FastQCFastQC Report
Thu 26 May 2016
SRR1545573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545573_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1700824
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC39600.23282832321274866No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT38870.22853628594140252Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT37770.22206883251882614No Hit
TGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC35360.2078992300202725No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC31980.1880265095036288No Hit
ATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC26680.1568651430130337No Hit
GGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC24940.1466348075991402No Hit
TTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC24600.14463577654125295No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC23160.13616929206078937No Hit
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC17340.10195058395224904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA351.0199619E-746.00000438
TAGCGTA253.417853E-546.030
CGATTGT206.312313E-446.045
TAACGAC206.312313E-446.015
CTAACGC253.417853E-546.016
GTATACG206.312313E-446.01
CGTTACC301.8619339E-645.99999624
TCGTAGA301.8619339E-645.99999617
ACGGGTA1100.041.818185
GTATTCG402.9116745E-740.251
ACGTACA402.9116745E-740.2512
TATACGG402.9116745E-740.252
ACGTCAT355.3746317E-639.42857435
ATGGGCG11500.039.45
CGGGTAT2700.039.185196
TCGATGG1650.039.0303042
ATACGAA4450.038.76404619
CATACGA4500.038.33333218
AACGTCG301.0017684E-438.3333315
CGTACGA301.0017684E-438.3333333