##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545576_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2226422
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89741253005944	31.0	31.0	34.0	31.0	34.0
2	32.55181138166978	34.0	31.0	34.0	31.0	34.0
3	32.526555163396694	34.0	31.0	34.0	31.0	34.0
4	36.00634291252961	37.0	35.0	37.0	35.0	37.0
5	35.786134883683324	37.0	35.0	37.0	35.0	37.0
6	35.79345020845105	37.0	35.0	37.0	35.0	37.0
7	35.77465772436672	37.0	35.0	37.0	35.0	37.0
8	35.750372121727146	37.0	35.0	37.0	33.0	37.0
9	37.91510998364191	39.0	38.0	39.0	35.0	39.0
10	37.86095807533343	39.0	38.0	39.0	35.0	39.0
11	37.685923423322265	39.0	38.0	39.0	35.0	39.0
12	37.21012817875497	39.0	37.0	39.0	33.0	39.0
13	36.98613874638321	39.0	37.0	39.0	33.0	39.0
14	37.80444183537532	40.0	36.0	41.0	33.0	41.0
15	37.766621961155614	40.0	36.0	41.0	33.0	41.0
16	37.801604996716705	40.0	36.0	41.0	33.0	41.0
17	37.84947058554039	40.0	36.0	41.0	33.0	41.0
18	37.86384881212996	40.0	36.0	41.0	33.0	41.0
19	37.810463604833224	40.0	36.0	41.0	33.0	41.0
20	37.76555522717616	40.0	36.0	41.0	33.0	41.0
21	37.74957712419299	40.0	36.0	41.0	33.0	41.0
22	37.682450586636314	40.0	35.0	41.0	33.0	41.0
23	37.634939827220535	40.0	35.0	41.0	33.0	41.0
24	37.58000415015662	40.0	35.0	41.0	32.0	41.0
25	37.48379238077956	40.0	35.0	41.0	32.0	41.0
26	37.38820942301145	40.0	35.0	41.0	32.0	41.0
27	37.28099973859403	39.0	35.0	41.0	32.0	41.0
28	37.17716003524938	39.0	35.0	41.0	32.0	41.0
29	37.098609338211716	39.0	35.0	41.0	31.0	41.0
30	36.98100494874736	39.0	35.0	41.0	31.0	41.0
31	36.90640498521844	39.0	35.0	41.0	31.0	41.0
32	36.77843104317151	39.0	35.0	41.0	31.0	41.0
33	36.65903094741248	39.0	35.0	41.0	30.0	41.0
34	36.61184133106841	39.0	35.0	41.0	30.0	41.0
35	36.503315633783714	39.0	35.0	41.0	30.0	41.0
36	36.41940341947753	39.0	35.0	41.0	30.0	41.0
37	36.268710513999594	38.0	35.0	41.0	30.0	41.0
38	36.18099309115702	38.0	35.0	41.0	30.0	41.0
39	36.111720060258115	38.0	35.0	41.0	30.0	41.0
40	36.05099078252012	38.0	35.0	40.0	30.0	41.0
41	35.96874626643107	38.0	35.0	40.0	30.0	41.0
42	35.849257687895644	38.0	35.0	40.0	29.0	41.0
43	35.708902445268684	38.0	35.0	40.0	29.0	41.0
44	35.59830705948827	38.0	34.0	40.0	29.0	41.0
45	35.55143858621591	37.0	34.0	40.0	29.0	41.0
46	35.47554506737716	37.0	34.0	40.0	28.0	41.0
47	35.38138681705445	37.0	34.0	40.0	28.0	41.0
48	35.304533013058624	37.0	34.0	40.0	28.0	41.0
49	35.2298117787194	37.0	34.0	40.0	28.0	41.0
50	35.10302943467141	36.0	34.0	40.0	28.0	41.0
51	34.91282290598997	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	22.0
11	23.0
12	30.0
13	35.0
14	37.0
15	80.0
16	160.0
17	295.0
18	673.0
19	1136.0
20	2067.0
21	3385.0
22	5235.0
23	7839.0
24	11143.0
25	15764.0
26	21303.0
27	26185.0
28	30946.0
29	36990.0
30	44257.0
31	54552.0
32	67308.0
33	87390.0
34	137574.0
35	233734.0
36	174415.0
37	243267.0
38	361436.0
39	658843.0
40	284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.2511455600061443	90.70894915698821	3.413144498212828	3.62676078479282
2	3.2615559853433	2.800726906219935	91.10635809383845	2.8313590145983105
3	28.32459434913956	15.72181733741402	42.000977352900755	13.952610960545664
