##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545566_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1238591
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.956918789172537	31.0	31.0	34.0	31.0	34.0
2	32.59189030115672	34.0	31.0	34.0	31.0	34.0
3	32.57185705370053	34.0	31.0	34.0	31.0	34.0
4	36.04143417802971	37.0	35.0	37.0	35.0	37.0
5	35.83719323004931	37.0	35.0	37.0	35.0	37.0
6	35.84831877512431	37.0	35.0	37.0	35.0	37.0
7	35.83618401877617	37.0	35.0	37.0	35.0	37.0
8	35.80418636983475	37.0	35.0	37.0	35.0	37.0
9	37.93158274200281	39.0	38.0	39.0	35.0	39.0
10	37.87060942635624	39.0	38.0	39.0	35.0	39.0
11	37.70083102493075	39.0	38.0	39.0	35.0	39.0
12	37.24804798355551	39.0	37.0	39.0	34.0	39.0
13	37.01810928708508	39.0	37.0	39.0	33.0	39.0
14	37.73822512839186	40.0	36.0	41.0	33.0	41.0
15	37.6092406613644	40.0	36.0	41.0	33.0	41.0
16	37.61280681031914	40.0	35.0	41.0	33.0	41.0
17	37.715383851489314	40.0	35.0	41.0	33.0	41.0
18	37.77890522375829	40.0	35.0	41.0	33.0	41.0
19	37.737452476241145	40.0	35.0	41.0	33.0	41.0
20	37.690807538566	40.0	35.0	41.0	33.0	41.0
21	37.66905782457648	40.0	35.0	41.0	33.0	41.0
22	37.58464900842974	40.0	35.0	41.0	33.0	41.0
23	37.495432309777804	39.0	35.0	41.0	33.0	41.0
24	37.43466002901684	39.0	35.0	41.0	32.0	41.0
25	37.38107091041353	39.0	35.0	41.0	32.0	41.0
26	37.28202045711619	39.0	35.0	41.0	32.0	41.0
27	37.173396221997415	39.0	35.0	41.0	32.0	41.0
28	37.07379917987455	39.0	35.0	41.0	32.0	41.0
29	37.00453499177695	39.0	35.0	41.0	32.0	41.0
30	36.9138432299282	39.0	35.0	41.0	31.0	41.0
31	36.818894211244874	39.0	35.0	41.0	31.0	41.0
32	36.706820895679044	39.0	35.0	41.0	31.0	41.0
33	36.62128660712051	39.0	35.0	41.0	31.0	41.0
34	36.502191603200735	39.0	35.0	41.0	31.0	41.0
35	36.423690306162406	38.0	35.0	41.0	30.0	41.0
36	36.313575667835465	38.0	35.0	41.0	30.0	41.0
37	36.165223225423084	38.0	35.0	41.0	30.0	41.0
38	36.07684134633628	38.0	35.0	41.0	30.0	41.0
39	35.99810268280651	38.0	35.0	40.0	30.0	41.0
40	35.89180528519907	38.0	35.0	40.0	30.0	41.0
41	35.84083688642982	38.0	35.0	40.0	29.0	41.0
42	35.698094851327035	38.0	35.0	40.0	29.0	41.0
43	35.60163120836499	37.0	35.0	40.0	29.0	41.0
44	35.47905482923741	37.0	34.0	40.0	29.0	41.0
45	35.46077518728943	37.0	34.0	40.0	29.0	41.0
46	35.418000776689	37.0	34.0	40.0	29.0	41.0
47	35.33312933809466	37.0	34.0	40.0	28.0	41.0
48	35.25287443554814	37.0	34.0	40.0	28.0	41.0
49	35.194104429953065	36.0	34.0	40.0	28.0	41.0
50	35.10382684841082	36.0	34.0	40.0	28.0	41.0
51	34.936550483573676	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	14.0
11	15.0
12	18.0
13	28.0
14	34.0
15	47.0
16	90.0
17	160.0
18	308.0
19	740.0
20	1102.0
21	1867.0
22	2974.0
23	4470.0
24	6493.0
25	9318.0
26	12461.0
27	15090.0
28	17339.0
29	20060.0
30	24251.0
31	29849.0
32	36853.0
33	48181.0
34	78991.0
35	155448.0
36	92301.0
37	125111.0
38	186849.0
39	367911.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.1570478067416925	90.95932394147866	3.1744942438625827	3.709134007917061
