FastQCFastQC Report
Thu 26 May 2016
SRR1545565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545565_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences972754
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT33660.3460278754957574Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC23830.24497457733404335No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC20290.20858305388618295No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT18200.18709766292402807No Hit
TGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC17010.1748643541943801No Hit
TTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC15830.1627338463784266No Hit
ATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC14180.14577169561883066No Hit
GGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC10660.10958577399835931No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG253.4167657E-546.020
TCACGAT206.310975E-446.045
CAGGTCG253.4167657E-546.040
AACGTCC301.8611063E-646.022
AGCGTAA301.8611063E-646.036
AATCCGA253.4167657E-546.031
GGTACGC253.4167657E-546.024
CGTATTG301.8611063E-646.01
CCCTCGA206.310975E-446.020
CTCCGTT301.8611063E-646.026
GATCGGC405.6097633E-946.038
ATCTAAC405.6097633E-946.028
GTACACG301.8611063E-646.022
GATCGAC253.4167657E-546.037
AAATACG206.310975E-446.018
CCTAGTG206.310975E-446.025
CGAAACA253.4167657E-546.042
AACCCGA453.092282E-1046.024
GTCCTAT206.310975E-446.037
TTTCGAA206.310975E-446.029
Produced by FastQC (version 0.11.5)