##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545577_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	968652
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52498110776626	33.0	31.0	34.0	31.0	34.0
2	32.90843976990705	34.0	31.0	34.0	31.0	34.0
3	32.97094312508517	34.0	31.0	34.0	31.0	34.0
4	36.35680099767512	37.0	37.0	37.0	35.0	37.0
5	36.332092433608764	37.0	37.0	37.0	35.0	37.0
6	36.33159380252144	37.0	37.0	37.0	35.0	37.0
7	36.44173862233289	37.0	37.0	37.0	35.0	37.0
8	36.49617922638884	37.0	37.0	37.0	35.0	37.0
9	38.27443808509145	39.0	39.0	39.0	37.0	39.0
10	38.04025284622341	39.0	39.0	39.0	35.0	39.0
11	37.85735021452493	39.0	38.0	39.0	35.0	39.0
12	37.23933776010373	39.0	35.0	39.0	35.0	39.0
13	37.055707312843005	39.0	35.0	39.0	34.0	39.0
14	38.27584313045345	40.0	35.0	41.0	34.0	41.0
15	38.365361347522125	40.0	35.0	41.0	35.0	41.0
16	38.450425952767354	40.0	35.0	41.0	35.0	41.0
17	38.41505824589223	40.0	35.0	41.0	35.0	41.0
18	38.43608850237237	40.0	36.0	41.0	35.0	41.0
19	38.42744659588789	40.0	36.0	41.0	35.0	41.0
20	38.31338396039032	40.0	36.0	41.0	35.0	41.0
21	38.25506270569823	40.0	35.0	41.0	35.0	41.0
22	38.33247544009613	40.0	35.0	41.0	35.0	41.0
23	38.335717058345	40.0	35.0	41.0	35.0	41.0
24	38.27922411763977	40.0	35.0	41.0	35.0	41.0
25	38.19905084591783	40.0	35.0	41.0	35.0	41.0
26	38.11951247713317	40.0	35.0	41.0	35.0	41.0
27	38.07188340084984	40.0	35.0	41.0	34.0	41.0
28	37.976766681945634	40.0	35.0	41.0	34.0	41.0
29	37.99298406445246	40.0	35.0	41.0	34.0	41.0
30	37.91467110995487	40.0	35.0	41.0	34.0	41.0
31	37.80446641311843	40.0	35.0	41.0	34.0	41.0
32	37.76858768680599	40.0	35.0	41.0	34.0	41.0
33	37.675331285126134	40.0	35.0	41.0	34.0	41.0
34	37.60185494893935	40.0	35.0	41.0	34.0	41.0
35	37.62670907611815	40.0	35.0	41.0	34.0	41.0
36	37.54622609564632	40.0	35.0	41.0	34.0	41.0
37	37.43594087453492	40.0	35.0	41.0	33.0	41.0
38	37.33110859214661	39.0	35.0	41.0	33.0	41.0
39	37.27262835362958	39.0	35.0	41.0	33.0	41.0
40	37.17390662487663	39.0	35.0	41.0	33.0	41.0
41	37.11121125027358	39.0	35.0	41.0	33.0	41.0
42	37.10263335026408	39.0	35.0	41.0	33.0	41.0
43	37.0242832307165	39.0	35.0	41.0	33.0	41.0
44	36.90024074693491	38.0	35.0	41.0	33.0	41.0
45	36.77419444754153	38.0	35.0	41.0	33.0	41.0
46	36.7939858690221	38.0	35.0	41.0	33.0	41.0
47	36.802233413031715	38.0	35.0	41.0	33.0	41.0
48	36.700409434967355	38.0	35.0	40.0	33.0	41.0
49	36.696179845806334	38.0	35.0	40.0	33.0	41.0
50	36.57525096732366	38.0	35.0	40.0	33.0	41.0
51	36.43389473206064	37.0	35.0	40.0	32.0	41.0
52	36.100106126864965	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	8.0
14	17.0
15	32.0
16	33.0
17	52.0
18	109.0
19	230.0
20	407.0
21	630.0
22	984.0
23	1603.0
24	2416.0
25	3299.0
26	4138.0
27	4922.0
28	6160.0
29	8065.0
30	10091.0
31	13153.0
32	17286.0
33	24897.0
34	59950.0
35	155264.0
36	54536.0
37	70780.0
38	123700.0
39	405319.0
40	568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.95317616646639	14.486626776179682	45.61669206278416	11.943504994569773
2	24.081713556571398	13.44610861279386	50.11882492370842	12.353352906926327
3	25.485107138580215	13.355054240325732	44.68983701060856	16.4700016104855
4	22.84711124325351	14.994239417252016	47.821818362012365	14.33683097748211
5	23.133178891903388	16.27674335055314	44.210511102026324	16.379566655517152
