SRR1545578_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545578_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1130040
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	7946	0.7031609500548653	TruSeq Adapter, Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3705	0.32786450037166825	No Hit
ATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	3396	0.3005203355633429	TruSeq Adapter, Index 8 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	3172	0.28069802838837565	TruSeq Adapter, Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	2456	0.21733743938267672	TruSeq Adapter, Index 8 (95% over 24bp)
ATGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2438	0.2157445754132597	No Hit
ATATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2341	0.2071608084669569	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2228	0.1971611624367279	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2089	0.18486071289511877	TruSeq Adapter, Index 13 (95% over 23bp)
ATGCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1997	0.17671940816254292	No Hit
ATGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1753	0.15512725213266787	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1739	0.15388835793423242	No Hit
ATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1550	0.1371632862553538	No Hit
ATTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1424	0.1260132384694347	No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG	1401	0.12397791228629075	No Hit
ATGTGTGTGTGTGTTTAATGGATATTTTTCAGACAATATATTTTGATTATG	1223	0.10822625747761142	No Hit
ATCGCGATGGGAGTGCGTCTTTAGTAAGGATGTGTTAAAATATAGCAGTCT	1210	0.10707585572192135	No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1142	0.10105836961523487	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGT	35	1.2119017E-7	45.000004	16
GATACTA	35	1.2119017E-7	45.000004	27
AACCGGC	90	0.0	45.000004	19
TTGGCCG	45	3.8562575E-10	45.000004	2
CAAACCG	35	1.2119017E-7	45.000004	33
CGTATTA	35	1.2119017E-7	45.000004	22
ATTAGTC	140	0.0	45.000004	1
AGTTTCG	35	1.2119017E-7	45.000004	12
TACTTGA	45	3.8562575E-10	45.000004	14
TACTTCG	70	0.0	45.000004	30
CGACGTG	35	1.2119017E-7	45.000004	18
ACCATTC	45	3.8562575E-10	45.000004	41
ACGTTCA	35	1.2119017E-7	45.000004	45
CTATCGG	70	0.0	45.000004	31
CTATCCG	45	3.8562575E-10	45.000004	42
GCTATCG	70	0.0	45.000004	30
GCTATCC	45	3.8562575E-10	45.000004	41
GCAGTGC	70	0.0	45.000004	41
CGACCGT	35	1.2119017E-7	45.000004	5
ATTCGAC	140	0.0	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content