##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545574_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2023975
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81681246062822	31.0	31.0	34.0	31.0	34.0
2	32.454619251720004	33.0	31.0	34.0	31.0	34.0
3	32.4469373386529	34.0	31.0	34.0	31.0	34.0
4	35.95500339678109	37.0	35.0	37.0	35.0	37.0
5	35.73273829963315	37.0	35.0	37.0	35.0	37.0
6	35.74678392766709	37.0	35.0	37.0	35.0	37.0
7	35.73613409256537	37.0	35.0	37.0	33.0	37.0
8	35.71626675230672	37.0	35.0	37.0	33.0	37.0
9	37.86032040909596	39.0	38.0	39.0	35.0	39.0
10	37.784438048888944	39.0	38.0	39.0	35.0	39.0
11	37.61675317135834	39.0	37.0	39.0	35.0	39.0
12	37.15466890648353	39.0	37.0	39.0	33.0	39.0
13	36.937302585259204	39.0	37.0	39.0	33.0	39.0
14	37.7873842315246	40.0	36.0	41.0	33.0	41.0
15	37.756628910930225	40.0	36.0	41.0	33.0	41.0
16	37.678322113662475	40.0	36.0	41.0	32.0	41.0
17	37.76234489062365	40.0	36.0	41.0	32.0	41.0
18	37.81817561975815	40.0	36.0	41.0	33.0	41.0
19	37.782449387961805	40.0	36.0	41.0	33.0	41.0
20	37.72179350041379	40.0	36.0	41.0	33.0	41.0
21	37.69320421447893	40.0	36.0	41.0	33.0	41.0
22	37.60786719203548	40.0	36.0	41.0	32.0	41.0
23	37.55110710359565	40.0	35.0	41.0	32.0	41.0
24	37.53710495435961	40.0	35.0	41.0	32.0	41.0
25	37.46602057831742	40.0	35.0	41.0	32.0	41.0
26	37.33988463296237	39.0	35.0	41.0	32.0	41.0
27	37.249044578119786	39.0	35.0	41.0	32.0	41.0
28	37.157812720018775	39.0	35.0	41.0	31.0	41.0
29	37.1133047591991	39.0	35.0	41.0	31.0	41.0
30	37.04014575278845	39.0	35.0	41.0	31.0	41.0
31	36.93099321878976	39.0	35.0	41.0	31.0	41.0
32	36.776604948183646	39.0	35.0	41.0	31.0	41.0
33	36.673925814301064	39.0	35.0	41.0	30.0	41.0
34	36.60605343445447	39.0	35.0	41.0	30.0	41.0
35	36.48884546498845	39.0	35.0	41.0	30.0	41.0
36	36.378778394001905	39.0	35.0	41.0	30.0	41.0
37	36.26415099000729	39.0	35.0	41.0	30.0	41.0
38	36.16580985437073	38.0	35.0	41.0	30.0	41.0
39	36.090079669956395	38.0	35.0	41.0	29.0	41.0
40	36.00175891500636	38.0	35.0	40.0	29.0	41.0
41	35.898788275546885	38.0	35.0	40.0	29.0	41.0
42	35.76930446275275	38.0	35.0	40.0	28.0	41.0
43	35.62509615978458	38.0	34.0	40.0	28.0	41.0
44	35.55367926975383	38.0	34.0	40.0	28.0	41.0
45	35.51984782420732	38.0	34.0	40.0	28.0	41.0
46	35.43601576106424	37.0	34.0	40.0	28.0	41.0
47	35.34727257006633	37.0	34.0	40.0	28.0	41.0
48	35.27444459541249	37.0	34.0	40.0	28.0	41.0
49	35.22401462468657	37.0	34.0	40.0	28.0	41.0
50	35.09198581998295	37.0	34.0	40.0	27.0	41.0
51	34.86893019923665	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	26.0
11	24.0
12	37.0
13	39.0
14	46.0
15	76.0
16	177.0
17	354.0
18	667.0
19	1296.0
20	2182.0
21	3625.0
22	5566.0
23	8163.0
24	11319.0
25	15529.0
26	20212.0
27	24828.0
28	29046.0
29	34140.0
30	41255.0
31	50616.0
32	61914.0
33	80587.0
34	127957.0
35	187306.0
36	159118.0
37	222823.0
38	333081.0
39	601681.0
40	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.407391395644709	90.39775688928964	3.5460912313640236	3.648760483701627
2	3.7558270235551325	3.083289072246446	89.97492557961438	3.1859583245840484
3	29.439247026272557	16.510628836818636	40.69837819143023	13.351745945478577
4	28.35944119863141	15.503106510702949	41.901604515866055	14.23584777479959
5	25.47738978989365	14.061092651836116	45.548783952370954	14.91273360589928
6	23.551377857928088	14.869057177089637	46.19849553477686	15.381069430205413
7	23.220247285663113	17.424721155152607	43.9016786274534	15.453352931730876
8	26.335750194542918	20.01200607715016	42.210748650551515	11.441495077755407
9	81.76963648266407	4.631331908743932	9.112266702898998	4.486764905693005
10	82.28821996319124	4.623327857310491	9.068936128163637	4.019516051334627
11	75.81679615607901	7.236205980805099	10.396669919341889	6.550327943774009
12	41.97739596585926	23.304339233439148	17.01261132178016	17.70565347892143
13	32.69976160772737	24.901987425734013	23.978062970145384	18.420187996393235
