##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545567_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	899071
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54864521266952	33.0	31.0	34.0	31.0	34.0
2	32.923747957614026	34.0	31.0	34.0	31.0	34.0
3	32.984634139016826	34.0	33.0	34.0	31.0	34.0
4	36.3704301440042	37.0	37.0	37.0	35.0	37.0
5	36.34752205331948	37.0	37.0	37.0	35.0	37.0
6	36.340097723094175	37.0	37.0	37.0	35.0	37.0
7	36.45160170887505	37.0	37.0	37.0	35.0	37.0
8	36.51507278068139	37.0	37.0	37.0	35.0	37.0
9	38.31436671853502	39.0	39.0	39.0	37.0	39.0
10	38.06192169472711	39.0	39.0	39.0	35.0	39.0
11	37.849066425232266	39.0	38.0	39.0	35.0	39.0
12	37.31598171890763	39.0	35.0	39.0	35.0	39.0
13	37.18303003878448	39.0	35.0	39.0	34.0	39.0
14	38.43457079585483	40.0	36.0	41.0	35.0	41.0
15	38.51466680606982	40.0	36.0	41.0	35.0	41.0
16	38.60006050690101	40.0	36.0	41.0	35.0	41.0
17	38.56688515145078	40.0	36.0	41.0	35.0	41.0
18	38.611229813885664	40.0	36.0	41.0	35.0	41.0
19	38.59261726826914	40.0	36.0	41.0	35.0	41.0
20	38.48399292158239	40.0	36.0	41.0	35.0	41.0
21	38.46103366697402	40.0	36.0	41.0	35.0	41.0
22	38.520143570418796	40.0	36.0	41.0	35.0	41.0
23	38.530844616276134	40.0	36.0	41.0	35.0	41.0
24	38.50747827479699	40.0	35.0	41.0	35.0	41.0
25	38.46227383599293	40.0	35.0	41.0	35.0	41.0
26	38.35878034104092	40.0	35.0	41.0	35.0	41.0
27	38.246335383968564	40.0	35.0	41.0	35.0	41.0
28	38.186707167732024	40.0	36.0	41.0	35.0	41.0
29	37.96947182146905	40.0	35.0	41.0	34.0	41.0
30	38.06420849966243	40.0	36.0	41.0	34.0	41.0
31	37.99386589045804	40.0	35.0	41.0	34.0	41.0
32	37.7276733428172	40.0	35.0	41.0	34.0	41.0
33	37.830882099411504	40.0	35.0	41.0	34.0	41.0
34	37.8359061742621	40.0	35.0	41.0	34.0	41.0
35	37.84132176435454	40.0	35.0	41.0	34.0	41.0
36	37.640366556145175	40.0	35.0	41.0	34.0	41.0
37	37.48396177832451	40.0	35.0	41.0	33.0	41.0
38	37.51083618535132	39.0	35.0	41.0	33.0	41.0
39	37.5520298174449	40.0	35.0	41.0	34.0	41.0
40	37.46817770787847	40.0	35.0	41.0	33.0	41.0
41	37.39622009830147	39.0	35.0	41.0	33.0	41.0
42	37.34973433688774	39.0	35.0	41.0	33.0	41.0
43	37.257927349452935	39.0	35.0	41.0	33.0	41.0
44	37.11429019510139	39.0	35.0	41.0	33.0	41.0
45	37.01564503804482	39.0	35.0	41.0	33.0	41.0
46	37.0707307876686	39.0	35.0	41.0	33.0	41.0
47	37.05514247484348	39.0	35.0	41.0	33.0	41.0
48	36.98650495900769	39.0	35.0	41.0	33.0	41.0
49	36.98201031954095	39.0	35.0	41.0	33.0	41.0
50	36.89498270993058	39.0	35.0	41.0	33.0	41.0
51	36.7883548685254	38.0	35.0	41.0	32.0	41.0
52	36.41289953741139	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	5.0
14	7.0
15	5.0
16	23.0
17	40.0
18	75.0
19	172.0
20	295.0
21	438.0
22	747.0
23	1225.0
24	1852.0
25	2617.0
26	3246.0
27	4154.0
28	5321.0
29	6958.0
30	8804.0
31	11587.0
32	15272.0
33	21303.0
34	50332.0
35	137514.0
36	46889.0
37	62565.0
38	113876.0
39	403182.0
40	560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.014296979882566	13.745744218198563	47.714474162774685	12.525484639144183
2	23.979752433345087	14.057065571017194	49.380416007189645	12.582765988448074
3	23.702466212345854	14.284633805339068	45.88247201833893	16.13042796397615
4	20.00242472507733	15.360188461200504	50.5925560940126	14.044830719709566
5	23.549975474684423	16.23542523337979	45.41343230957288	14.801166982362906
