##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545569_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1094327
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61645467945139	33.0	31.0	34.0	31.0	34.0
2	33.00472345103429	34.0	33.0	34.0	31.0	34.0
3	33.04826893606756	34.0	33.0	34.0	31.0	34.0
4	36.414186984329184	37.0	37.0	37.0	35.0	37.0
5	36.39403487257465	37.0	37.0	37.0	35.0	37.0
6	36.401029125663534	37.0	37.0	37.0	35.0	37.0
7	36.509633774913716	37.0	37.0	37.0	35.0	37.0
8	36.568444349815	37.0	37.0	37.0	35.0	37.0
9	38.382175528886705	39.0	39.0	39.0	37.0	39.0
10	38.138951154453835	39.0	39.0	39.0	35.0	39.0
11	37.8983978280715	39.0	38.0	39.0	35.0	39.0
12	37.16713011741463	39.0	35.0	39.0	35.0	39.0
13	37.01970434796912	39.0	35.0	39.0	35.0	39.0
14	38.132881670652374	40.0	35.0	41.0	35.0	41.0
15	38.11379322633911	40.0	35.0	41.0	35.0	41.0
16	38.1800019555398	40.0	35.0	41.0	35.0	41.0
17	37.99727686514177	40.0	35.0	41.0	34.0	41.0
18	37.92200868661744	40.0	35.0	41.0	34.0	41.0
19	37.96169426505971	40.0	35.0	41.0	34.0	41.0
20	38.01211612251183	40.0	35.0	41.0	34.0	41.0
21	37.986408084603596	40.0	35.0	41.0	34.0	41.0
22	38.120061919334894	40.0	35.0	41.0	34.0	41.0
23	38.17291449447926	40.0	35.0	41.0	35.0	41.0
24	38.163197106532145	40.0	35.0	41.0	35.0	41.0
25	38.1420599144497	40.0	35.0	41.0	35.0	41.0
26	38.03063252574413	40.0	35.0	41.0	35.0	41.0
27	37.94767834477263	40.0	35.0	41.0	34.0	41.0
28	37.92225358599395	40.0	35.0	41.0	34.0	41.0
29	37.91163884286872	40.0	35.0	41.0	34.0	41.0
30	37.81347348644418	40.0	35.0	41.0	34.0	41.0
31	37.730066058865404	40.0	35.0	41.0	34.0	41.0
32	37.6488746051226	40.0	35.0	41.0	34.0	41.0
33	37.576457493966615	40.0	35.0	41.0	34.0	41.0
34	37.3501174694584	39.0	35.0	41.0	33.0	41.0
35	37.297772969139935	39.0	35.0	41.0	33.0	41.0
36	37.31542765553623	39.0	35.0	41.0	33.0	41.0
37	37.375683867801854	39.0	35.0	41.0	34.0	41.0
38	37.332876736112695	39.0	35.0	41.0	34.0	41.0
39	37.27505032773568	39.0	35.0	41.0	34.0	41.0
40	37.1841067615073	39.0	35.0	41.0	34.0	41.0
41	37.087431818825635	38.0	35.0	41.0	34.0	41.0
42	37.109336605968785	38.0	35.0	41.0	33.0	41.0
43	37.03698163346056	38.0	35.0	41.0	33.0	41.0
44	36.90285079322725	38.0	35.0	41.0	33.0	41.0
45	36.835339893834295	38.0	35.0	41.0	33.0	41.0
46	36.831676455026695	38.0	35.0	41.0	33.0	41.0
47	36.84616572560122	38.0	35.0	41.0	33.0	41.0
48	36.77916747005237	38.0	35.0	41.0	33.0	41.0
49	36.769647463692294	38.0	35.0	41.0	33.0	41.0
50	36.691295197870474	37.0	35.0	40.0	33.0	41.0
51	36.60636171820671	37.0	35.0	40.0	33.0	41.0
52	36.33818502147896	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	11.0
13	23.0
14	33.0
15	43.0
16	66.0
17	104.0
18	112.0
19	247.0
20	452.0
21	687.0
22	1008.0
23	1527.0
24	2184.0
25	3037.0
26	3915.0
27	4982.0
28	6397.0
29	8200.0
30	10712.0
31	14169.0
32	19452.0
33	28118.0
34	86547.0
35	194432.0
36	58878.0
37	72604.0
38	127138.0
39	448444.0
40	799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.39542568172036	13.782717597208146	48.463393482935174	12.358463238136316
2	23.444729043512588	12.171864534092643	53.02345642573015	11.359949996664618
3	23.88454273722571	12.715760462823269	47.611362965548686	15.788333834402332