14	26.386541335737846	23.34697810002594	29.16177324324658	21.10470732098964
15	23.0306204375054	23.699996294420632	33.087266394100716	20.182116873973246
16	20.01166022307588	28.03399251472968	31.53280055336652	20.421546708827925
17	20.199804839486653	22.966835064662362	36.084338986400525	20.749021109450464
18	25.144282908632764	21.143195938685015	31.96492051532257	21.74760063735965
19	24.446991687150284	21.724329598932794	28.92194814659272	24.9067305673242
20	24.850010499141543	21.85061574377154	30.397707481564744	22.901666275522178
21	27.313084400746057	24.01808322731259	26.860410825232524	21.80842154670883
22	26.422411344013636	23.249743697427093	27.387986511691103	22.939858446868165
23	26.714559221334255	23.842142318951566	27.838634370483824	21.60466408923035
24	29.645919539520005	20.4713002878	26.469398090391433	23.413382082288567
25	28.32455934485357	23.24623574895935	24.93484356279104	23.49436134339604
26	26.98017515038476	22.1173186427698	28.484739188972195	22.417767017873246
27	28.332711619461705	22.925629022097603	24.865919786558628	23.87573957188206
28	24.676095307501328	24.94966588026038	25.404019318420435	24.970219493817858
29	24.121938265047742	23.33912227176719	27.414716090860807	25.124223372324263
30	23.73245716967848	23.395545893600463	27.839869563606268	25.032127373114786
31	24.663842191726676	23.17899183537346	27.287837053323287	24.869328919576574
32	25.86958831013229	24.089675020689487	27.33734359367087	22.703393075507357
33	25.659457256141998	24.40450104373819	25.14749441075112	24.788547289368694
34	27.59668474165936	23.622920243580083	25.716424362949148	23.06397065181141
35	26.035795896688445	24.89161180350548	25.1903803159624	23.882211983843675
36	24.296792203461013	24.72604651737299	26.139799157598294	24.837362121567708
37	24.444175446831114	24.73686680912561	27.87405970923554	22.944898034807743
38	25.99246532195309	26.470435652614288	24.33864054644944	23.198458478983188
39	25.5991304240418	26.79227757259848	25.45145073432231	22.157141269037414
40	24.963450635506863	24.719574105411382	26.872960387356564	23.444014871725194
41	23.945404463987945	25.830655022912836	24.73592806235255	25.488012450746673
42	25.780950851665658	23.985128274805767	27.414172605886932	22.819748267641646
43	23.3813658765548	24.717696611865264	27.68779258637088	24.213144925209058
44	23.119257895972034	25.081979767536655	26.586593213848985	25.212169122642326
45	23.07834829975667	23.277609654269448	27.736014525871123	25.908027520102767
46	24.859348559147225	23.495102459269507	27.177311972726937	24.468237008856335
47	22.759866105065527	24.319569164638892	27.28457614348003	25.63598858681555
48	22.68985535888536	24.535036252918143	26.5214244247088	26.25368396348769
49	23.29332131078694	24.570461591669858	28.810731357847803	23.325485739695402
50	23.42627749848689	24.618831754344793	28.81863659383145	23.136254153336875
51	23.338430563618623	24.225496856433505	27.149495423609483	25.286577156338392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	795.0
1	1405.0
2	2015.0
3	5234.5
4	8454.0
5	6016.0
6	3578.0
7	3627.0
8	3676.0
9	4079.0
10	4482.0
11	4530.0
12	4578.0
13	4821.0
14	5064.0
15	5223.5
16	5383.0
17	5695.5
18	6008.0
19	5952.5
20	5897.0
21	7569.0
22	9241.0
23	9598.0
24	9955.0
25	12088.5
26	16151.0
27	18080.0
28	20928.5
29	23777.0
30	28049.0
31	32321.0
32	38591.5
33	44862.0
34	51987.5
35	59113.0
36	63987.0
37	68861.0
38	77142.0
39	85423.0
40	91900.5
41	98378.0
42	107218.0
43	116058.0
44	129173.0
45	142288.0
46	156489.0
47	170690.0
48	167796.0
49	164902.0
50	152488.5
51	140075.0
52	134439.0
53	128803.0
54	128488.0
55	128173.0
56	124085.0
57	119997.0
58	113355.5
59	106714.0
60	98444.5
61	90175.0
62	78621.5
63	67068.0
64	56832.0
65	46596.0
66	39538.5
67	32481.0
68	27098.5
69	21716.0
70	17656.5
71	13597.0
72	11476.5
73	9356.0
74	7149.5
75	4018.5
76	3094.0
77	2399.0
78	1704.0
79	1217.5
80	731.0
81	590.5
82	450.0
83	276.0
84	102.0
85	89.0
86	76.0