6	22.941778884470377	18.002337268699183	46.16745745634139	12.88842639048905
7	82.25719866370999	2.705822111552962	8.787676069424313	6.249303155312743
8	84.91460297402989	2.5608784166036926	8.553845963256155	3.970672646110265
9	76.23935118081623	4.882351969541176	10.790046373723484	8.088250475919113
10	46.71977139364808	18.1725738448896	15.55491549080578	19.55273927065654
11	38.16706102914153	19.48811337817916	25.8237220384617	16.521103554217614
12	32.663846252317654	23.214735529374842	24.79125630257306	19.330161915734443
13	20.04331793048484	31.42563067025103	33.50501521702324	15.026036182240887
14	14.98577404475498	38.91283969888051	29.441636418445427	16.659749837919087
15	13.317476245338883	24.53182360641386	41.690720712908245	20.459979435339008
16	16.580154689196945	21.607243881187465	40.648034588273184	21.164566841342403
17	16.54226698546021	21.40634613875778	28.003761928948684	34.04762494683333
18	19.528169043165143	22.449238735892767	33.15411520339606	24.868477017546034
19	23.00010736569996	24.223043982771937	29.567068462151525	23.209780189376577
20	25.618075428533672	20.6568509640201	26.872602338094588	26.852471269351636
21	22.740881142040692	27.097244418015965	29.750416042087352	20.411458397855988
22	26.281058625801627	20.948390133918064	25.0848601974703	27.685691042810006
23	24.325351106486124	25.820005533462997	21.63862770117648	28.216015658874394
24	22.44717401089349	19.923563880526753	32.62348087858179	25.005781229997975
25	26.125894542105936	22.389464947163688	28.031429243938998	23.453211266791378
26	18.12209131865727	29.507294673422447	25.72688643599559	26.643727571924696
27	20.06148751047848	27.01011302304646	23.838385715406567	29.090013751068494
28	20.98235486015618	27.28059199795179	30.25410570566106	21.48294743623097
29	21.714609581149887	22.29262934469758	28.184528602635417	27.80823247151712
30	23.149696691897606	27.52443602036645	24.65704917762003	24.668818110115915
31	23.998505139100523	31.467441351486396	20.699694007755106	23.83435950165797
32	29.18922378728377	24.37511097896871	21.72565585989602	24.710009373851495
33	26.908631789331977	25.70262591725408	24.54194075891032	22.84680153450362
34	19.51020593567143	27.90517131023319	22.704954927053265	29.879667827042116
35	19.93295837927346	22.537299257111947	28.01573733394449	29.5140050296701
36	27.809264834016755	22.705161399553194	25.784801972225317	23.70077179420473
37	26.456663486990166	24.272700619004556	24.071183459075087	25.19945243493019
38	21.10334774511383	26.698546020655513	28.308928283841876	23.889177950388788
39	20.445732832843994	27.60186320783935	25.588343388544082	26.36406057077258
40	24.936509706272222	21.522383683717166	27.95193733146682	25.58916927854379
41	20.17174382543989	20.954274600166002	28.6988515999554	30.175129974438704
42	21.513815074970164	23.98694267910457	24.433439460198297	30.06580278572697
43	21.87359340609424	21.26553189380706	28.073756106424185	28.78711859367451
44	27.630149940329446	23.88639057163976	22.991435520702996	25.492023967327793
45	19.860899476798686	26.236770274567128	26.111131758361104	27.79119849027308
46	22.375528053418563	24.9322770200237	24.547825225158263	28.14436970139947
47	23.497293145525948	22.472983073384455	29.32477298348633	24.704950797603267
48	21.989734187303593	20.165239941692167	33.635299364477646	24.209726506526597