4	22.158550415003926	13.961457589915993	49.6822247829031	14.19776721217698
5	21.92498220367404	15.735790124889542	47.40082260604006	14.938405065396356
6	22.569213772482996	17.82693838313411	46.74992027063209	12.853927573750806
7	84.42503931640178	2.2948350904254395	7.832302410522632	5.447823182650159
8	87.29694140782416	2.02791304610048	7.08362308523869	3.5915224608366603
9	80.40430328411891	3.400720260031965	9.08348235947756	7.111494096371559
10	52.51401089436703	17.905982398314215	12.301807412226875	17.27819929509187
11	43.633118802697915	18.270772812879514	21.734271383233715	16.36183700118886
12	38.622276522465405	21.738657640723478	21.502256638098117	18.136809198713
13	26.495919409829057	26.21291442137497	28.60050058163602	18.690665587159963
14	18.92597002541288	32.40265478234568	29.971114666822622	18.70026052541882
15	19.018446954155387	21.343346184458575	40.08079851817602	19.557408343210028
16	26.099328628462974	21.07880002960724	34.74400247823548	18.077868863694306
17	24.00955107568396	20.145258227202657	28.14405566160755	27.701135035505843
18	24.626825437003745	21.543743323522126	32.25132889894885	21.578102340525273
19	26.20697469769091	27.26470241527441	27.05635518451066	19.47196770252402
20	30.39566783968594	24.330661676080368	22.760747016202654	22.512923468031037
21	26.986906107589416	30.410471458713893	25.383820375445364	17.218802058251327
22	28.587707330624212	22.19610774476002	26.143465344453716	23.072719580162055
23	26.80953682034712	25.39030838131564	22.920297132392786	24.879857665944456
24	30.472153204663687	17.40741113031114	29.0642559308141	23.05617973421107
25	32.37478377121281	19.60465199158935	23.66915921840547	24.35140501879237
26	21.1323489231281	25.003952200759006	25.347816511883558	28.515882364229338
27	18.695051844649726	22.713960269645177	29.187802183442425	29.403185702262668
28	18.666175649508784	27.858217881857982	29.802792035652963	23.67281443298027
29	21.68848982068431	23.836202524473947	29.355485152061494	25.11982250278025
30	24.118019568191226	28.20582878792171	29.043329827373356	18.63282181651371
31	30.80989503137545	27.458520168103316	22.876069036037673	18.85551576448356
32	33.895261653966315	23.599984282577328	24.36127409814434	18.143479965312014
33	31.64109082568556	28.343630377391765	21.508470502875284	18.506808294047392
34	24.598588904413397	21.723854021695526	29.313907086273115	24.36364998761796
35	21.598571542144168	27.383862410412974	30.87212505951146	20.145440987931394
36	22.423462091312743	32.27453951149885	24.40550219449945	20.896496202688958
37	25.918395507010246	30.727469942713647	22.975856393929785	20.378278156346322
38	24.214243091872905	28.988044706929468	25.381901387793594	21.415810813404036
39	26.767227711643777	29.034009030207606	20.903166969287973	23.295596288860644
40	28.861482902276926	22.720265514786714	22.723189686446556	25.695061896489808
41	21.147152542156046	24.878578340843276	23.830262800789892	30.144006316210785
42	19.483390248070275	25.248486055813295	22.50396819232277	32.76415550379366
43	18.859262359422733	20.682392008969895	26.750413724599685	33.70793190700769
44	24.74233021756751	20.135206387121947	24.466087376076803	30.65637601923374
