SRR1545567_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545567_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	899071
Sequences flagged as poor quality	0
Sequence length	52
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	7726	0.859331465479367	Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	5375	0.597839325259073	No Hit
TGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	5240	0.5828238259269847	No Hit
GTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	4677	0.5202036324161273	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	4554	0.5065228441357802	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3822	0.4251054699795678	No Hit
ATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3678	0.4090889373586736	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3291	0.3660445059400203	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	2531	0.2815128059964118	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2264	0.25181548509517043	No Hit
CTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2173	0.24169392628613312	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1875	0.20854860183456034	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1863	0.20721389078281913	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1504	0.167283785151562	No Hit
GACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1175	0.13069045714965782	No Hit
TACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1133	0.12601896846856367	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	952	0.10588707677146743	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	916	0.10188294361624388	No Hit
AACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	912	0.10143803993233015	No Hit
CCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	910	0.10121558809037329	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	35	1.0192343E-7	46.000004	11
GTCGCGT	35	1.0192343E-7	46.000004	18
TCGCAAT	35	1.0192343E-7	46.000004	30
CGTATTG	55	1.8189894E-12	46.000004	1
GCGACGT	35	1.0192343E-7	46.000004	17
GCGACGA	35	1.0192343E-7	46.000004	10
CTGTATT	35	1.0192343E-7	46.000004	33
GGTAATC	110	0.0	46.000004	8
CGAGTCG	35	1.0192343E-7	46.000004	15
CAACGGA	55	1.8189894E-12	46.000004	37
ACTGACG	55	1.8189894E-12	46.000004	1
CGTGCCC	35	1.0192343E-7	46.000004	20
ATAATCG	35	1.0192343E-7	46.000004	27
ATTACGC	35	1.0192343E-7	46.000004	13
GCTATCA	35	1.0192343E-7	46.000004	9
CTCCCGG	35	1.0192343E-7	46.000004	23
TTCACGA	35	1.0192343E-7	46.000004	26
GCTGCGA	35	1.0192343E-7	46.000004	31
CCTACCG	35	1.0192343E-7	46.000004	38
ATTCGCT	35	1.0192343E-7	46.000004	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content