49	21.469836432485558	18.5462890697588	27.814529882764916	32.16934461499073
50	20.85650987145022	21.941419622320506	31.812869843865492	25.38920066236378
51	22.24937335596272	23.75548700668558	27.150617559247287	26.844522078104415
52	19.786775849324627	20.977709228907802	28.714233801200017	30.52128112056755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	518.0
1	429.0
2	340.0
3	279.0
4	218.0
5	273.5
6	329.0
7	409.5
8	490.0
9	566.0
10	642.0
11	685.0
12	728.0
13	778.5
14	872.0
15	915.0
16	1057.5
17	1200.0
18	1326.0
19	1452.0
20	1304.0
21	1156.0
22	1257.0
23	1358.0
24	1938.0
25	2518.0
26	4250.0
27	5982.0
28	5493.5
29	5005.0
30	6666.5
31	8328.0
32	9222.0
33	10116.0
34	10787.0
35	11458.0
36	13760.5
37	16063.0
38	19355.0
39	33303.5
40	43960.0
41	48730.0
42	53500.0
43	58752.0
44	64004.0
45	71444.5
46	78885.0
47	86480.5
48	94076.0
49	107261.5
50	120447.0
51	120872.0
52	121297.0
53	102715.5
54	84134.0
55	69600.5
56	55067.0
57	48446.5
58	41826.0
59	38897.0
60	35968.0
61	30552.0
62	25136.0
63	21869.5
64	16674.0
65	14745.0
66	11496.0
67	8247.0
68	7285.5
69	6324.0
70	5643.0
71	4962.0
72	4256.5
73	3551.0
74	2170.0
75	789.0
76	636.5
77	484.0
78	297.0
79	110.0
80	138.0
81	166.0
82	100.0
83	34.0
84	25.5
85	17.0
86	19.0
87	21.0
88	12.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	968652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.126039890482227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.32360543777993	9.880874616419808
2	12.075190853074247	3.652996370575743
3	5.841555490634523	2.6507880412140974
4	3.4954187276914483	2.114873724359979
5	2.370232892256152	1.7926118638999808
6	1.6358806725699842	1.4846637785617482
7	1.2402525636456163	1.3132076826383507
8	0.9249444361338021	1.1192597149951584
9	0.6742101147018408	0.9178316180591978
>10	4.183642164578108	11.95192052147116
>50	0.6191830936986781	6.804911143541505
>100	1.5865286766876825	49.52808461270264
>500	0.017066788690702266	1.7102115077288986
>1k	0.010240073214421358	3.404727317524287
>5k	0.0020480146428842718	1.6730374863074275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	5811	0.5999058485400329	TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	5256	0.5426097298100866	TruSeq Adapter, Index 13 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	5135	0.5301181435644586	TruSeq Adapter, Index 13 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	4223	0.43596668359741164	TruSeq Adapter, Index 8 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3975	0.4103640936063726	TruSeq Adapter, Index 8 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3774	0.38961360736363526	TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3187	0.32901392863484513	TruSeq Adapter, Index 13 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2965	0.3060954811428666	TruSeq Adapter, Index 13 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2476	0.2556129549105355	TruSeq Adapter, Index 8 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2052	0.21184078492585573	TruSeq Adapter, Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1647	0.17003010369048946	TruSeq Adapter, Index 13 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1590	0.16414563744254904	TruSeq Adapter, Index 8 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1452	0.14989903494753534	TruSeq Adapter, Index 8 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	1164	0.12016699495794156	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1158	0.11954757745815835	TruSeq Adapter, Index 13 (95% over 24bp)
GACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1132	0.11686343495909779	TruSeq Adapter, Index 8 (95% over 23bp)
AACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1099	0.11345663871029019	TruSeq Adapter, Index 13 (95% over 23bp)
GTGTGTGTGTGTTTAATGGATATTTTTCAGACAATATATTTTGATTATGTTC	1078	0.11128867746104897	No Hit
TTTTTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCA	980	0.10117152496458998	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	973	0.1004488712148429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6242696035315056	0.0
2	0.0	0.0	0.0	1.5385298332115145	0.0
3	0.0	0.0	0.0	5.294264606897007	0.0
4	0.0	0.0	0.0	6.1023979716141605	0.0
5	0.0	0.0	0.0	7.415666307404517	0.0
6	0.0	0.0	0.0	11.45891403723938	0.0
7	0.0	0.0	0.0	12.009576194546648	0.0
8	0.0	0.0	0.0	13.116784975409125	0.0
9	0.0	0.0	0.0	13.809706685166603	0.0
10	0.0	0.0	0.0	15.925636864426027	0.0
11	0.0	0.0	0.0	17.588463142594037	0.0
12	0.0	0.0	0.0	18.3311447248341	0.0
13	0.0	0.0	0.0	18.684419172210454	0.0
14	0.0	0.0	0.0	18.85682370965011	0.0
15	0.0	0.0	0.0	19.131432134554	0.0
16	0.0	0.0	0.0	19.76788361558124	0.0
17	0.0	0.0	0.0	20.67976941151208	0.0
18	0.0	0.0	0.0	21.78439728612546	0.0
19	0.0	0.0	0.0	22.392768507162533	0.0
20	0.0	0.0	0.0	23.057919665679727	0.0
21	1.0323624996386731E-4	0.0	0.0	24.07500320032375	0.0
22	1.0323624996386731E-4	0.0	0.0	24.86063106254878	0.0
23	1.0323624996386731E-4	0.0	0.0	25.761780288483376	0.0
24	3.0970874989160195E-4	0.0	0.0	26.373661542019217	0.0
25	3.0970874989160195E-4	0.0	0.0	26.927730495575293	0.0
26	3.0970874989160195E-4	0.0	0.0	27.429665142899616	0.0
27	3.0970874989160195E-4	0.0	0.0	27.88307875274092	0.0
28	3.0970874989160195E-4	0.0	0.0	28.314193332590033	0.0
29	3.0970874989160195E-4	0.0	0.0	28.78391826992563	0.0
30	3.0970874989160195E-4	0.0	0.0	29.33788398723174	0.0
31	3.0970874989160195E-4	0.0	0.0	29.83651507455722	0.0
32	3.0970874989160195E-4	0.0	0.0	30.296226095646322	0.0
33	3.0970874989160195E-4	0.0	0.0	30.758414786734555	0.0
34	3.0970874989160195E-4	0.0	0.0	31.203672732828714	0.0
35	3.0970874989160195E-4	0.0	0.0	31.771472107629986	0.0
36	3.0970874989160195E-4	0.0	0.0	32.25740513620991	0.0
37	3.0970874989160195E-4	0.0	0.0	32.7237232772967	0.0
38	3.0970874989160195E-4	0.0	0.0	33.20377183962868	0.0
39	3.0970874989160195E-4	0.0	0.0	33.66358609696775	0.0
40	3.0970874989160195E-4	0.0	0.0	34.112870256810496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTT	35	1.0193435E-7	46.000004	1
AACGTGC	35	1.0193435E-7	46.000004	40
TGTCAGT	35	1.0193435E-7	46.000004	20
CAGGTAG	35	1.0193435E-7	46.000004	11
AGGTAGT	35	1.0193435E-7	46.000004	14
TCGTTGA	85	0.0	46.000004	24
AATCCCC	35	1.0193435E-7	46.000004	45
AAATCTC	35	1.0193435E-7	46.000004	44
TGCCCGC	35	1.0193435E-7	46.000004	27
ACCGGAC	35	1.0193435E-7	46.000004	25
GTCGATA	35	1.0193435E-7	46.000004	8
CGTGCCG	35	1.0193435E-7	46.000004	30
CAACCGT	35	1.0193435E-7	46.000004	37
TTGTACG	35	1.0193435E-7	46.000004	1
TACGGTA	35	1.0193435E-7	46.000004	37
GGTCAAC	35	1.0193435E-7	46.000004	8
CGACATA	35	1.0193435E-7	46.000004	29
TCGATTC	35	1.0193435E-7	46.000004	38
TAACGAT	35	1.0193435E-7	46.000004	15
TCGATCC	35	1.0193435E-7	46.000004	12
>>END_MODULE