##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545571_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2549684
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50414129750981	33.0	31.0	34.0	31.0	34.0
2	32.91824908498465	34.0	31.0	34.0	31.0	34.0
3	33.000860106585755	34.0	33.0	34.0	31.0	34.0
4	36.392492167656854	37.0	37.0	37.0	35.0	37.0
5	36.37507040088105	37.0	37.0	37.0	35.0	37.0
6	36.37991178514671	37.0	37.0	37.0	35.0	37.0
7	36.447059714066526	37.0	37.0	37.0	35.0	37.0
8	36.471524314385626	37.0	37.0	37.0	35.0	37.0
9	38.2669675928468	39.0	39.0	39.0	37.0	39.0
10	38.039291143529944	39.0	39.0	39.0	35.0	39.0
11	37.85793965056062	39.0	38.0	39.0	35.0	39.0
12	37.488872346533924	39.0	37.0	39.0	35.0	39.0
13	37.4067668777778	39.0	37.0	39.0	35.0	39.0
14	38.51116020651971	40.0	38.0	41.0	34.0	41.0
15	38.644243757265606	40.0	38.0	41.0	34.0	41.0
16	38.755679135139886	41.0	38.0	41.0	35.0	41.0
17	38.72015904716035	40.0	38.0	41.0	35.0	41.0
18	38.70404724663919	40.0	38.0	41.0	35.0	41.0
19	38.67040543063376	40.0	38.0	41.0	35.0	41.0
20	38.54125138644632	40.0	37.0	41.0	34.0	41.0
21	38.525670239919926	40.0	37.0	41.0	34.0	41.0
22	38.68114989936008	40.0	38.0	41.0	35.0	41.0
23	38.69237913404171	40.0	38.0	41.0	35.0	41.0
24	38.63489671661272	40.0	37.0	41.0	35.0	41.0
25	38.55189427395709	40.0	37.0	41.0	35.0	41.0
26	38.43600383420063	40.0	37.0	41.0	35.0	41.0
27	38.36164246236004	40.0	36.0	41.0	35.0	41.0
28	38.2936214840741	40.0	36.0	41.0	34.0	41.0
29	38.219891170827445	40.0	36.0	41.0	34.0	41.0
30	38.144956002390884	40.0	36.0	41.0	34.0	41.0
31	38.04864289064841	40.0	36.0	41.0	34.0	41.0
32	37.99267517072704	40.0	35.0	41.0	34.0	41.0
33	37.91740427441204	40.0	35.0	41.0	34.0	41.0
34	37.73954772434545	40.0	35.0	41.0	33.0	41.0
35	37.675503709479294	40.0	35.0	41.0	33.0	41.0
36	37.60807966791179	40.0	35.0	41.0	33.0	41.0
37	37.51247801688366	40.0	35.0	41.0	33.0	41.0
38	37.357509793370475	40.0	35.0	41.0	33.0	41.0
39	37.25070753865969	39.0	35.0	41.0	33.0	41.0
40	37.12551633849528	39.0	35.0	41.0	33.0	41.0
41	36.95340991275781	39.0	35.0	41.0	32.0	41.0
42	36.88274076316908	39.0	35.0	41.0	32.0	41.0
43	36.79159221299581	39.0	35.0	41.0	32.0	41.0
44	36.67712704790084	38.0	35.0	41.0	32.0	41.0
45	36.61623714938792	38.0	35.0	41.0	32.0	41.0
46	36.60622414385469	38.0	35.0	41.0	32.0	41.0
47	36.575251678247184	38.0	35.0	41.0	32.0	41.0
48	36.42416942648579	38.0	35.0	40.0	32.0	41.0
49	36.34040963507635	38.0	35.0	40.0	31.0	41.0
50	36.21366334024138	37.0	35.0	40.0	31.0	41.0
51	36.04151141866992	37.0	35.0	40.0	31.0	41.0
52	35.63054245153517	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	10.0
11	11.0
12	26.0
13	39.0
14	69.0
15	86.0
16	155.0
17	309.0
18	595.0
19	1023.0
20	1679.0
21	2699.0
22	3668.0
23	4939.0
24	6962.0
25	8996.0
26	11233.0
27	14220.0
28	17572.0
29	22283.0
30	28675.0
31	36676.0
32	47710.0
33	67563.0
34	160788.0
35	253683.0
36	161475.0
37	238345.0
38	398973.0
39	1057820.0
40	1396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.89633225136919	13.525911446281185	45.371622522634176	11.206133779715447
2	27.174779305984586	12.794997340846944	47.85518519157668	12.17503816159179
