FastQCFastQC Report
Thu 26 May 2016
SRR1545579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545579_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1053900
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT46050.4369484770851124Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT30820.2924376126767245No Hit
TGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC28560.2709934528892684No Hit
GTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC26560.2520163203339975No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC24980.2370243856153335No Hit
TTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC22500.2134927412467976No Hit
ATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC21460.20362463231805678No Hit
GGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC20430.19385140905209222No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC15600.148021633931113No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT11690.11092133978555839No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC11230.10655659929784611No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG206.3112134E-446.00000445
TAATACG206.3112134E-446.0000041
ACTATCG405.6097633E-946.00000443
AATCCGT206.3112134E-446.00000426
CCTTACG206.3112134E-446.0000041
TGCCCGT206.3112134E-446.00000444
ACCGGAT206.3112134E-446.00000426
CTACTGA206.3112134E-446.00000441
GGTCGAC405.6097633E-946.0000049
CCGATTC206.3112134E-446.00000445
CGGCATA206.3112134E-446.00000444
TTAGACT206.3112134E-446.00000445
CGTGACA206.3112134E-446.00000438
CCGTGTA351.0194708E-746.00000419
GCACGTC351.0194708E-746.0000049
ACCGAAT206.3112134E-446.00000411
TATATCG206.3112134E-446.00000413
ATTAACG206.3112134E-446.0000041
ATTCGGT206.3112134E-446.00000419
CCCACGT206.3112134E-446.00000432
Produced by FastQC (version 0.11.5)