45	21.53195525651839	22.836135816807957	26.897079209413636	28.734829717260013
46	22.78450591093887	26.23667331611118	24.013297670623132	26.965523102326816
47	22.201773327350967	23.359471163555316	27.63917914846294	26.799576360630777
48	19.71768950231512	19.874589587938523	35.74982614885678	24.65789476088957
49	23.386062849587006	18.131874659037013	28.83818090936256	29.64388158201342
50	20.569994160794717	21.44505253000246	31.38531718581375	26.599636123389082
51	21.226836219886742	22.436803624510773	29.338214263195557	26.99814589240693
52	18.67503954485268	19.718694686323193	30.09530058200154	31.510965186822588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	263.0
2	186.0
3	154.0
4	122.0
5	145.0
6	168.0
7	243.0
8	318.0
9	427.5
10	537.0
11	512.5
12	488.0
13	614.5
14	635.0
15	529.0
16	626.5
17	724.0
18	699.5
19	675.0
20	604.0
21	533.0
22	968.0
23	1403.0
24	1708.0
25	2013.0
26	2612.0
27	3211.0
28	4176.0
29	5141.0
30	6380.5
31	7620.0
32	9873.5
33	12127.0
34	13544.0
35	14961.0
36	14723.5
37	14486.0
38	20344.5
39	31629.5
40	37056.0
41	52441.5
42	67827.0
43	78817.0
44	89807.0
45	103765.5
46	117724.0
47	119199.5
48	120675.0
49	108579.0
50	96483.0
51	83234.0
52	69985.0
53	70681.0
54	71377.0
55	68901.5
56	66426.0
57	64305.0
58	62184.0
59	61408.0
60	60632.0
61	57690.0
62	54748.0
63	46174.0
64	29849.5
65	22099.0
66	17161.5
67	12224.0
68	9478.0
69	6732.0
70	4986.0
71	3240.0
72	2667.0
73	2094.0
74	1656.0
75	1218.0
76	927.0
77	636.0
78	585.0
79	534.0
80	376.0
81	218.0
82	203.0
83	188.0
84	112.5
85	37.0
86	28.5
87	20.0
88	16.0
89	12.0
90	12.0
91	9.5
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1094327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.698729275182782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.13034829402334	9.910662469158696
2	13.615621234488215	4.27495903347321
3	6.302458996942844	2.968217926828372
4	3.8087940601312744	2.391729072597005
5	2.377074814940315	1.865852699330161
6	1.6226403058133931	1.5284034523178553
7	1.191019542641741	1.3088245352969292
8	0.8594308357217324	1.0793577616591652
9	0.6627488811560439	0.936388373642912
>10	3.694738462760262	11.286313646862297
>50	1.0130738295274322	11.966938540009037
>100	1.7034087852433457	45.43021212438562
>500	0.01048610059315329	1.0748670523016646
>1k	0.007573294872832932	3.4236980319888635
>5k	5.825611440640718E-4	0.5535752801482153
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT	6053	0.5531253455320028	TruSeq Adapter, Index 21 (95% over 24bp)
TTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	4665	0.4262893997863527	TruSeq Adapter, Index 21 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	4498	0.4110288789365518	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	4390	0.4011597995845849	TruSeq Adapter, Index 15 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	4248	0.3881837878440356	TruSeq Adapter, Index 21 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3369	0.3078604475627486	TruSeq Adapter, Index 21 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3113	0.28446707428401197	TruSeq Adapter, Index 15 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3112	0.2843756939196419	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2190	