##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545570_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1293441
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95940595666907	31.0	31.0	34.0	31.0	34.0
2	32.65879077592252	34.0	31.0	34.0	31.0	34.0
3	32.591096926724916	34.0	31.0	34.0	31.0	34.0
4	36.034230397830285	37.0	35.0	37.0	35.0	37.0
5	35.793061299278435	37.0	35.0	37.0	35.0	37.0
6	35.78639613248691	37.0	35.0	37.0	35.0	37.0
7	35.779989964752936	37.0	35.0	37.0	35.0	37.0
8	35.751344668987606	37.0	35.0	37.0	33.0	37.0
9	37.98477162854742	39.0	38.0	39.0	35.0	39.0
10	37.95992318165266	39.0	39.0	39.0	35.0	39.0
11	37.77064203160407	39.0	38.0	39.0	35.0	39.0
12	37.27364062218532	39.0	37.0	39.0	34.0	39.0
13	36.92762174695251	39.0	37.0	39.0	33.0	39.0
14	37.11956246941298	39.0	35.0	41.0	33.0	41.0
15	36.8499320803964	39.0	35.0	41.0	32.0	41.0
16	36.838431749109546	38.0	35.0	41.0	32.0	41.0
17	36.90357349117586	38.0	35.0	41.0	32.0	41.0
18	36.96257734214394	38.0	35.0	41.0	32.0	41.0
19	36.794902125415845	38.0	35.0	41.0	31.0	41.0
20	36.70089783762847	38.0	35.0	41.0	31.0	41.0
21	36.77162236236519	38.0	35.0	41.0	31.0	41.0
22	36.81341398641299	38.0	35.0	41.0	32.0	41.0
23	36.7780965656725	38.0	35.0	41.0	32.0	41.0
24	36.763073847202925	38.0	35.0	41.0	32.0	41.0
25	36.77452701746736	38.0	35.0	41.0	32.0	41.0
26	36.72831926620542	38.0	35.0	41.0	32.0	41.0
27	36.70139109553509	38.0	35.0	41.0	32.0	41.0
28	36.61025357940563	38.0	35.0	41.0	31.0	41.0
29	36.55463991013119	38.0	35.0	41.0	31.0	41.0
30	36.491133341219275	38.0	35.0	41.0	31.0	41.0
31	36.41423690759764	38.0	35.0	41.0	31.0	41.0
32	36.254177036293115	38.0	35.0	40.0	31.0	41.0
33	36.127724418817714	38.0	35.0	40.0	31.0	41.0
34	36.01856443394016	37.0	35.0	40.0	30.0	41.0
35	35.92350946042379	37.0	35.0	40.0	30.0	41.0
36	35.69620106367434	37.0	34.0	40.0	30.0	41.0
37	35.59236950119874	37.0	34.0	40.0	29.0	41.0
38	35.6063562234381	37.0	34.0	40.0	29.0	41.0
39	35.708488442843546	37.0	34.0	40.0	30.0	41.0
40	35.66882524985678	37.0	34.0	40.0	30.0	41.0
41	35.62380193607594	37.0	34.0	40.0	30.0	41.0
42	35.532948159212516	36.0	34.0	40.0	29.0	41.0
43	35.455648150940014	36.0	34.0	40.0	29.0	41.0
44	35.447761436354654	36.0	34.0	40.0	30.0	41.0
45	35.397372589859145	36.0	34.0	40.0	30.0	41.0
46	35.304706592724365	36.0	34.0	40.0	29.0	41.0
47	35.20149740111841	36.0	34.0	40.0	29.0	41.0
48	35.13737232699443	35.0	34.0	40.0	29.0	41.0
49	35.14818921002195	35.0	34.0	40.0	29.0	41.0
50	35.07401110680735	35.0	34.0	40.0	29.0	41.0
51	34.9426560623948	35.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	11.0
11	5.0
12	11.0
13	14.0
14	8.0
15	35.0
16	54.0
17	129.0
18	336.0
19	626.0
20	1156.0
21	1943.0
22	3113.0
23	4633.0
24	6513.0
25	9498.0
26	12826.0
27	15854.0
28	18963.0
29	22929.0
30	28093.0
31	34783.0
32	43903.0
33	58574.0
34	108837.0
35	224716.0
36	85131.0
37	111016.0
38	161922.0
39	337629.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.0806059186310006	92.62718593271745	2.943852869980154	3.348355278671389
2	3.073043146150462	2.154099027323241	92.30154293856465	2.4713148879616464
3	25.592895230628997	15.541489716191151	45.73258463277413	13.133030420405726