2	3.7138974851262443	2.7169582210753997	90.8080229874107	2.7611213063876616
3	28.77277487080077	15.435280895792072	42.131583387898026	13.660360845509132
4	26.719473982937064	15.853094362868775	43.950989471100634	13.476442183093532
5	25.020123672786255	14.382956117071737	46.19571755325204	14.401202656889966
6	23.204673697774325	14.689594870300205	47.30996753569177	14.795763896233705
7	22.338447477819553	17.644726951834784	45.44405699702323	14.572768573322428
8	26.1513284046146	19.09750676373395	43.70934392386187	11.041820907789578
9	82.53491265478273	4.401614415089404	9.176556264335845	3.8869166657920164
10	83.27841878392464	4.364394703336291	8.830679376808002	3.52650713593107
11	77.3310156460042	6.712788967463836	10.22718556811732	5.729009818414634
12	44.09793063246867	22.802523189656636	16.080530215381835	17.019015962492865
13	35.93324995902602	25.066708865154038	21.794845917659664	17.20519525816028
14	30.858209045601008	23.736406933362183	25.663435306731603	19.741948714305206
15	23.034883993182575	27.06962992626299	30.43668168103918	19.458804399515255
16	18.818964452349483	33.12990325297051	28.806603632676165	19.24452866200384
17	18.082805381275982	24.029401150177904	38.52474303462564	19.363050433920478
18	21.544319311217343	20.776188427011014	36.580840648769446	21.098651613002193
19	22.73155545292998	19.676471086904392	29.516200263040826	28.075773197124796
20	23.498394546706702	22.38043066678185	30.966800178590027	23.154374607921422
21	27.64383077222424	24.006794817659742	26.644711611823436	21.704662798292578
22	29.238707531380413	22.51873297965188	26.327092639943288	21.915466849024416
23	27.16643347158182	23.9508441446773	27.77389792110551	21.108824462635365
24	29.22587036398617	20.67954635549588	24.94423098504672	25.150352295471222
25	26.858906612432996	24.53279573321621	24.61886127058892	23.989436383761873
26	26.08084508929905	21.867266918619624	30.462840437238764	21.58904755484256
27	26.897902536026823	22.55466090097538	27.762594754846436	22.78484180815136
28	23.423389964887523	27.164253575231857	26.46571790042072	22.946638559459902
29	23.047156002263865	27.266062808465424	26.504148665701592	23.18263252356912
30	22.222751497467687	25.95328078437515	30.525976694485912	21.297991023671255
31	22.651625920097917	24.761361902355176	28.471464753094445	24.115547424452462
32	21.9622135151959	27.77769255549249	28.256462383466374	22.00363154584524
33	27.06720781920747	25.292126295120827	25.382147940684213	22.258517944987492
34	27.36730688338604	25.29802008895592	25.702673441030978	21.631999586627064
35	27.19549875624803	26.292456509049394	23.309147248768962	23.20289748593361
36	24.961185734435336	25.539827109998374	25.646803504950384	23.852183650615903
37	23.61457494846967	25.179175369431878	28.618728862069887	22.587520820028566
38	27.00730103803435	25.917595073757198	24.227771718024755	22.8473321701837
39	25.48662149167885	27.973237331774577	25.242634574286427	21.29750660226015
40	25.366404244823354	26.940773830909475	25.437533455353705	22.255288468913466
