FastQCFastQC Report
Thu 26 May 2016
SRR1545569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545569_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1094327
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT60530.5531253455320028TruSeq Adapter, Index 21 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC46650.4262893997863527TruSeq Adapter, Index 21 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC44980.4110288789365518TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT43900.4011597995845849TruSeq Adapter, Index 15 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC42480.3881837878440356TruSeq Adapter, Index 21 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC33690.3078604475627486TruSeq Adapter, Index 21 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC31130.28446707428401197TruSeq Adapter, Index 15 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC31120.2843756939196419TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT21900.2001229979704421TruSeq Adapter, Index 15 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC20880.19080220080469548TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC18800.171795085015722TruSeq Adapter, Index 21 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC14880.13597398218265655TruSeq Adapter, Index 21 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC12810.11705824675805312TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT11140.10179772590825228TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG206.31132E-446.0000046
ATCCTTA206.31132E-446.00000410
CTTGTAC351.0195072E-746.00000416
TTGATAC351.0195072E-746.00000430
ACAACCC206.31132E-446.00000428
AGCGTTA206.31132E-446.00000420
GTGCAAC351.0195072E-746.00000410
AACGTAC351.0195072E-746.00000418
AAGTACG351.0195072E-746.00000425
AGCGTAC206.31132E-446.00000431
CGTGGAC206.31132E-446.00000414
CGGAAAG351.0195072E-746.00000420
GGTACTC405.6115823E-946.00000415
ACAGTTC405.6115823E-946.00000428
GCCCATA206.31132E-446.00000437
CCGGGTT206.31132E-446.00000435
GCCTTCG206.31132E-446.0000041
AGTCCCG206.31132E-446.00000411
AGTCCCC206.31132E-446.00000420
TCGCAGA206.31132E-446.00000414
Produced by FastQC (version 0.11.5)