FastQCFastQC Report
Thu 26 May 2016
SRR1545568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545568_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1056000
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC94090.8910037878787879No Hit
ATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG45430.4302083333333333No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.39214015151515147No Hit
ATGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT31490.2982007575757576No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC30050.2845643939393939No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC27330.25880681818181817No Hit
ATATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC25510.2415719696969697No Hit
ATGCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC25220.23882575757575758No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT24490.2319128787878788No Hit
ATGTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC24450.23153409090909094No Hit
ATTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC21580.2043560606060606No Hit
ATGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC19550.18513257575757575No Hit
ATCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT16710.15823863636363636No Hit
ATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC14740.13958333333333334No Hit
ATAGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC14330.13570075757575759No Hit
ATTCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC13540.12821969696969696No Hit
ATCTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC12150.11505681818181819No Hit
ATGGATAGGAGGTGGAGAAGGATCCTTTAGAAGAAGAAGTTATATGATATA12120.11477272727272728No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT11440.10833333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT253.8905888E-545.00000429
ACAACGC253.8905888E-545.00000424
AAACTCG207.0330244E-445.00000418
CTATGCG207.0330244E-445.0000043
CTGTCGA207.0330244E-445.00000413
TTGGCCG253.8905888E-545.0000043
CGAACCT502.1827873E-1145.00000443
ACGCCAT253.8905888E-545.00000438
CGTATCA207.0330244E-445.00000413
GGTCGTT207.0330244E-445.00000410
CTGTAGT207.0330244E-445.00000416
CGTATAG800.045.0000043
CGAGTCG207.0330244E-445.00000417
CTCCGAC207.0330244E-445.00000417
CGACGTG207.0330244E-445.00000433
CCGATGG253.8905888E-545.00000418
AGCCCGA207.0330244E-445.00000416
ACCGCTA207.0330244E-445.00000426
GTCCTAC207.0330244E-445.00000439
CGTTGTA406.8139343E-945.00000437
Produced by FastQC (version 0.11.5)