4	25.440974887915257	14.251287843821247	47.85428944961541	12.453447818648087
5	23.162633626118236	12.982579027570642	49.32246619675733	14.532321149553788
6	21.54261384941408	14.062179875232037	49.77103710180828	14.624169173545603
7	21.172979672053074	16.02021275033032	48.4539302527135	14.352877324903107
8	23.493224661967574	17.810476086655672	46.36175905974837	12.334540191628378
9	82.37051400102517	3.083712361058603	10.03331423698491	4.512459400931315
10	84.49685760695694	3.202078796017754	8.764991986491847	3.5360716105334533
11	79.06212962168355	5.34705487146302	9.453697540127457	6.137117966725966
12	52.23825439273999	18.577113297011614	13.876628311612205	15.308003998636197
13	44.0445292827427	19.706735753698855	20.52973425150432	15.719000712054127
14	38.48331698160179	21.576399696623195	22.29000008504447	17.65028323673055
15	26.12535090506641	28.31130295081105	27.227604506119725	18.335741638002816
16	19.226775709135556	31.891133805098182	30.103421802772605	18.778668682993658
17	19.41665680923985	21.560395874260983	41.18927728439102	17.833670032108152
18	26.13957652494393	19.779255489813604	36.766346512906274	17.314821472336195
19	23.97697305095478	18.968085904188904	29.772676140620252	27.282264904236065
20	24.96356617735173	20.98062455110051	34.069741101449544	19.98606817009821
21	28.016275964655517	25.708633018436867	27.121066983341336	19.154024033566277
22	30.75045556774526	24.91470426559851	24.162911180332152	20.171928986324076
23	27.540258890819143	28.895017244698444	25.806202215640294	17.75852164884212
24	29.68863674493077	21.881400079323292	26.348631286622275	22.08133188912366
25	26.983372260505117	24.26055769068709	24.71515902155568	24.04091102725211
26	30.23547266554872	18.17516222232015	31.190367399827284	20.398997712303846
27	30.47181896970948	20.44878738187517	26.136638625186613	22.942755023228738
28	21.390693506700345	24.561769728963284	26.794805483976464	27.25273128035991
29	18.86719224147062	25.025803264315883	28.98323155056937	27.123772943644127
30	19.21981752549981	27.33151338174683	30.67724001326694	22.771429079486424
31	22.422282887275106	24.485384335273118	29.413943117621912	23.678389659829865
32	23.993672691680565	28.20561587269926	29.23635480860743	18.564356627012753
33	30.464087654558654	26.495371648184957	24.33671114492273	18.70382955233366
34	33.05778926135788	23.56651752959741	25.707859886921785	17.667833322122924
35	32.029524346297976	26.604151252357084	22.91322139935258	18.45310300199236
36	24.827495030697186	22.30275675504333	30.015284810053185	22.8544634042063
37	21.46120310087588	27.204642500121768	31.514773383555955	19.819381015446393
38	23.642284418075505	32.3936692899019	24.52148957702748	19.442556714995117
39	24.984208788804438	31.5123766758592	24.379078751949258	19.124335783387107
40	25.283410685141416	29.590371729363767	23.935687828049364	21.19052975744545
41	25.723013264617407	29.29843726926856	22.08558411245662	22.892965353657416
42	27.775677437161804	23.163097505027288	22.310488070194157	26.75073698761675
43	20.92789698177188	25.48218279766916	23.8789399748423	29.710980245716655