4	27.401678567674953	15.695003013804213	43.55441151767275	13.348906900848087
5	25.08567558171811	14.089197825030475	45.805197756759505	15.019928836491914
6	23.783541484947598	14.747518664476006	46.30883094040573	15.160108910170669
7	23.028159082150644	17.09747747731562	43.855073296976045	16.01929014355769
8	25.72427868571187	19.820052083567266	42.55967646744418	11.895992763276682
9	82.3555462531362	4.360583932426108	9.06292697431125	4.220942840126445
10	83.29629333522576	4.380616073682347	8.478895734950518	3.8441948561413786
11	76.68860620313669	6.754424812546768	9.98269869773116	6.5742702865853815
12	42.22815800418789	22.951983047239022	16.078802670832392	18.741056277740697
13	33.11380322328831	24.457582614616634	23.2712845992359	19.15732956285915
14	28.26274623588879	22.572180835439106	27.72717840553139	21.437894523140717
15	22.47049301525048	23.913705487998232	31.49340062216417	22.122400874587118
16	19.662085624378488	29.569461674381586	29.608537824365733	21.159914876874193
17	19.710593948496737	22.758443817030194	36.61390338399459	20.917058850478483
18	23.308159908588756	20.433143402284024	33.849198399943944	22.409498289183272
19	22.867587546296257	20.825387100917975	29.092418238770545	27.214607114015223
20	23.842335370383513	21.390509076895576	31.48360912711067	23.28354642561024
21	26.50544236447538	23.991902703081447	26.785398275798567	22.717256656644608
22	28.148482183521363	21.64005745541501	26.351248774940238	23.860211586123388
23	27.074966021715557	23.631593651158674	27.005751829617207	22.287688497508558
24	29.220695806994364	20.44001541486744	25.09173912223289	25.247549655905306
25	28.85850032024477	22.581972330492604	23.677676559071013	24.881850790191617
26	27.777079098212287	20.30297939923339	28.44653888615905	23.473402616395276
27	28.746482023623553	21.800539161039552	25.555263108251715	23.89771570708518
28	25.83234445221975	24.849242416756574	24.788112945344594	24.530300185679085
29	25.312227421396305	23.975912922168394	25.57430711697962	25.137552539455683
30	24.623364303802244	24.472808838575975	28.054699423559416	22.849127434062368
31	24.95995817504498	23.76593475989727	26.622356408623343	24.651750656434405
32	26.29155658720584	23.828546430101753	27.039483080925358	22.84041390176705
33	26.823710868828996	25.608981585701184	24.294181426522016	23.2731261189478
34	28.758564189538195	24.833611956762912	24.29440600209664	22.113417851602257
35	27.579183101855804	24.174797051053215	24.642677803219694	23.603342043871287
36	26.35937841074154	24.645462540345	24.97141152935068	24.023747519562775
37	25.64711451827192	23.679158757863515	27.227542667113426	23.446184056751147
38	27.65544896699727	25.67289579423847	24.258788315961663	22.412866922802596
39	26.049239542189216	27.112065906643036	25.435564326978444	21.403130224189304
40	26.469734848110555	24.955601408897323	25.56105715807695	23.01360658491517
41	24.99373434146806	25.648237396145024	24.441278427899114	24.9167498344878
42	26.821959179346955	22.977315172056333	27.139509041861785	23.061216606734934
43	23.794590603218975	24.566187362503605	27.74972579322339	23.889496241054033
44	24.29431617186679	25.435070260714276	25.09879079527601	25.171822772142928
