FastQCFastQC Report
Thu 26 May 2016
SRR1545577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545577_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences968652
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT58110.5999058485400329TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT52560.5426097298100866TruSeq Adapter, Index 13 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC51350.5301181435644586TruSeq Adapter, Index 13 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC42230.43596668359741164TruSeq Adapter, Index 8 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC39750.4103640936063726TruSeq Adapter, Index 8 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC37740.38961360736363526TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC31870.32901392863484513TruSeq Adapter, Index 13 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC29650.3060954811428666TruSeq Adapter, Index 13 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC24760.2556129549105355TruSeq Adapter, Index 8 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC20520.21184078492585573TruSeq Adapter, Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT16470.17003010369048946TruSeq Adapter, Index 13 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC15900.16414563744254904TruSeq Adapter, Index 8 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC14520.14989903494753534TruSeq Adapter, Index 8 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC11640.12016699495794156No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT11580.11954757745815835TruSeq Adapter, Index 13 (95% over 24bp)
GACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC11320.11686343495909779TruSeq Adapter, Index 8 (95% over 23bp)
AACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC10990.11345663871029019TruSeq Adapter, Index 13 (95% over 23bp)
GTGTGTGTGTGTTTAATGGATATTTTTCAGACAATATATTTTGATTATGTTC10780.11128867746104897No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCA9800.10117152496458998No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT9730.1004488712148429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT351.0193435E-746.0000041
AACGTGC351.0193435E-746.00000440
TGTCAGT351.0193435E-746.00000420
CAGGTAG351.0193435E-746.00000411
AGGTAGT351.0193435E-746.00000414
TCGTTGA850.046.00000424
AATCCCC351.0193435E-746.00000445
AAATCTC351.0193435E-746.00000444
TGCCCGC351.0193435E-746.00000427
ACCGGAC351.0193435E-746.00000425
GTCGATA351.0193435E-746.0000048
CGTGCCG351.0193435E-746.00000430
CAACCGT351.0193435E-746.00000437
TTGTACG351.0193435E-746.0000041
TACGGTA351.0193435E-746.00000437
GGTCAAC351.0193435E-746.0000048
CGACATA351.0193435E-746.00000429
TCGATTC351.0193435E-746.00000438
TAACGAT351.0193435E-746.00000415
TCGATCC351.0193435E-746.00000412
Produced by FastQC (version 0.11.5)