##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545578_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1130040
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843974549573467	31.0	31.0	34.0	31.0	34.0
2	32.531737814590635	34.0	31.0	34.0	31.0	34.0
3	32.470072740787934	34.0	31.0	34.0	31.0	34.0
4	35.939032246646136	37.0	35.0	37.0	35.0	37.0
5	35.700503521999224	37.0	35.0	37.0	35.0	37.0
6	35.68568723231036	37.0	35.0	37.0	33.0	37.0
7	35.67342837421684	37.0	35.0	37.0	33.0	37.0
8	35.632040458744825	37.0	35.0	37.0	33.0	37.0
9	37.856901525609715	39.0	38.0	39.0	35.0	39.0
10	37.81232788219886	39.0	38.0	39.0	35.0	39.0
11	37.60858111217302	39.0	37.0	39.0	35.0	39.0
12	37.13993221478886	39.0	37.0	39.0	33.0	39.0
13	36.8521919578068	39.0	35.0	39.0	33.0	39.0
14	37.14699037202223	39.0	35.0	41.0	32.0	41.0
15	36.81846129340554	39.0	35.0	41.0	31.0	41.0
16	36.883367845386005	39.0	35.0	41.0	31.0	41.0
17	37.09764521609854	39.0	35.0	41.0	32.0	41.0
18	37.21015185303175	39.0	35.0	41.0	32.0	41.0
19	37.18762344695763	39.0	35.0	41.0	32.0	41.0
20	37.155752893702875	39.0	35.0	41.0	32.0	41.0
21	37.13703143251566	39.0	35.0	41.0	32.0	41.0
22	37.02250893773672	38.0	35.0	41.0	32.0	41.0
23	36.92646454992744	38.0	35.0	41.0	32.0	41.0
24	36.84386127924675	38.0	35.0	41.0	32.0	41.0
25	36.82940692364872	38.0	35.0	41.0	32.0	41.0
26	36.72748663764114	38.0	35.0	41.0	31.0	41.0
27	36.619718771016956	38.0	35.0	41.0	31.0	41.0
28	36.55326094651517	38.0	35.0	41.0	31.0	41.0
29	36.494196665604754	38.0	35.0	41.0	31.0	41.0
30	36.35774397366465	38.0	35.0	41.0	31.0	41.0
31	36.36993203780397	38.0	35.0	41.0	31.0	41.0
32	36.25316625960143	38.0	35.0	40.0	30.0	41.0
33	36.10108403242363	38.0	35.0	40.0	30.0	41.0
34	36.03555714841953	38.0	35.0	40.0	30.0	41.0
35	35.906250221231105	38.0	35.0	40.0	30.0	41.0
36	35.83713054405154	38.0	35.0	40.0	30.0	41.0
37	35.7532556369686	37.0	35.0	40.0	30.0	41.0
38	35.64742929453825	37.0	35.0	40.0	29.0	41.0
39	35.56777459204984	37.0	34.0	40.0	29.0	41.0
40	35.47885207603271	37.0	34.0	40.0	29.0	41.0
41	35.42451152171605	37.0	34.0	40.0	29.0	41.0
42	35.3089899472585	37.0	34.0	40.0	28.0	41.0
43	35.26117748044317	37.0	34.0	40.0	29.0	41.0
44	35.19801511450922	36.0	34.0	40.0	28.0	41.0
45	35.1913808360766	36.0	34.0	40.0	28.0	41.0
46	35.119343563059715	36.0	34.0	40.0	28.0	41.0
47	34.99287901313228	36.0	34.0	40.0	28.0	41.0
48	34.96777547697427	36.0	34.0	40.0	27.0	41.0
49	34.93894021450568	36.0	34.0	40.0	28.0	41.0
50	34.81715602987505	35.0	34.0	40.0	27.0	41.0
51	34.71602332660791	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	6.0
12	10.0
13	10.0
14	16.0
15	37.0
16	67.0
17	182.0
18	334.0
19	664.0
20	1199.0
21	1918.0
22	2929.0
23	4679.0
24	6526.0
25	9378.0
26	12605.0
27	15242.0
28	17737.0
29	20500.0
30	24934.0
31	30974.0
32	38422.0
33	49848.0
34	83743.0
35	185904.0
36	78677.0
37	103816.0
38	150501.0
39	289054.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.9744433825351313	90.99014194187816	3.2793529432586457	3.7560617323280594
2	3.7041166684365154	2.5881384729744084	90.5460868641818	3.161657994407278
3	27.991664011893384	16.32499734522672	42.88927825563697	12.794060387242927
4	25.770946161197834	15.450780503345014	44.81195355916604	13.966319776291106