6	22.002044332427584	17.18151291722233	46.526136423041116	14.290306327308965
7	81.65172717171392	2.4396293507409315	8.941563013377142	6.967080464168013
8	84.55071957609577	2.3892440085377014	8.805200034257583	4.2548363811089445
9	75.6108249515333	4.059412437949839	11.341262258486816	8.98850035203004
10	42.36873394870928	24.004444587802297	15.235837881546619	18.390983581941807
11	35.39242173310006	19.54906787116924	29.53203918266744	15.526471213063262
12	32.48219551069938	24.483049725772492	24.19608684964814	18.838667913879995
13	19.587107136143864	32.98927448444005	33.494907521208	13.928710858208085
14	13.778889542650136	41.40240314724866	28.418222809989423	16.40048450011178
15	12.18157409147887	25.52946319033758	41.238233687884495	21.050729030299053
16	16.050122849029723	24.351691913096964	40.118522341394616	19.479662896478697
17	14.58027230329974	24.474151652094218	26.77830783108342	34.167268213522625
18	17.56045962999585	24.95131085309169	32.53246962698163	24.955759889930828
19	21.505865498942796	27.731958877552497	29.17789585027211	21.58427977323259
20	23.654082936720236	23.792336756496425	26.08025395102278	26.473326355760555
21	19.8143416927028	31.912162665684914	28.982138229350074	19.291357412262215
22	25.916306943500565	22.344508943120175	24.88602123747735	26.853162875901905
23	20.980434248240684	30.380025604207013	21.15950798101596	27.480032166536343
24	20.598706887442706	22.299351219202933	33.83592619492787	23.266015698426486
25	26.395468211075656	24.187744905574753	27.206416400929406	22.210370482420185
26	18.367403686694377	32.92843390566485	24.52576047942821	24.178401928212566
27	22.76371943928789	28.3187868366347	23.599026105835915	25.318467618241495
28	22.50656510998575	25.88794433365107	31.374274111833216	20.231216444529963
29	17.32010041476146	22.400121903609392	34.63007927071388	25.649698410915267
30	22.71956274865945	32.019050775745185	27.258136454184374	18.00325002141099
31	25.187109805565967	25.428358828168186	27.716720926378454	21.667810439887393
32	24.128127811930312	25.461726604461717	29.534708604770927	20.875436978837044
33	23.22530701134838	29.568966188432285	22.700098212488225	24.505628587731113
34	17.68981537609377	27.353123390699956	29.435606309179143	25.521454924027136
35	19.98663064429839	24.179402961501374	33.60157317942632	22.232393214773914
36	24.277837901567285	24.492059025371745	25.069432781170786	26.160670291890188
37	21.4078754625608	32.311463721997484	27.646648596162038	18.63401221927968
38	17.325105581205488	27.814266059076537	33.816572884677626	21.044055475040345
39	17.583928299322302	30.741064943703	27.902913118096347	23.772093638878353
40	24.87923645629767	22.18734671677765	29.322489547544077	23.610927279380604
41	20.321976796048364	24.40641506621835	28.531673249387424	26.739934888345857
42	20.526298812885745	26.69700168284819	24.34991229836131	28.426787205904763
43	20.693026468432414	22.291454178813463	28.85534067943466	28.160178673319457
44	27.164929132404446	23.75029335836658	24.921947209953384	24.162830299275587
45	18.1300475713264	27.833508143405805	27.433094827883448	26.603349457384347
46	20.527744749858464	26.45553020840401	26.61135772369479	26.40536731804273
47	21.595513591251414	23.900448351687466	31.434224883240592	23.069813173820535