44	19.30795451821923	25.31433594574472	23.49825001681561	31.879459519220436
45	18.756093242753245	21.72739228151883	26.017653685015397	33.49886079071253
46	22.078625928820873	21.33425490609931	26.61110943599283	29.976009729086982
47	21.56186482413964	24.66444159416626	26.670253996896648	27.10343958479745
48	22.622755889136034	27.36669086568309	24.36871878964715	25.64183445553373
49	21.708991751459866	24.02281974979918	27.378287838409328	26.889900660331627
50	20.696807971913678	20.869757491837664	34.17913921083374	24.254295325414923
51	23.577418683960072	19.208065926470557	29.82888280176676	27.38563258780261
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	723.0
1	1044.0
2	1365.0
3	4944.5
4	8524.0
5	5437.0
6	2350.0
7	2425.0
8	2500.0
9	2591.5
10	2683.0
11	2767.5
12	2852.0
13	2692.0
14	2532.0
15	2402.0
16	2272.0
17	2450.0
18	2628.0
19	2340.0
20	2052.0
21	2088.0
22	2124.0
23	3095.0
24	4066.0
25	4323.0
26	6285.0
27	7990.0
28	8874.5
29	9759.0
30	13272.5
31	16786.0
32	18113.5
33	19441.0
34	22795.5
35	26150.0
36	30499.0
37	34848.0
38	46102.5
39	57357.0
40	69919.0
41	82481.0
42	100416.5
43	118352.0
44	127359.0
45	136366.0
46	124342.0
47	112318.0
48	103696.5
49	95075.0
50	87410.5
51	79746.0
52	81257.0
53	82768.0
54	81793.0
55	80818.0
56	84024.0
57	87230.0
58	82291.0
59	77352.0
60	65324.0
61	53296.0
62	45160.0
63	37024.0
64	27157.0
65	17290.0
66	13503.5
67	9717.0
68	7022.0
69	4327.0
70	3219.5
71	2112.0
72	2018.0
73	1924.0
74	1304.0
75	639.0
76	594.0
77	456.5
78	319.0
79	185.5
80	52.0
81	30.0
82	8.0
83	4.5
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1293441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.507014099504415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.86203761592806	10.678445882307999
2	12.243368671135277	3.797161812174507
3	5.572901605637584	2.5925719132131846
4	3.117973397039013	1.934018297190546
5	1.860129649029171	1.4422528347200776
6	1.2686671585456568	1.1803943709087423
7	0.8688397494130745	0.9431177171050902
8	0.62039257011662	0.7696349065620965
9	0.446949096409541	0.623776134580516
>10	2.6177785182594726	8.052242977070371
>50	0.7163471149808721	8.298900865129827
>100	1.781119867878349	54.68839004947961
>500	0.01499679933661254	1.5075802985767006
>1k	0.00749839966830627	2.4796626177947094
>5k	9.99786622440836E-4	1.0118493231860177
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	7963	0.6156446254603032	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5090	0.3935239411770618	No Hit
ATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	3666	0.2834300134292944	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3048	0.2356504857971875	TruSeq Adapter, Index 15 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	3012	0.23286721234289004	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	2503	0.19351481822518382	No Hit
ATATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	2442	0.18879871598317977	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2336	0.1806035219233038	TruSeq Adapter, Index 21 (95% over 23bp)
ATGTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	2329	0.18006232986274598	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	2128	0.16452238640958497	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	2025	0.1565591318042338	No Hit
ATTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	1935	0.1496009481684901	No Hit
ATAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	1660	0.1283398315037176	No Hit
ATCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1600	0.12370104241322179	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1927416867101012	0.0
2	0.0	0.0	0.0	1.1178708576579837	0.0
3	0.0	0.0	0.0	1.5001843918663471	0.0
4	0.0	0.0	0.0	2.019883396304895	0.0
5	0.0	0.0	0.0	3.808291216994049	0.0
6	0.0	0.0	0.0	4.1934653378082185	0.0
7	0.0	0.0	0.0	4.870341979263067	0.0
8	0.0	0.0	0.0	6.937618337442528	0.0
9	0.0	0.0	0.0	7.194452626752979	0.0
10	0.0	0.0	0.0	7.70379166888942	0.0
11	0.0	0.0	0.0	8.106825127701997	0.0
12	0.0	0.0	0.0	9.199337271665271	0.0
13	7.731315150826361E-5	0.0	0.0	10.166834049639682	0.0
14	7.731315150826361E-5	0.0	0.0	10.564842153604223	0.0
15	1.5462630301652723E-4	0.0	0.0	10.747146564860708	0.0
16	1.5462630301652723E-4	0.0	0.0	10.856312734790377	0.0
17	1.5462630301652723E-4	0.0	0.0	10.999496691383682	0.0
18	1.5462630301652723E-4	0.0	0.0	11.384438872743326	0.0
19	1.5462630301652723E-4	0.0	0.0	11.851642247307764	0.0
20	2.3193945452479084E-4	0.0	0.0	12.388659397684162	0.0
21	3.0925260603305446E-4	0.0	0.0	12.758138948742154	0.0
22	3.0925260603305446E-4	0.0	0.0	13.183670534643637	0.0
23	3.865657575413181E-4	0.0	0.0	13.80333544398237	0.0
24	3.865657575413181E-4	0.0	0.0	14.320869680178687	0.0
25	4.638789090495817E-4	0.0	0.0	14.858350709464135	0.0
26	6.185052120661089E-4	0.0	0.0	15.308931756454296	0.0
27	6.958183635743725E-4	0.0	0.0	15.702146445025324	0.0
28	7.731315150826362E-4	0.0	0.0	16.05631799208468	0.0
29	7.731315150826362E-4	0.0	0.0	16.357066151451825	0.0
30	7.731315150826362E-4	0.0	0.0	16.66941128354521	0.0
31	7.731315150826362E-4	0.0	0.0	16.98013283945692	0.0
32	7.731315150826362E-4	0.0	0.0	17.35370998754485	0.0
33	7.731315150826362E-4	0.0	0.0	17.698217390665675	0.0
34	7.731315150826362E-4	0.0	0.0	18.03638511536282	0.0
35	7.731315150826362E-4	0.0	0.0	18.398056038118476	0.0
36	7.731315150826362E-4	0.0	0.0	18.796759960446593	0.0
37	8.504446665908998E-4	0.0	0.0	19.23311538755923	0.0
38	8.504446665908998E-4	0.0	0.0	19.617207124252285	0.0
39	8.504446665908998E-4	0.0	0.0	20.018771633186205	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	25	3.8910774E-5	45.000004	37
GCATAAC	30	2.1657142E-6	45.000004	25
TCGCCAA	25	3.8910774E-5	45.000004	39
ACAACGA	25	3.8910774E-5	45.000004	15
AAGTATT	100	0.0	45.000004	17
CAGGTCG	25	3.8910774E-5	45.000004	20
GTGCAAC	30	2.1657142E-6	45.000004	12
CTAGACA	50	2.1827873E-11	45.000004	39
GGTACTC	25	3.8910774E-5	45.000004	17
CCTTACG	25	3.8910774E-5	45.000004	3
TCGTTAC	60	0.0	45.000004	2
CGAACGA	25	3.8910774E-5	45.000004	26
ACACGAG	25	3.8910774E-5	45.000004	40
TCCGCTA	30	2.1657142E-6	45.000004	2
CTCGTTT	25	3.8910774E-5	45.000004	35
GTCGAAC	25	3.8910774E-5	45.000004	24
AGTCAAT	45	3.8562575E-10	45.000004	32
CGTGCAT	25	3.8910774E-5	45.000004	23
TAGACGG	60	0.0	45.000004	4
CAGCCGA	25	3.8910774E-5	45.000004	28
>>END_MODULE