FastQCFastQC Report
Thu 26 May 2016
SRR1545574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545574_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2023975
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.23913338850529278No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC45070.2226806161143295No Hit
ATGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT24040.11877617065428181No Hit
ATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG21600.10672068577922157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA551.8189894E-1245.019
CGACTTA207.034562E-445.032
ATACGCA603.6379788E-1241.2500041
ATCGTTC1250.039.61
TCGTATT1050.038.571432
ATCGTAC700.038.5714261
CGAATTA700.038.57142618
ATTGTTT37500.038.4599951
ATCGTTA1600.037.968751
ATCGGTT2500.037.81
CGTTTTT28750.037.5652161
CGACATA301.14022885E-437.5000048
CGGGATA2850.037.105268
ACATACG4800.037.03125419
AGTTACG1650.036.8181843
TTACGGG7700.036.5259745
AGGGTAC3150.036.4285748
ATGCGAT1800.036.251
ATTCGTT4600.036.1956521
ATGCGTT4850.036.185571
Produced by FastQC (version 0.11.5)