3	27.467717568137857	14.118337801860935	43.50095933456852	14.91298529543269
4	24.486995251176225	14.348601630633443	45.83618989647344	15.328213221716888
5	24.687765228946017	16.29496047353319	42.60371089123201	16.413563406288777
6	26.621338173671717	18.895282709543615	42.44710324887319	12.036275867911476
7	85.38065893655842	3.3293145346639035	7.044637688435115	4.245388840342567
8	86.12200570737394	3.3222156157390486	7.216619785040028	3.33915889184699
9	78.82251290748187	5.139107434489921	9.322253267463733	6.716126390564478
10	45.51885645436846	20.404214796814035	15.507176575607016	18.569752173210485
11	36.04607472926057	23.515110107762375	23.032893487977336	17.405921674999725
12	30.00764016246719	23.11627636993447	26.225602859020963	20.650480608577375
13	25.946391788158845	22.191338220736373	32.13515870986365	19.72711128124113
14	20.391468119186534	28.08551961733297	32.48194678242481	19.041065481055693
15	20.484107050128564	22.573267903002883	36.380351447473494	20.562273599395063
16	26.592981718518843	21.936796873651794	30.618186410551267	20.852034997278093
17	26.819245051543643	22.194593526099705	26.92659168744048	24.05956973491617
18	26.512108951540664	21.649074944189163	29.20883529096155	22.629980813308627
19	28.18439461517584	24.604264685349243	26.143906460565308	21.067434238909605
20	27.168857003456115	25.280348466711956	24.85107958476423	22.699714945067704
21	27.542981796959936	24.807819321923816	27.8283504936298	19.82084838748645
22	29.34842121611933	20.265648605866453	27.177328641510083	23.20860153650413
23	27.345506345100016	23.24539040916443	24.968505901123432	24.440597344612115
24	27.416260211069293	21.875220615574325	27.10986930145069	23.598649871905693
25	31.044631413147666	22.342180442752905	23.72494003178433	22.888248112315097
26	24.450245599062473	24.995371975507553	24.16570053387008	26.388681891559894
27	24.615717084940723	22.592015324251946	26.528228596171132	26.264038994636195
28	23.970578314802932	24.906106011568493	26.333969229128	24.789346444500573
29	24.592302418652665	25.611997408306287	25.740876124256967	24.054824048784084
30	27.15069789040524	24.282695424217277	26.8630544020357	21.70355228334178
31	24.71933776891568	26.390486036701017	24.129617631047612	24.760558563335692
32	28.861145145829838	25.94894112368435	22.537930190564794	22.651983539921027
33	27.812858377744064	23.580843743773737	23.978736188484532	24.627561689997663
34	25.239637539397037	24.142756514140576	25.72020689622714	24.897399050235244
35	23.93127932716368	27.42080979446865	26.12496293658351	22.522947941784157
36	26.539367231390244	25.670710566485887	23.008537528572166	24.781384673551702
37	26.601061151107352	27.068256301565214	24.452677272948335	21.8780052743791
38	25.667612143308737	23.482086407570506	26.957928904130863	23.89237254498989
39	23.952850627764068	25.721618835902802	23.13823987600032	27.187290660332806
40	27.008366526989224	22.58903456271444	26.658989898356033	23.743609011940304
41	24.223354737292937	22.952609029197344	26.294944785314573	26.529091448195146
42	24.19746917657247	24.625639883216902	25.33768890576244	25.83920203444819
