FastQCFastQC Report
Thu 26 May 2016
SRR1545571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545571_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2549684
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT76730.30093925364868745Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC63680.24975644040594835No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC61510.24124558180543157No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT58190.22822436035210636No Hit
TGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC50300.19727934912718595No Hit
ATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC39460.15476427667114825No Hit
TTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC39260.15397986574022507No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC34260.13436959246714494No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC32230.12640782151827443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG351.0202348E-746.00000411
GATCGAC501.6370905E-1146.09
CGCATAT206.312909E-446.043
TTAACGC301.8623032E-646.022
CGTAAAC405.6152203E-946.035
ATAACGG301.8623032E-646.02
ACCGTAG253.418335E-546.029
CGGTAAC253.418335E-546.046
ACGGGTA950.043.578955
ATCCGAT601.8189894E-1242.16666834
AGTACGA554.7293724E-1141.8181836
CGTTACA451.5897967E-840.88888531
GTATACG451.5897967E-840.8888851
TAAATCG451.5897967E-840.88888538
ACGCAGT402.9124385E-740.2543
GAACGTA402.9124385E-740.251
CGTTATG750.039.8666651
TACGGGA3900.039.5128174
ATTCGAC355.3756885E-639.42857411
TTATCGA355.3756885E-639.42857414