5	24.397100987575662	13.392534777529999	47.008247495663866	15.20211673923047
6	22.347085058935967	14.600014158790838	48.11157127181339	14.941329510459806
7	22.327528229089236	16.590740150791124	45.370694842660434	15.711036777459206
8	24.1544547095678	18.34280202470709	45.239991504725495	12.26275176099961
9	79.9531874977877	3.874199143393154	11.0258928887473	5.146720470071856
10	82.21293051573396	4.063661463311033	10.067254256486496	3.6561537644685145
11	75.02362748221302	7.382570528476869	10.969434710275742	6.624367279034371
12	47.71547909808503	18.88711904003398	16.78940568475452	16.607996177126473
13	39.27772468231213	21.700205302467168	23.03980390074688	15.982266114473823
14	33.23784998761106	23.532529821953204	24.7869101978691	18.442709992566638
15	19.71195709886376	34.323652260096985	30.7112137623447	15.25317687869456
16	15.192648047856713	38.306431630738736	29.931418356872324	16.56950196453223
17	13.846943471027572	24.68638278291034	42.98794732929807	18.478726416764008
18	17.028246787724328	20.403614031361723	42.163817210010265	20.404321970903684
19	17.253106084740363	19.65178223779689	30.052210541219782	33.04290113624296
20	20.74838058829776	21.371278892782556	35.56405083005912	22.31628968886057
21	25.258486425259285	22.402923790308307	29.498247849633643	22.84034193479877
22	26.292078156525434	21.842501150401755	27.759371349686734	24.106049343386076
23	23.552352129128174	26.181462603093696	29.44258610314679	20.82359916463134
24	26.78445010796078	21.699939825138934	25.082828926409682	26.4327811404906
25	24.23073519521433	25.161852677781315	23.636065979965313	26.971346147039043
26	22.399472585041238	21.037396906304203	33.734558068740924	22.82857243991363
27	23.67332129836112	23.787653534388163	30.698559343032105	21.84046582421861
28	20.092474602668933	29.36126154826378	26.319068351562773	24.227195497504514
29	20.740062298679693	28.88313688011044	25.43166613571201	24.94513468549786
30	19.33834200559272	28.659958939506563	31.873031043148913	20.128668011751795
31	19.88159711160667	23.46890375561927	30.862358854553822	25.78714027822024
32	21.860022654065343	27.521769140915364	27.495132915649002	23.123075289370288
33	24.807174967257797	29.14171179781247	22.766185267778134	23.284927967151607
34	28.035379278609607	24.979912215496796	24.717709107642207	22.26699939825139
35	26.344996637287178	26.050316802945026	24.880358217408233	22.724328342359563
36	21.277742380800678	27.19346217832997	24.70939081802414	26.819404622845212
37	21.25641570209904	23.345014335775723	28.832961665073803	26.56560829705143
38	28.21917808219178	24.61019078970656	25.483434214718066	21.6871969133836
39	24.81593571908959	26.099341616225974	25.996955859969557	23.087766804714878
40	22.22682382924498	28.018211744717004	26.215178223779688	23.539786202258327
41	20.54449400021238	27.931135181055538	26.524901773388553	24.999469045343528
42	24.484354536122616	22.796626668082546	26.47693886942055	26.24207992637429
43	20.657675834483737	22.44203745000177	28.349350465470245	28.550936250044245
44	21.369243566599412	24.214541078191925	26.126951258362535	28.28926409684613
45	21.196063856146687	22.839545502814058	28.00838908357226	27.95600155746699
46	23.56412162401331	25.899171710735903	26.364022512477437	24.172684152773353
