FastQCFastQC Report
Thu 26 May 2016
SRR1545564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545564_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2810815
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113290.4030503608384045No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC81170.28877745422590956No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30900.10993252846594316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG600.045.0000041
ACTACGC302.1666256E-645.00000410
CATACGT453.8562575E-1045.00000415
TACGTCC302.1666256E-645.00000416
TAATGCG1400.045.01
CTAACGC253.8922517E-544.99999620
ATCGGGT1000.042.7499964
TACAAGG6100.042.0491832
CGGTCTA2800.041.78571331
TCACGAC2950.041.18643625
TACGCGG1150.041.0869562
CTCACGA3000.040.50000424
CGTATGG2800.040.178572
GATCGAC900.040.00000410
ATTACGC451.9299478E-840.00000412
AGGGCGA19700.039.8604056
ACGGTCT3000.039.75000430
ATACTAT21250.039.60000245
TAGGGCG9400.039.4946825
ATAGCGG6100.039.4672132
Produced by FastQC (version 0.11.5)