SRR1545576_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545576_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2226422
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	6303	0.2830999693678916	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5839	0.2622593560430143	No Hit
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	4477	0.20108496951611152	Illumina Single End Adapter 1 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	3219	0.14458175494133635	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2991	0.13434110873859492	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	2845	0.12778350195964647	Illumina Single End Adapter 1 (95% over 23bp)
ATGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	2424	0.10887423857651425	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	2381	0.10694288863476915	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	40	6.8193913E-9	45.000004	2
TATTCGA	20	7.0347113E-4	45.000004	15
CAAATCG	40	6.8193913E-9	45.000004	15
TCCGTAT	20	7.0347113E-4	45.000004	39
TCGTAGT	75	0.0	45.000004	2
CGCACAT	20	7.0347113E-4	45.000004	14
ATACGTT	150	0.0	45.000004	1
CTTACGA	20	7.0347113E-4	45.000004	29
AATCCCG	35	1.2126111E-7	45.0	34
ATTACGC	110	0.0	45.0	1
CCCGTAA	25	3.8919876E-5	45.0	28
TCGCTCA	55	1.8189894E-12	45.0	32
ACGTGCA	45	3.8562575E-10	45.0	23
ATACCCG	35	1.2126111E-7	45.0	1
TACCGAA	25	3.8919876E-5	45.0	30
TAATGCG	100	0.0	42.75	3
TTCGTTA	85	0.0	42.35294	2
CGTCCTA	80	0.0	42.187504	14
CAGTACG	55	6.184564E-11	40.909092	27
ACACGTT	50	1.0822987E-9	40.5	25