0.2001229979704421	TruSeq Adapter, Index 15 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2088	0.19080220080469548	TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1880	0.171795085015722	TruSeq Adapter, Index 21 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1488	0.13597398218265655	TruSeq Adapter, Index 21 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1281	0.11705824675805312	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	1114	0.10179772590825228	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5679289645599532	0.0
2	0.0	0.0	0.0	1.3218169706129885	0.0
3	0.0	0.0	0.0	4.468317056967433	0.0
4	0.0	0.0	0.0	5.095826019096669	0.0
5	0.0	0.0	0.0	6.203356035261855	0.0
6	0.0	0.0	0.0	9.610929822621575	0.0
7	0.0	0.0	0.0	10.049829712690997	0.0
8	0.0	0.0	0.0	10.92753811246547	0.0
9	0.0	0.0	0.0	11.529917474392937	0.0
10	0.0	0.0	0.0	13.411987458958794	0.0
11	0.0	0.0	0.0	15.001274756082962	0.0
12	0.0	0.0	0.0	15.65994442246239	0.0
13	0.0	0.0	0.0	15.962047907069824	0.0
14	0.0	0.0	0.0	16.1074340667826	0.0
15	0.0	0.0	0.0	16.33716430280894	0.0
16	0.0	0.0	0.0	16.921450352591137	0.0
17	0.0	0.0	0.0	17.667571027672714	0.0
18	0.0	0.0	0.0	18.542994918337936	0.0
19	1.8276072874012978E-4	0.0	0.0	19.133677593626036	0.0
20	1.8276072874012978E-4	0.0	0.0	19.792712781462946	0.0
21	1.8276072874012978E-4	0.0	0.0	20.751201423340554	0.0
22	2.741410931101947E-4	0.0	0.0	21.550048568663662	0.0
23	3.6552145748025957E-4	0.0	0.0	22.331076542934607	0.0
24	3.6552145748025957E-4	0.0	0.0	23.01323096295714	0.0
25	3.6552145748025957E-4	0.0	0.0	23.61359995686847	0.0
26	3.6552145748025957E-4	0.0	0.0	24.150002695720747	0.0
27	4.5690182185032446E-4	0.0	0.0	24.58908534651891	0.0
28	4.5690182185032446E-4	0.0	0.0	25.033011156628685	0.0
29	4.5690182185032446E-4	0.0	0.0	25.47510935945106	0.0
30	4.5690182185032446E-4	0.0	0.0	25.978889308223227	0.0
31	4.5690182185032446E-4	0.0	0.0	26.441365332300126	0.0
32	4.5690182185032446E-4	0.0	0.0	26.905120681478206	0.0
33	4.5690182185032446E-4	0.0	0.0	27.395467716687975	0.0
34	4.5690182185032446E-4	0.0	0.0	27.937170516673717	0.0
35	4.5690182185032446E-4	0.0	0.0	28.534615338925203	0.0
36	4.5690182185032446E-4	0.0	0.0	29.03784700551115	0.0
37	4.5690182185032446E-4	0.0	0.0	29.546561493959302	0.0
38	4.5690182185032446E-4	0.0	0.0	30.158170272688146	0.0
39	4.5690182185032446E-4	0.0	0.0	30.793903467610686	0.0
40	4.5690182185032446E-4	0.0	0.0	31.565427883987144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	6.31132E-4	46.000004	6
ATCCTTA	20	6.31132E-4	46.000004	10
CTTGTAC	35	1.0195072E-7	46.000004	16
TTGATAC	35	1.0195072E-7	46.000004	30
ACAACCC	20	6.31132E-4	46.000004	28
AGCGTTA	20	6.31132E-4	46.000004	20
GTGCAAC	35	1.0195072E-7	46.000004	10
AACGTAC	35	1.0195072E-7	46.000004	18
AAGTACG	35	1.0195072E-7	46.000004	25
AGCGTAC	20	6.31132E-4	46.000004	31
CGTGGAC	20	6.31132E-4	46.000004	14
CGGAAAG	35	1.0195072E-7	46.000004	20
GGTACTC	40	5.6115823E-9	46.000004	15
ACAGTTC	40	5.6115823E-9	46.000004	28
GCCCATA	20	6.31132E-4	46.000004	37
CCGGGTT	20	6.31132E-4	46.000004	35
GCCTTCG	20	6.31132E-4	46.000004	1
AGTCCCG	20	6.31132E-4	46.000004	11
AGTCCCC	20	6.31132E-4	46.000004	20
TCGCAGA	20	6.31132E-4	46.000004	14
>>END_MODULE