43	22.579503969903723	22.468039176619534	28.011745769279646	26.9407110841971
44	26.640046374374236	21.999510527579105	25.703342061212293	25.65710103683437
45	22.564835485495458	22.911937322428976	26.610317200092247	27.91290999198332
46	22.24303090108421	24.45220662638978	25.583366409327585	27.721396063198423
47	23.37438678675475	24.447264837524965	28.678142075645454	23.500206300074833
48	22.39595181206769	24.373412548378546	30.024504997482044	23.206130642071724
49	23.9673230094396	22.536282927609854	26.209796978762856	27.286597084187687
50	21.357038754606453	23.560841265035197	27.720141005708943	27.36197897464941
51	23.695093195862704	24.33144656357415	25.78794078011236	26.185519460450784
52	22.118427224707062	23.196639269807555	27.11163422604527	27.573299279440118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	323.0
1	276.0
2	229.0
3	210.5
4	192.0
5	296.5
6	401.0
7	557.5
8	714.0
9	942.5
10	1171.0
11	1272.5
12	1374.0
13	1357.5
14	1812.0
15	2283.0
16	2352.5
17	2422.0
18	2603.5
19	2785.0
20	3925.0
21	5065.0
22	5118.0
23	5171.0
24	7702.5
25	10234.0
26	10877.5
27	11521.0
28	15082.0
29	18643.0
30	22700.5
31	26758.0
32	32464.0
33	38170.0
34	43158.0
35	48146.0
36	51717.0
37	55288.0
38	63650.0
39	81717.5
40	91423.0
41	100802.0
42	110181.0
43	124066.5
44	137952.0
45	158276.0
46	178600.0
47	203571.5
48	228543.0
49	240093.5
50	251644.0
51	232334.0
52	213024.0
53	192528.5
54	172033.0
55	161930.5
56	151828.0
57	149251.5
58	146675.0
59	139911.5
60	133148.0
61	124733.5
62	116319.0
63	101185.0
64	77439.5
65	68828.0
66	59936.5
67	51045.0
68	43264.0
69	35483.0
70	31524.5
71	27566.0
72	22424.0
73	17282.0
74	14667.0
75	12052.0
76	9871.5
77	7691.0
78	5892.0
79	4093.0
80	3137.5
81	2182.0
82	1610.5
83	1039.0
84	707.0
85	375.0
86	280.0
87	185.0
88	128.0
89	55.0
90	39.0
91	32.0
92	25.0
93	22.5
94	20.0
95	13.0
96	6.0
97	7.0
98	8.0
99	19.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2549684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.62538950222124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.81416475284252	11.440622922860308
2	12.19444436977582	4.0547477482138365
3	5.164874989276037	2.5760417528098456
4	2.8185649834888276	1.8743896275129412
5	1.6060345462228363	1.3350474942488897
6	1.05607644451807	1.053460934054031
7	0.763525178394962	0.888573243989844
8	0.5495260746290014	0.7308868025867076
9	0.4255676330451993	0.6367704893023316
>10	3.5822884302154683	13.03423797621882
>50	1.3240481655448757	16.235721713077996
>100	1.6914113921685032	43.241471011730205
>500	0.005210171932840659	0.561147479687729
>1k	0.003078737960314935	1.1146959231995017
>5k	0.0011841299847365136	1.2221848805070725
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	7673	0.30093925364868745	Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	6368	0.24975644040594835	No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	6151	0.24124558180543157	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	5819	0.22822436035210636	No Hit
TGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	5030	0.19727934912718595	No Hit
ATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	3946	0.15476427667114825	No Hit
TTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	3926	0.15397986574022507	No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	3426	0.13436959246714494	No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	3223	0.12640782151827443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3209809529337753	0.0
2	0.0	0.0	0.0	0.711068508881885	0.0
3	0.0	0.0	0.0	2.4217510875857555	0.0
4	0.0	0.0	0.0	2.8116033202545885	0.0
5	3.922054654616023E-5	0.0	0.0	3.546125715971077	0.0
6	3.922054654616023E-5	0.0	0.0	5.729690424382002	0.0
7	3.922054654616023E-5	0.0	0.0	6.049141776000477	0.0
8	3.922054654616023E-5	0.0	0.0	6.58638482258978	0.0
9	7.844109309232046E-5	0.0	0.0	6.943448678346022	0.0
10	7.844109309232046E-5	0.0	0.0	8.270240547456076	0.0
11	7.844109309232046E-5	0.0	0.0	9.333038917763927	0.0
12	7.844109309232046E-5	0.0	0.0	9.773799419849675	0.0
13	1.1766163963848069E-4	0.0	0.0	9.997984063907527	0.0
14	1.1766163963848069E-4	0.0	0.0	10.148669403737875	0.0
15	1.1766163963848069E-4	0.0	0.0	10.351557291021162	0.0
16	1.1766163963848069E-4	0.0	0.0	10.782865641389286	0.0
17	1.1766163963848069E-4	0.0	0.0	11.307675774723457	0.0
18	1.9610273273080114E-4	0.0	0.0	11.899396160465376	0.0
19	1.9610273273080114E-4	0.0	0.0	12.314977071668489	0.0
20	1.9610273273080114E-4	0.0	0.0	12.792644108054175	0.0
21	1.9610273273080114E-4	0.0	0.0	13.491318924227473	0.0
22	1.9610273273080114E-4	0.0	0.0	14.100296350449703	0.0
23	1.9610273273080114E-4	0.0	0.0	14.692330500563992	0.0
24	2.745438258231216E-4	0.0	0.0	15.158035270253098	0.0
25	2.745438258231216E-4	0.0	0.0	15.579028616879581	0.0
26	3.1376437236928185E-4	0.0	0.0	15.965233338719623	0.0
27	3.1376437236928185E-4	0.0	0.0	16.38822693321996	0.0
28	3.1376437236928185E-4	0.0	0.0	16.762626270549607	0.0
29	3.1376437236928185E-4	0.0	0.0	17.203543654821537	0.0
30	3.1376437236928185E-4	0.0	0.0	17.651991384030335	0.0
31	3.1376437236928185E-4	0.0	0.0	18.036980268927444	0.0
32	3.1376437236928185E-4	0.0	0.0	18.463699815349667	0.0
33	3.1376437236928185E-4	0.0	0.0	18.9076764022522	0.0
34	3.1376437236928185E-4	0.0	0.0	19.393344430133304	0.0
35	3.1376437236928185E-4	0.0	0.0	19.895367425924153	0.0
36	3.1376437236928185E-4	0.0	0.0	20.297613351301575	0.0
37	3.1376437236928185E-4	0.0	0.0	20.74872807767551	0.0
38	3.1376437236928185E-4	0.0	0.0	21.27495015068534	0.0
39	3.1376437236928185E-4	0.0	0.0	22.272838516459295	0.0
40	3.1376437236928185E-4	0.0	0.0	22.757094604664736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	35	1.0202348E-7	46.000004	11
GATCGAC	50	1.6370905E-11	46.0	9
CGCATAT	20	6.312909E-4	46.0	43
TTAACGC	30	1.8623032E-6	46.0	22
CGTAAAC	40	5.6152203E-9	46.0	35
ATAACGG	30	1.8623032E-6	46.0	2
ACCGTAG	25	3.418335E-5	46.0	29
CGGTAAC	25	3.418335E-5	46.0	46
ACGGGTA	95	0.0	43.57895	5
ATCCGAT	60	1.8189894E-12	42.166668	34
AGTACGA	55	4.7293724E-11	41.81818	36
CGTTACA	45	1.5897967E-8	40.888885	31
GTATACG	45	1.5897967E-8	40.888885	1
TAAATCG	45	1.5897967E-8	40.888885	38
ACGCAGT	40	2.9124385E-7	40.25	43
GAACGTA	40	2.9124385E-7	40.25	1
CGTTATG	75	0.0	39.866665	1
TACGGGA	390	0.0	39.512817	4
ATTCGAC	35	5.3756885E-6	39.428574	11
TTATCGA	35	5.3756885E-6	39.428574	14
>>END_MODULE