47	19.231620119641782	28.895525822094793	26.262167710877492	25.610686347385936
48	22.351332696187747	25.96120491310042	25.77917595837316	25.908286432338677
49	22.693621464726913	24.110827935294328	29.430285653605182	23.76526494637358
50	22.920073625712366	21.9923188559697	31.664454355598032	23.423153162719903
51	21.447205408658103	20.877579554706028	29.414445506353758	28.260769530282115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	897.0
1	1107.0
2	1317.0
3	3514.0
4	5711.0
5	3995.5
6	2280.0
7	2404.0
8	2528.0
9	2658.0
10	2788.0
11	2835.0
12	2882.0
13	2898.0
14	2914.0
15	2988.5
16	3063.0
17	3417.5
18	3772.0
19	3091.5
20	2411.0
21	2399.5
22	2388.0
23	3640.5
24	4893.0
25	6616.5
26	8510.0
27	8680.0
28	10569.0
29	12458.0
30	12562.5
31	12667.0
32	15067.0
33	17467.0
34	21843.5
35	26220.0
36	30005.0
37	33790.0
38	43436.0
39	53082.0
40	67982.0
41	82882.0
42	88214.5
43	93547.0
44	97389.5
45	101232.0
46	108220.0
47	115208.0
48	122290.0
49	129372.0
50	118507.5
51	107643.0
52	89495.5
53	71348.0
54	61675.5
55	52003.0
56	50479.0
57	48955.0
58	43082.0
59	37209.0
60	33539.0
61	29869.0
62	23728.5
63	17588.0
64	15041.0
65	12494.0
66	10081.5
67	7669.0
68	7122.0
69	6575.0
70	5177.5
71	3780.0
72	2357.0
73	934.0
74	812.5
75	478.0
76	265.0
77	220.5
78	176.0
79	97.0
80	18.0
81	21.0
82	24.0
83	15.0
84	6.0
85	3.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1130040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.525513531243607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17095390781817	10.894400943961026
2	11.452742195979202	3.5561940786703947
3	5.372718053148367	2.5024262050113535
4	3.013381541471569	1.8713718358766622
5	1.9246975969440798	1.4940959292453684
6	1.3252674336861443	1.2345274484526434
7	0.8598140038484127	0.934433776577089
8	0.6490615027727442	0.8061610515126041
9	0.4784905139980311	0.6685929854683327
>10	2.8102257575980434	8.405492379433433
>50	0.4564878383989104	5.102345779916864
>100	1.4220911857890837	53.53106833851431
>500	0.05205563069461247	4.877118213854139
>1k	0.011440797954859885	3.41606983479351
>5k	5.720398977429942E-4	0.7057011987122824
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	7946	0.7031609500548653	TruSeq Adapter, Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3705	0.32786450037166825	No Hit
ATGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	3396	0.3005203355633429	TruSeq Adapter, Index 8 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG	3172	0.28069802838837565	TruSeq Adapter, Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	2456	0.21733743938267672	TruSeq Adapter, Index 8 (95% over 24bp)
ATGTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2438	0.2157445754132597	No Hit
ATATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2341	0.2071608084669569	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	2228	0.1971611624367279	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2089	0.18486071289511877	TruSeq Adapter, Index 13 (95% over 23bp)
ATGCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1997	0.17671940816254292	No Hit
ATGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1753	0.15512725213266787	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1739	0.15388835793423242	No Hit
ATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1550	0.1371632862553538	No Hit
ATTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1424	0.1260132384694347	No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG	1401	0.12397791228629075	No Hit
ATGTGTGTGTGTGTTTAATGGATATTTTTCAGACAATATATTTTGATTATG	1223	0.10822625747761142	No Hit
ATCGCGATGGGAGTGCGTCTTTAGTAAGGATGTGTTAAAATATAGCAGTCT	1210	0.10707585572192135	No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1142	0.10105836961523487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24061095182471418	0.0
2	0.0	0.0	0.0	1.2403100775193798	0.0
3	0.0	0.0	0.0	1.6470213443771902	0.0
4	0.0	0.0	0.0	2.2332837775653958	0.0
5	0.0	0.0	0.0	4.2923259353651195	0.0
6	0.0	0.0	0.0	4.758946585961559	0.0
7	0.0	0.0	0.0	5.548210682807688	0.0
8	0.0	0.0	0.0	7.806449329227284	0.0
9	0.0	0.0	0.0	8.103606951966302	0.0
10	0.0	0.0	0.0	8.705886517291423	0.0
11	0.0	0.0	0.0	9.137641145446178	0.0
12	0.0	0.0	0.0	10.29981239602138	0.0
13	8.849244274538954E-5	0.0	0.0	11.250132738664117	0.0
14	8.849244274538954E-5	0.0	0.0	11.681533397047891	0.0
15	8.849244274538954E-5	0.0	0.0	11.930816608261654	0.0
16	1.7698488549077907E-4	0.0	0.0	12.058953665356979	0.0
17	1.7698488549077907E-4	0.0	0.0	12.249123924816821	0.0
18	1.7698488549077907E-4	0.0	0.0	12.662118155109553	0.0
19	1.7698488549077907E-4	0.0	0.0	13.207143109978407	0.0
20	2.654773282361686E-4	0.0	0.0	13.89366748079714	0.0
21	2.654773282361686E-4	0.0	0.0	14.264450815900322	0.0
22	3.5396977098155815E-4	0.0	0.0	14.683993486956213	0.0
23	4.424622137269477E-4	0.0	0.0	15.317422392127712	0.0
24	4.424622137269477E-4	0.0	0.0	15.840412728752964	0.0
25	5.309546564723373E-4	0.0	0.0	16.440037520795723	0.0
26	7.079395419631163E-4	0.0	0.0	16.84418250681392	0.0
27	7.079395419631163E-4	0.0	0.0	17.211957098863756	0.0
28	7.964319847085059E-4	0.0	0.0	17.566988779158258	0.0
29	8.849244274538955E-4	0.0	0.0	17.878039715408303	0.0
30	8.849244274538955E-4	0.0	0.0	18.189179144101093	0.0
31	8.849244274538955E-4	0.0	0.0	18.521379774167286	0.0
32	8.849244274538955E-4	0.0	0.0	18.91340129552936	0.0
33	8.849244274538955E-4	0.0	0.0	19.27790166719762	0.0
34	9.73416870199285E-4	0.0	0.0	19.616385260698735	0.0
35	9.73416870199285E-4	0.0	0.0	19.971682418321475	0.0
36	9.73416870199285E-4	0.0	0.0	20.321758521822236	0.0
37	9.73416870199285E-4	0.0	0.0	20.737849987611057	0.0
38	9.73416870199285E-4	0.0	0.0	21.122526636225267	0.0
39	0.0010619093129446745	0.0	0.0	21.478531733389968	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGT	35	1.2119017E-7	45.000004	16
GATACTA	35	1.2119017E-7	45.000004	27
AACCGGC	90	0.0	45.000004	19
TTGGCCG	45	3.8562575E-10	45.000004	2
CAAACCG	35	1.2119017E-7	45.000004	33
CGTATTA	35	1.2119017E-7	45.000004	22
ATTAGTC	140	0.0	45.000004	1
AGTTTCG	35	1.2119017E-7	45.000004	12
TACTTGA	45	3.8562575E-10	45.000004	14
TACTTCG	70	0.0	45.000004	30
CGACGTG	35	1.2119017E-7	45.000004	18
ACCATTC	45	3.8562575E-10	45.000004	41
ACGTTCA	35	1.2119017E-7	45.000004	45
CTATCGG	70	0.0	45.000004	31
CTATCCG	45	3.8562575E-10	45.000004	42
GCTATCG	70	0.0	45.000004	30
GCTATCC	45	3.8562575E-10	45.000004	41
GCAGTGC	70	0.0	45.000004	41
CGACCGT	35	1.2119017E-7	45.000004	5
ATTCGAC	140	0.0	45.000004	1
>>END_MODULE