45	24.46458937254483	22.390229704880745	27.069396547464947	26.075784375109478
46	25.262506389175098	23.92830290034863	26.279249845716578	24.52994086475969
47	23.570688755321317	24.461939380764296	26.815626148142623	25.15174571577176
48	23.949413004363056	24.61851347139042	25.702180449169116	25.72989307507741
49	24.64052187770333	24.806528142463556	27.701621705139456	22.851328274693657
50	24.71386826037472	22.847330829465392	29.090801294633273	23.347999615526614
51	23.70008920141824	24.355310897933993	26.130266409512664	25.814333491135105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	876.0
1	1931.0
2	2986.0
3	6679.0
4	10372.0
5	7227.0
6	4082.0
7	4016.0
8	3950.0
9	4094.0
10	4238.0
11	4258.0
12	4278.0
13	4631.0
14	4984.0
15	5125.0
16	5266.0
17	4938.5
18	4611.0
19	5316.0
20	6021.0
21	6988.0
22	7955.0
23	7793.5
24	7632.0
25	9529.5
26	17031.5
27	22636.0
28	23404.0
29	24172.0
30	27446.5
31	30721.0
32	36207.5
33	41694.0
34	45508.5
35	49323.0
36	53878.0
37	58433.0
38	71007.5
39	83582.0
40	98173.0
41	112764.0
42	127717.0
43	142670.0
44	158116.0
45	173562.0
46	184089.5
47	194617.0
48	189476.0
49	184335.0
50	166937.5
51	149540.0
52	139355.5
53	129171.0
54	132377.0
55	135583.0
56	143901.0
57	152219.0
58	140114.0
59	128009.0
60	112514.0
61	97019.0
62	86086.5
63	75154.0
64	63669.5
65	52185.0
66	45299.0
67	38413.0
68	33454.0
69	28495.0
70	23885.5
71	19276.0
72	15666.5
73	12057.0
74	8998.0
75	4355.0
76	2771.0
77	2429.0
78	2087.0
79	1511.0
80	935.0
81	571.5
82	208.0
83	123.0
84	38.0
85	38.5
86	39.0
87	29.5
88	20.0
89	32.0
90	44.0
91	35.0
92	26.0
93	15.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2226422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.945541417625165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98336962548166	11.63759343160964
2	10.850910005538541	3.460472698244762
3	4.259051946235733	2.037386676255568
4	2.2959569946804352	1.464411094070523
5	1.4100311189945598	1.1241854804034055
6	0.9122044703514591	0.872735649799921
7	0.6335956496617817	0.7072117971596344
8	0.4708052091243213	0.6005795169380432
9	0.36077154904385517	0.5177427909821622
>10	2.739013662297416	9.873134828427975
>50	1.2126882995694022	14.379477153554465
>100	1.856619611620626	50.099416242776066
>500	0.009045649751062911	0.9767151607862573
>1k	0.005370854539693603	1.7016452420509844
>5k	5.653531094414319E-4	0.5472922369406403
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	6303	0.2830999693678916	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5839	0.2622593560430143	No Hit
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	4477	0.20108496951611152	Illumina Single End Adapter 1 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	3219	0.14458175494133635	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	2991	0.13434110873859492	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	2845	0.12778350195964647	Illumina Single End Adapter 1 (95% over 23bp)
ATGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	2424	0.10887423857651425	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	2381	0.10694288863476915	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1389673655757983	0.0
