FastQCFastQC Report
Thu 26 May 2016
SRR1545570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545570_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1293441
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC79630.6156446254603032TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50900.3935239411770618No Hit
ATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG36660.2834300134292944TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC30480.2356504857971875TruSeq Adapter, Index 15 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT30120.23286721234289004No Hit
ATTGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC25030.19351481822518382No Hit
ATATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC24420.18879871598317977No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT23360.1806035219233038TruSeq Adapter, Index 21 (95% over 23bp)
ATGTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC23290.18006232986274598No Hit
ATGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC21280.16452238640958497No Hit
ATGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC20250.1565591318042338No Hit
ATTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC19350.1496009481684901No Hit
ATAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC16600.1283398315037176No Hit
ATCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT16000.12370104241322179No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT253.8910774E-545.00000437
GCATAAC302.1657142E-645.00000425
TCGCCAA253.8910774E-545.00000439
ACAACGA253.8910774E-545.00000415
AAGTATT1000.045.00000417
CAGGTCG253.8910774E-545.00000420
GTGCAAC302.1657142E-645.00000412
CTAGACA502.1827873E-1145.00000439
GGTACTC253.8910774E-545.00000417
CCTTACG253.8910774E-545.0000043
TCGTTAC600.045.0000042
CGAACGA253.8910774E-545.00000426
ACACGAG253.8910774E-545.00000440
TCCGCTA302.1657142E-645.0000042
CTCGTTT253.8910774E-545.00000435
GTCGAAC253.8910774E-545.00000424
AGTCAAT453.8562575E-1045.00000432
CGTGCAT253.8910774E-545.00000423
TAGACGG600.045.0000044
CAGCCGA253.8910774E-545.00000428