038827893919	21.391922970872812	30.859277084273323	23.958411665914674
51	23.064950243144335	22.611561262345216	28.517524565097506	25.805963929412947
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	587.0
1	1229.5
2	1872.0
3	3777.5
4	5683.0
5	4139.5
6	2596.0
7	2453.5
8	2311.0
9	2430.0
10	2549.0
11	2863.5
12	3178.0
13	3189.0
14	3200.0
15	2979.5
16	2759.0
17	3022.0
18	3285.0
19	3205.0
20	3125.0
21	3744.5
22	4364.0
23	4839.0
24	5314.0
25	6267.0
26	8412.0
27	9604.0
28	11337.5
29	13071.0
30	15230.0
31	17389.0
32	18492.5
33	19596.0
34	25055.5
35	30515.0
36	32445.5
37	34376.0
38	46036.0
39	57696.0
40	68137.5
41	78579.0
42	86173.0
43	93767.0
44	100166.0
45	106565.0
46	114151.0
47	121737.0
48	112051.5
49	102366.0
50	91014.0
51	79662.0
52	77034.5
53	74407.0
54	73788.0
55	73169.0
56	73182.5
57	73196.0
58	68618.5
59	64041.0
60	58619.0
61	53197.0
62	47648.0
63	42099.0
64	36551.0
65	31003.0
66	25260.5
67	19518.0
68	16201.5
69	12885.0
70	10680.5
71	8476.0
72	7124.0
73	5772.0
74	4631.5
75	2439.0
76	1387.0
77	1023.0
78	659.0
79	437.0
80	215.0
81	127.0
82	39.0
83	30.0
84	21.0
85	17.5
86	14.0
87	29.5
88	45.0
89	25.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1276608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.22198171263331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07659036875343	11.854471125835135
2	10.957556170732184	3.5550655163353944
3	4.578446416334043	2.22814422114127
4	2.417334149829089	1.5685580148740583
5	1.3985005475730237	1.1343225153918628
6	0.8648506268864773	0.8417754632107132
7	0.5708344012871309	0.6482045653035275
8	0.39516432177746513	0.5128278721087345
9	0.28210493149489996	0.41186709357785445
>10	2.4522220851629934	9.068321339645724
>50	1.1422948310101606	13.752096838336335
>100	1.850052569812529	51.57352618017488
>500	0.006782072788000891	0.7451406722452161
>1k	0.0072665065585723835	2.1056785818192965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	4348	0.34059006366872213	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3089	0.24196934376096657	No Hit
ATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	2511	0.19669311174612725	No Hit
ATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1967	0.1540801874968667	No Hit
ATGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1603	0.12556712788890562	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1586	0.1242354740061162	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1490	0.11671554619742316	No Hit
ATTGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1461	0.11444390133854715	No Hit
ATGGGGGGCATCATCTGTCCTTATAGCTCATTAGGAAGAGAAACAGTGTTG	1434	0.11232892164235223	No Hit
ATATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	1408	0.11029227452749787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.833258134055246E-5	0.0	0.0	0.13308705569759863	0.0
2	7.833258134055246E-5	0.0	0.0	0.6378622098561187	0.0
3	7.833258134055246E-5	0.0	0.0	0.9294944101869955	0.0
4	7.833258134055246E-5	0.0	0.0	1.2516763172406877	0.0
5	7.833258134055246E-5	0.0	0.0	2.355930716398456	0.0
6	7.833258134055246E-5	0.0	0.0	2.6108249360806135	0.0
7	1.5666516268110492E-4	0.0	0.0	3.044552438963253	0.0
8	1.5666516268110492E-4	0.0	0.0	4.342209981450845	0.0
9	1.5666516268110492E-4	0.0	0.0	4.513445004261293	0.0
10	1.5666516268110492E-4	0.0	0.0	4.835705243896325	0.0
11	1.5666516268110492E-4	0.0	0.0	5.092479445530656	0.0
12	1.5666516268110492E-4	0.0	0.0	5.798725998897077	0.0
13	1.5666516268110492E-4	0.0	0.0	6.398988569709731	0.0
14	1.5666516268110492E-4	0.0	0.0	6.6458145335138115	0.0
15	1.5666516268110492E-4	0.0	0.0	6.78031157567554	0.0
16	1.5666516268110492E-4	0.0	0.0	6.866947410638192	0.0
17	1.5666516268110492E-4	0.0	0.0	6.994472853060611	0.0
18	1.5666516268110492E-4	0.0	0.0	7.271535193262145	0.0
19	1.5666516268110492E-4	0.0	0.0	7.611811926605505	0.0
20	1.5666516268110492E-4	0.0	0.0	8.020316338296485	0.0
21	1.5666516268110492E-4	0.0	0.0	8.272233919887702	0.0
22	1.5666516268110492E-4	0.0	0.0	8.55987115857021	0.0
23	2.3499774402165738E-4	0.0	0.0	8.980752118113	0.0
24	8.616583947460771E-4	0.0	0.0	9.342021983255627	0.0
25	9.399909760866295E-4	0.0	0.0	9.721621672431946	0.0
26	0.0010966561387677344	0.0	0.0	10.019520479270065	0.0
27	0.0010966561387677344	0.0	0.0	10.285616258083923	0.0
28	0.0010966561387677344	0.0	0.0	10.535732190304307	0.0
29	0.0010966561387677344	0.0	0.0	10.752478442873615	0.0
30	0.0010966561387677344	0.0	0.0	10.985987867849802	0.0
31	0.0011749887201082869	0.0	0.0	11.241195417857321	0.0
32	0.0011749887201082869	0.0	0.0	11.52710933975034	0.0
33	0.0011749887201082869	0.0	0.0	11.800333383466185	0.0
34	0.0012533213014488394	0.0	0.0	12.0717557778112	0.0
35	0.0012533213014488394	0.0	0.0	12.352734747079761	0.0
36	0.0012533213014488394	0.0	0.0	12.619065523637639	0.0
37	0.0012533213014488394	0.0	0.0	12.92683423572467	0.0
38	0.0012533213014488394	0.0	0.0	13.222226399959894	0.0
39	0.0012533213014488394	0.0	0.0	13.509785306061062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	20	7.03358E-4	45.000004	24
TTCGTAA	90	0.0	45.000004	2
CGATTAG	20	7.03358E-4	45.000004	44
CGTTCAC	20	7.03358E-4	45.000004	42
CCCACGT	20	7.03358E-4	45.000004	34
ATCGTAG	80	0.0	45.000004	1
CGTTAGC	20	7.03358E-4	45.000004	43
TACGCTA	20	7.03358E-4	45.000004	41
TCTACGA	20	7.03358E-4	45.000004	27
GAACGCT	20	7.03358E-4	45.000004	24
TTACGTA	45	3.8562575E-10	45.000004	2
CCGAATC	20	7.03358E-4	45.000004	40
ACCCGGT	20	7.03358E-4	45.000004	22
ACCCGAT	20	7.03358E-4	45.000004	34
CACGTTA	20	7.03358E-4	45.000004	41
TATACGG	75	0.0	45.000004	4
CGATCAA	20	7.03358E-4	45.000004	44
CGATAGA	45	3.8562575E-10	45.000004	13
ATCGCGA	125	0.0	45.000004	1
AGTACGA	45	3.8562575E-10	45.000004	28
>>END_MODULE