2	0.0	0.0	0.0	0.604063380616972	0.0
3	0.0	0.0	0.0	0.8884209732027442	0.0
4	4.4915114924304556E-5	0.0	0.0	1.20902506353243	0.0
5	4.4915114924304556E-5	0.0	0.0	2.2046584160594893	0.0
6	4.4915114924304556E-5	0.0	0.0	2.444415299525427	0.0
7	4.4915114924304556E-5	0.0	0.0	2.850582683785913	0.0
8	4.4915114924304556E-5	0.0	0.0	4.099133048451731	0.0
9	4.4915114924304556E-5	0.0	0.0	4.254584261204749	0.0
10	4.4915114924304556E-5	0.0	0.0	4.5695290470539724	0.0
11	4.4915114924304556E-5	0.0	0.0	4.801066464488763	0.0
12	4.4915114924304556E-5	0.0	0.0	5.5256370984476435	0.0
13	8.983022984860911E-5	0.0	0.0	6.115731878323157	0.0
14	1.3474534477291367E-4	0.0	0.0	6.366043813796306	0.0
15	1.3474534477291367E-4	0.0	0.0	6.49661205288126	0.0
16	1.3474534477291367E-4	0.0	0.0	6.590888879107375	0.0
17	1.3474534477291367E-4	0.0	0.0	6.715663068367093	0.0
18	1.3474534477291367E-4	0.0	0.0	6.972532610619191	0.0
19	1.3474534477291367E-4	0.0	0.0	7.30256887508298	0.0
20	1.7966045969721822E-4	0.0	0.0	7.674780432460692	4.4915114924304556E-5
21	1.7966045969721822E-4	0.0	0.0	7.918355100695196	4.4915114924304556E-5
22	1.7966045969721822E-4	0.0	0.0	8.200466937534753	4.4915114924304556E-5
23	2.2457557462152278E-4	0.0	0.0	8.632235937302093	4.4915114924304556E-5
24	2.2457557462152278E-4	0.0	0.0	8.998922935544115	4.4915114924304556E-5
25	2.6949068954582733E-4	0.0	0.0	9.433297011977064	4.4915114924304556E-5
26	2.6949068954582733E-4	0.0	0.0	9.737237594669834	4.4915114924304556E-5
27	3.144058044701319E-4	0.0	0.0	10.014229108408019	4.4915114924304556E-5
28	3.5932091939443645E-4	0.0	0.0	10.272491019222771	4.4915114924304556E-5
29	3.5932091939443645E-4	0.0	0.0	10.516200432802048	4.4915114924304556E-5
30	3.5932091939443645E-4	0.0	0.0	10.774103022697405	4.4915114924304556E-5
31	4.0423603431874103E-4	0.0	0.0	11.054238594480292	4.4915114924304556E-5
32	4.4915114924304556E-4	0.0	0.0	11.3395843195944	4.4915114924304556E-5
33	4.940662641673501E-4	0.0	0.0	11.635844417635111	4.4915114924304556E-5
34	5.389813790916547E-4	0.0	0.0	11.938662122454772	4.4915114924304556E-5
35	5.389813790916547E-4	0.0	0.0	12.234248493771622	4.4915114924304556E-5
36	5.389813790916547E-4	0.0	0.0	12.529789949973544	4.4915114924304556E-5
37	5.838964940159592E-4	0.0	0.0	12.843342367260115	4.4915114924304556E-5
38	5.838964940159592E-4	0.0	0.0	13.152852424203498	4.4915114924304556E-5
39	5.838964940159592E-4	0.0	0.0	13.46716839844378	4.4915114924304556E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	40	6.8193913E-9	45.000004	2
TATTCGA	20	7.0347113E-4	45.000004	15
CAAATCG	40	6.8193913E-9	45.000004	15
TCCGTAT	20	7.0347113E-4	45.000004	39
TCGTAGT	75	0.0	45.000004	2
CGCACAT	20	7.0347113E-4	45.000004	14
ATACGTT	150	0.0	45.000004	1
CTTACGA	20	7.0347113E-4	45.000004	29
AATCCCG	35	1.2126111E-7	45.0	34
ATTACGC	110	0.0	45.0	1
CCCGTAA	25	3.8919876E-5	45.0	28
TCGCTCA	55	1.8189894E-12	45.0	32
ACGTGCA	45	3.8562575E-10	45.0	23
ATACCCG	35	1.2126111E-7	45.0	1
TACCGAA	25	3.8919876E-5	45.0	30
TAATGCG	100	0.0	42.75	3
TTCGTTA	85	0.0	42.35294	2
CGTCCTA	80	0.0	42.187504	14
CAGTACG	55	6.184564E-11	40.909092	27
ACACGTT	50	1.0822987E-9	40.5	25
>>END_MODULE