41	24.161163773998034	26.580606511754084	24.681997527836067	24.57623218641182
42	25.88126346792444	23.813833622236878	27.27978808178002	23.02511482805866
43	22.793480656649372	24.41637312074769	27.622354756331998	25.16779146627095
44	23.02584146017531	25.702511967227277	25.759027798522677	25.512618774074735
45	22.989671328146258	24.426465233479007	26.97444111898116	25.60942231939357
46	24.780092863584507	24.042480528277697	27.217701404256932	23.95972520388086
47	22.505815075355788	26.908075385659995	26.053878964080962	24.53223057490326
48	23.191109898263427	26.587065463902128	24.846135649298276	25.375688988536165
49	23.20661138341874	25.699040280447704	27.760899279907576	23.333449056225987
50	23.091076876870574	23.012116186860716	30.511282578349107	23.385524357919603
51	22.95051393074873	24.391506154977712	27.20470276305899	25.453277151214564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	533.0
1	1238.0
2	1943.0
3	4018.0
4	6093.0
5	4379.0
6	2665.0
7	2593.0
8	2521.0
9	2640.5
10	2760.0
11	3038.5
12	3317.0
13	3282.0
14	3247.0
15	3039.0
16	2831.0
17	3275.5
18	3720.0
19	3798.0
20	3876.0
21	4562.5
22	5249.0
23	6184.0
24	7119.0
25	8280.0
26	10161.0
27	10881.0
28	12169.5
29	13458.0
30	16313.5
31	19169.0
32	23633.5
33	28098.0
34	28954.5
35	29811.0
36	34719.0
37	39627.0
38	46912.5
39	54198.0
40	65297.0
41	76396.0
42	87894.5
43	99393.0
44	108940.0
45	118487.0
46	114263.5
47	110040.0
48	96685.0
49	83330.0
50	80622.0
51	77914.0
52	72614.0
53	67314.0
54	66069.5
55	64825.0
56	67591.5
57	70358.0
58	66250.5
59	62143.0
60	55430.5
61	48718.0
62	41704.0
63	34690.0
64	29131.0
65	23572.0
66	18843.0
67	14114.0
68	13751.5
69	13389.0
70	11476.5
71	9564.0
72	8054.5
73	6545.0
74	4957.0
75	2815.0
76	2261.0
77	1668.0
78	1075.0
79	713.0
80	351.0
81	233.5
82	116.0
83	71.5
84	27.0
85	22.0
86	17.0
87	15.5
88	14.0
89	9.0
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1238591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.787811381673924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07743924303033	11.695206384063727
2	10.886188485858806	3.4373818096017854
3	4.182758485545171	1.981098060746497
4	2.145253156545413	1.3547540880591833
5	1.2360701481592224	0.9757421176827772
6	0.7528847285761371	0.7131841252141693
7	0.5170904510451036	0.5714608555855353
8	0.37172273175668963	0.4694950700204151
9	0.2965171066585	0.4213220536227678
>10	2.5060913488545253	8.993986829206312
>50	1.1261309946060185	13.265266184909526
>100	1.8874878948314948	53.78818716924339
>500	0.010260874666113046	1.093073096532628
>1k	0.004104349866445219	1.2398421555112957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	3348	0.27030714739571016	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3257	0.2629600893273082	No Hit
ATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	2014	0.16260412032704905	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	1673	0.1350728367960045	No Hit
ATGCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	1355	0.10939850200752306	No Hit
ATGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT	1249	0.1008403904113626	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14742558277914178	0.0
2	0.0	0.0	0.0	0.5656427343650972	0.0
3	0.0	0.0	0.0	0.792190480957798	0.0
4	8.073690185057052E-5	0.0	0.0	1.0352085555280153	0.0
5	8.073690185057052E-5	0.0	0.0	1.8975594041939592	0.0
6	8.073690185057052E-5	0.0	0.0	2.097948394587075	0.0
7	8.073690185057052E-5	0.0	0.0	2.4755548845421935	0.0
8	8.073690185057052E-5	0.0	0.0	3.5807623339746533	0.0
9	8.073690185057052E-5	0.0	0.0	3.715512223163256	0.0
10	8.073690185057052E-5	0.0	0.0	3.9890488466329885	0.0
11	8.073690185057052E-5	0.0	0.0	4.192021417885323	0.0
12	8.073690185057052E-5	0.0	0.0	4.822334410632727	0.0
13	1.6147380370114104E-4	0.0	0.0	5.3480123785817915	0.0
14	1.6147380370114104E-4	0.0	0.0	5.57843549646332	0.0
15	1.6147380370114104E-4	0.0	0.0	5.705353906172417	0.0
16	2.422107055517116E-4	0.0	0.0	5.7831035426545165	0.0
17	2.422107055517116E-4	0.0	0.0	5.901544577669303	0.0
18	2.422107055517116E-4	0.0	0.0	6.15731908273191	0.0
19	2.422107055517116E-4	0.0	0.0	6.458144779027137	0.0
20	3.229476074022821E-4	0.0	0.0	6.826950946680543	0.0
21	4.0368450925285264E-4	0.0	0.0	7.040419315173451	0.0
22	4.0368450925285264E-4	0.0	0.0	7.28513286468253	0.0
23	4.0368450925285264E-4	0.0	0.0	7.673638836387476	0.0
24	4.0368450925285264E-4	0.0	0.0	7.9969093913971605	0.0
25	4.844214111034232E-4	0.0	0.0	8.345935018097176	0.0
26	0.0025028439573676863	0.0	0.0	8.623185539052036	0.0
27	0.0025835808592182567	0.0	0.0	8.869190878990723	0.0
28	0.0026643177610688276	0.0	0.0	9.10211684082962	0.0
29	0.0026643177610688276	0.0	0.0	9.30735004533377	0.0
30	0.0026643177610688276	0.0	0.0	9.53236379079131	0.0
31	0.002906528466620539	0.0	0.0	9.757781220758103	0.0
32	0.002906528466620539	0.0	0.0	10.01202172468555	0.0
33	0.002906528466620539	0.0	0.0	10.270944968920329	0.0
34	0.0029872653684711093	0.0	0.0	10.533501373738385	0.0
35	0.0029872653684711093	0.0	0.0	10.803404836624843	0.0
36	0.0029872653684711093	0.0	0.0	11.053124074048657	0.0
37	0.0029872653684711093	0.0	0.0	11.341274076753344	0.0
38	0.00306800227032168	0.0	0.0	11.604799324393605	0.0
39	0.00306800227032168	0.0	0.0	11.871634785009741	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.033498E-4	45.000004	13
CCTTACG	20	7.033498E-4	45.000004	27
ACACGGT	20	7.033498E-4	45.000004	30
CCCTCGA	20	7.033498E-4	45.000004	22
CGTGCTA	20	7.033498E-4	45.000004	37
GCAACTA	20	7.033498E-4	45.000004	43
GACGTAA	20	7.033498E-4	45.000004	18
ATTCGTC	40	6.8157533E-9	45.000004	1
CGACACG	20	7.033498E-4	45.000004	36
TAGCCCG	20	7.033498E-4	45.000004	26
CTAACGG	20	7.033498E-4	45.000004	4
CCGTAGA	20	7.033498E-4	45.000004	3
TACGCAC	20	7.033498E-4	45.000004	25
CTACGTC	20	7.033498E-4	45.000004	36
CTAGTAA	20	7.033498E-4	45.000004	39
CTACGGT	20	7.033498E-4	45.000004	5
GGACGTA	20	7.033498E-4	45.000004	17
CCGACAC	20	7.033498E-4	45.000004	37
CGTCGGT	40	6.8157533E-9	45.000004	22
CGAGAAT	40	6.8157533E-9	45.000004	40
>>END_MODULE