87	43.0
88	10.0
89	7.0
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2023975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.453635599954296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41767524483926	13.732766611640033
2	9.39753840514964	3.468374985304142
3	3.60229403318734	1.9942626423698655
4	1.8787369891947026	1.3867811114701727
5	1.163504475744927	1.0735443807156373
6	0.8216511368381036	0.9097470401699128
7	0.5945949494891205	0.7680706969211822
8	0.4629737018115524	0.6834838388473832
9	0.361367310125174	0.6001686592907072
>10	4.15675465368464	19.329727300687807
>50	2.0857958439224453	27.77302510123703
>100	1.0506674434896004	26.377860063024954
>500	0.0021486041745166014	0.2816605510883158
>1k	0.004297208349033203	1.6205270172329158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4840	0.23913338850529278	No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	4507	0.2226806161143295	No Hit
ATGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	2404	0.11877617065428181	No Hit
ATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	2160	0.10672068577922157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.940772489778777E-5	0.0	0.0	0.08137452290665645	0.0
2	4.940772489778777E-5	0.0	0.0	0.4171988290369199	0.0
3	4.940772489778777E-5	0.0	0.0	0.5784656431032992	0.0
4	4.940772489778777E-5	0.0	0.0	0.8077668943539322	0.0
5	4.940772489778777E-5	0.0	0.0	1.6911276078014799	0.0
6	4.940772489778777E-5	0.0	0.0	1.9103990908978619	0.0
7	9.881544979557554E-5	0.0	0.0	2.2969651304981533	0.0
8	9.881544979557554E-5	0.0	0.0	3.3899628206870145	0.0
9	9.881544979557554E-5	0.0	0.0	3.542336244271792	0.0
10	9.881544979557554E-5	0.0	0.0	3.823812053014489	0.0
11	9.881544979557554E-5	0.0	0.0	4.044812806482294	0.0
12	9.881544979557554E-5	0.0	0.0	4.646253041663064	0.0
13	9.881544979557554E-5	0.0	0.0	5.1801035091836605	0.0
14	9.881544979557554E-5	0.0	0.0	5.387912400103756	0.0
15	9.881544979557554E-5	0.0	0.0	5.512321051396386	0.0
16	9.881544979557554E-5	0.0	0.0	5.610741239392779	0.0
17	1.482231746933633E-4	0.0	0.0	5.722106251312392	0.0
18	1.482231746933633E-4	0.0	0.0	5.9498264553662965	0.0
19	1.482231746933633E-4	0.0	0.0	6.2326856804061315	0.0
20	1.482231746933633E-4	0.0	0.0	6.538321866623846	0.0
21	1.482231746933633E-4	0.0	0.0	6.746130757543942	0.0
22	1.482231746933633E-4	0.0	0.0	6.979384626786398	0.0
23	1.482231746933633E-4	0.0	0.0	7.335416692399857	0.0
24	5.928926987734532E-4	0.0	0.0	7.660667745401994	0.0
25	5.928926987734532E-4	0.0	0.0	8.003705579367335	0.0
26	6.42300423671241E-4	0.0	0.0	8.271001371064365	0.0
27	6.917081485690288E-4	0.0	0.0	8.515273162959028	0.0
28	6.917081485690288E-4	0.0	0.0	8.74709420817945	0.0
29	7.411158734668165E-4	0.0	0.0	8.993786978594104	0.0
30	8.893390481601799E-4	0.0	0.0	9.224965723390852	0.0
31	0.0010375622228535432	0.0	0.0	9.481688261959757	0.0
32	0.0010375622228535432	0.0	0.0	9.752689633024124	0.0
33	0.0010375622228535432	0.0	0.0	10.009807433392211	0.0
34	0.0010375622228535432	0.0	0.0	10.280462950382292	0.0
35	0.0010869699477513309	0.0	0.0	10.551266690547067	0.0
36	0.0011363776726491187	0.0	0.0	10.836250447757507	0.0
37	0.0011363776726491187	0.0	0.0	11.156560728269865	0.0
38	0.001284600847342482	0.0	0.0	11.42133672599711	0.0
39	0.0013340085722402697	0.0	0.0	11.70785212267938	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGAA	55	1.8189894E-12	45.0	19
CGACTTA	20	7.034562E-4	45.0	32
ATACGCA	60	3.6379788E-12	41.250004	1
ATCGTTC	125	0.0	39.6	1
TCGTATT	105	0.0	38.57143	2
ATCGTAC	70	0.0	38.571426	1
CGAATTA	70	0.0	38.571426	18
ATTGTTT	3750	0.0	38.459995	1
ATCGTTA	160	0.0	37.96875	1
ATCGGTT	250	0.0	37.8	1
CGTTTTT	2875	0.0	37.565216	1
CGACATA	30	1.14022885E-4	37.500004	8
CGGGATA	285	0.0	37.10526	8
ACATACG	480	0.0	37.031254	19
AGTTACG	165	0.0	36.818184	3
TTACGGG	770	0.0	36.525974	5
AGGGTAC	315	0.0	36.428574	8
ATGCGAT	180	0.0	36.25	1
ATTCGTT	460	0.0	36.195652	1
ATGCGTT	485	0.0	36.18557	1
>>END_MODULE