FastQCFastQC Report
Thu 26 May 2016
SRR1545575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545575_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1821726
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT77760.4268479453002263Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT48980.2688658997017115Illumina Single End Adapter 1 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC45530.24992781570883876Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC38900.2135337586442747Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC38380.21067932279607363Illumina Single End Adapter 1 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC29720.16314198732410912Illumina Single End Adapter 1 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC29370.16122073242628146Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC28410.15595100470652556Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT23850.13091979803768514Illumina Single End Adapter 1 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC22720.12471688936755582Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC19820.1087979202141266Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG351.0200165E-746.00000439
TAATGCG351.0200165E-746.0000041
TCAAACG351.0200165E-746.00000435
AACCGTG253.4179495E-546.034
ACACGTT301.8620067E-646.023
CGTGATA301.8620067E-646.042
CGGCATA253.4179495E-546.018
ATTACCG850.046.016
TTATACG253.4179495E-546.01
CTAACGT206.312433E-446.045
TGCGATA206.312433E-446.014
TCGCTTA206.312433E-446.012
TCGCTAT253.4179495E-546.039
TCGTAAA253.4179495E-546.020
TCATCGG253.4179495E-546.017
TTACGCA206.312433E-446.027
CGGTCTA206.312433E-446.028
ACGTAAG301.8620067E-646.044
TCGACTA206.312433E-446.041
TCGACAA301.8620067E-646.019
Produced by FastQC (version 0.11.5)