48	19.77952797943655	20.39482977428924	37.55921389968089	22.26642834659332
49	19.64550074465754	19.779861657199486	29.961260011723212	30.61337758641976
50	17.741201751585802	23.97674933347867	34.3494562720853	23.93259264285023
51	21.923185154453876	24.138138144818374	28.513098520584023	25.425578180143727
52	18.55671020419967	21.19209717586264	31.76311993157381	28.48807268836388
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	470.0
1	361.0
2	252.0
3	217.0
4	182.0
5	198.0
6	214.0
7	315.5
8	417.0
9	589.5
10	762.0
11	884.0
12	1006.0
13	1104.0
14	1074.5
15	947.0
16	968.0
17	989.0
18	951.0
19	913.0
20	995.0
21	1077.0
22	1587.5
23	2098.0
24	2100.0
25	2102.0
26	3057.0
27	4012.0
28	4354.5
29	4697.0
30	6823.5
31	8950.0
32	11772.0
33	14594.0
34	16038.0
35	17482.0
36	19703.0
37	21924.0
38	25306.5
39	40027.0
40	51365.0
41	66814.0
42	82263.0
43	94047.0
44	105831.0
45	120052.5
46	134274.0
47	131946.5
48	129619.0
49	108603.5
50	87588.0
51	69577.0
52	51566.0
53	45599.0
54	39632.0
55	34546.5
56	29461.0
57	24565.0
58	19669.0
59	17871.0
60	16073.0
61	13948.5
62	11824.0
63	10760.5
64	7740.5
65	5784.0
66	4614.5
67	3445.0
68	3197.5
69	2950.0
70	2558.0
71	2166.0
72	1473.0
73	780.0
74	850.0
75	920.0
76	677.5
77	435.0
78	350.0
79	265.0
80	261.5
81	258.0
82	206.0
83	154.0
84	96.0
85	38.0
86	25.5
87	13.0
88	11.5
89	9.5
90	9.0
91	6.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	899071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.441482983304926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.92083752529635	10.974327169927504
2	13.344653708604493	4.6550110115344445
3	6.504898068355629	3.403652069020733
4	3.8829808991099517	2.708997811052971
5	2.490158614485369	2.1716029550138365
6	1.7534566861600667	1.834973097217383
7	1.2801480175268096	1.562937590466377
8	0.9310738924252793	1.299144756074799
9	0.7093417878970205	1.1134775450657677
>10	4.1710518036185	14.217324970867939
>50	0.768464106153238	9.713560194356601
>100	1.2218898152610822	39.12141401411576
>500	0.01084140232747307	1.341650116963773
>1k	0.008290484132773522	3.8418666091799634
>5k	0.001913188646024659	2.0400600891421456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCTT	7726	0.859331465479367	Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	5375	0.597839325259073	No Hit
TGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	5240	0.5828238259269847	No Hit
GTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	4677	0.5202036324161273	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	4554	0.5065228441357802	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3822	0.4251054699795678	No Hit
ATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3678	0.4090889373586736	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3291	0.3660445059400203	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	2531	0.2815128059964118	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2264	0.25181548509517043	No Hit
CTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2173	0.24169392628613312	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1875	0.20854860183456034	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1863	0.20721389078281913	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	1504	0.167283785151562	No Hit
GACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1175	0.13069045714965782	No Hit
TACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1133	0.12601896846856367	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	952	0.10588707677146743	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	916	0.10188294361624388	No Hit
AACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	912	0.10143803993233015	No Hit
CCCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	910	0.10121558809037329	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8920318862470261	0.0
2	0.0	0.0	0.0	1.9357759287086338	0.0
3	0.0	0.0	0.0	6.4747945379174725	0.0
4	0.0	0.0	0.0	7.410426985188044	0.0
5	0.0	0.0	0.0	8.953130509158898	0.0
6	0.0	0.0	0.0	13.823602362883465	0.0
7	0.0	0.0	0.0	14.379175838170735	0.0
8	0.0	0.0	0.0	15.583641336446176	0.0
9	0.0	0.0	0.0	16.357662520535087	0.0
10	1.1122592097843218E-4	0.0	0.0	18.69340686108216	0.0
11	1.1122592097843218E-4	0.0	0.0	20.8828891155426	0.0
12	1.1122592097843218E-4	0.0	0.0	21.773808742579842	0.0
13	1.1122592097843218E-4	0.0	0.0	22.192908012826575	0.0
14	1.1122592097843218E-4	0.0	0.0	22.374873619547287	0.0
15	1.1122592097843218E-4	0.0	0.0	22.65772113659544	0.0
16	1.1122592097843218E-4	0.0	0.0	23.43107496515848	0.0
17	1.1122592097843218E-4	0.0	0.0	24.553789411514774	0.0
18	1.1122592097843218E-4	0.0	0.0	25.736232177436488	0.0
19	6.673555258705931E-4	0.0	0.0	26.443072905254425	0.0
20	6.673555258705931E-4	0.0	0.0	27.26692330194167	0.0
21	6.673555258705931E-4	0.0	0.0	28.40498692539299	0.0
22	6.673555258705931E-4	0.0	0.0	29.382440318951453	0.0
23	6.673555258705931E-4	0.0	0.0	30.47011860019954	0.0
24	8.898073678274575E-4	0.0	0.0	31.250813339547154	0.0
25	0.0011122592097843218	0.0	0.0	31.95353870828889	0.0
26	0.0011122592097843218	0.0	0.0	32.606657316274244	0.0
27	0.0011122592097843218	0.0	0.0	33.163343050771296	0.0
28	0.0011122592097843218	0.0	0.0	33.80500538889587	0.0
29	0.0011122592097843218	0.0	0.0	34.39038741100536	0.0
30	0.0011122592097843218	0.0	0.0	35.07542785831152	0.0
31	0.0011122592097843218	0.0	0.0	35.69495623816139	0.0
32	0.0011122592097843218	0.0	0.0	36.26243088699335	0.0
33	0.0011122592097843218	0.0	0.0	36.79486937071711	0.0
34	0.0011122592097843218	0.0	0.0	37.33008850246532	0.0
35	0.001223485130762754	0.0	0.0	38.02469437897563	0.0
36	0.001223485130762754	0.0	0.0	38.587163861363564	0.0
37	0.001223485130762754	0.0	0.0	39.15052315111932	0.0
38	0.001223485130762754	0.0	0.0	39.69797713417517	0.0
39	0.001223485130762754	0.0	0.0	40.232862588160444	0.0
40	0.001223485130762754	0.0	0.0	40.77186340122193	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	35	1.0192343E-7	46.000004	11
GTCGCGT	35	1.0192343E-7	46.000004	18
TCGCAAT	35	1.0192343E-7	46.000004	30
CGTATTG	55	1.8189894E-12	46.000004	1
GCGACGT	35	1.0192343E-7	46.000004	17
GCGACGA	35	1.0192343E-7	46.000004	10
CTGTATT	35	1.0192343E-7	46.000004	33
GGTAATC	110	0.0	46.000004	8
CGAGTCG	35	1.0192343E-7	46.000004	15
CAACGGA	55	1.8189894E-12	46.000004	37
ACTGACG	55	1.8189894E-12	46.000004	1
CGTGCCC	35	1.0192343E-7	46.000004	20
ATAATCG	35	1.0192343E-7	46.000004	27
ATTACGC	35	1.0192343E-7	46.000004	13
GCTATCA	35	1.0192343E-7	46.000004	9
CTCCCGG	35	1.0192343E-7	46.000004	23
TTCACGA	35	1.0192343E-7	46.000004	26
GCTGCGA	35	1.0192343E-7	46.000004	31
CCTACCG	35	1.0192343E-7	46.000004	38
ATTCGCT	35	1.0192343E-7	46.000004	16
>>END_MODULE