##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545564_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2810815
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26052977517197	31.0	31.0	33.0	30.0	34.0
2	31.600074711427112	31.0	31.0	34.0	30.0	34.0
3	31.70147056992367	31.0	31.0	34.0	30.0	34.0
4	35.40902656347003	37.0	35.0	37.0	33.0	37.0
5	35.2704870295626	37.0	35.0	37.0	33.0	37.0
6	35.34192075963733	37.0	35.0	37.0	32.0	37.0
7	35.8081485263171	37.0	35.0	37.0	35.0	37.0
8	35.826122672605635	37.0	35.0	37.0	35.0	37.0
9	37.49069682636531	39.0	37.0	39.0	35.0	39.0
10	36.845785652915616	39.0	37.0	39.0	32.0	39.0
11	36.50022360062829	39.0	35.0	39.0	32.0	39.0
12	35.778048003870765	37.0	35.0	39.0	31.0	39.0
13	35.45961082461848	37.0	35.0	39.0	30.0	39.0
14	36.41333492243353	38.0	35.0	41.0	31.0	41.0
15	36.61618462972483	38.0	35.0	41.0	31.0	41.0
16	36.76637914626185	38.0	35.0	41.0	32.0	41.0
17	36.701084916652285	38.0	35.0	40.0	31.0	41.0
18	36.640243843867346	38.0	35.0	40.0	31.0	41.0
19	36.604217282176165	38.0	35.0	40.0	31.0	41.0
20	36.46798597559783	38.0	35.0	40.0	31.0	41.0
21	36.278543767554964	38.0	35.0	40.0	31.0	41.0
22	36.20699690303346	38.0	35.0	40.0	30.0	41.0
23	36.127356656343444	38.0	34.0	40.0	30.0	41.0
24	36.08364726956417	37.0	34.0	40.0	30.0	41.0
25	35.9519039851431	37.0	34.0	40.0	30.0	41.0
26	35.86672762170402	37.0	34.0	40.0	30.0	41.0
27	35.80219117942661	37.0	34.0	40.0	30.0	41.0
28	35.77744390861725	37.0	34.0	40.0	30.0	41.0
29	35.76033072258402	37.0	34.0	40.0	30.0	41.0
30	35.664385952117094	37.0	34.0	40.0	29.0	41.0
31	35.5126865339768	37.0	34.0	40.0	29.0	41.0
32	35.352823291465285	37.0	34.0	40.0	29.0	41.0
33	35.198036512541734	37.0	34.0	40.0	27.0	41.0
34	35.0219907749176	37.0	34.0	40.0	27.0	41.0
35	34.88495863299435	36.0	34.0	40.0	26.0	41.0
36	34.7071230230378	36.0	33.0	40.0	25.0	41.0
37	34.6326606340154	36.0	33.0	40.0	25.0	41.0
38	34.609713197062064	36.0	33.0	40.0	25.0	41.0
39	34.531309958143815	36.0	33.0	40.0	25.0	41.0
40	34.354024722367	36.0	33.0	40.0	24.0	41.0
41	34.31979692722573	36.0	33.0	40.0	24.0	41.0
42	34.237778011003925	36.0	33.0	40.0	24.0	41.0
43	34.15811072589267	35.0	33.0	40.0	23.0	41.0
44	34.10415093131352	35.0	33.0	40.0	23.0	41.0
45	34.065939949801034	35.0	33.0	40.0	23.0	41.0
46	34.03641008035036	35.0	33.0	40.0	23.0	41.0
47	33.98066895188762	35.0	33.0	40.0	23.0	41.0
48	33.918126948945414	35.0	33.0	40.0	23.0	41.0
49	33.84480337553343	35.0	33.0	39.0	24.0	41.0
50	33.73517929853085	35.0	33.0	39.0	24.0	41.0
51	33.57382574093279	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	5.0
14	40.0
15	104.0
16	291.0
17	794.0
18	1778.0
19	3489.0
20	5587.0
21	9122.0
22	13376.0
23	19090.0
24	26272.0
25	35896.0
26	46515.0
27	55046.0
28	61883.0
29	71293.0
30	84409.0
31	101602.0
32	124541.0
33	159170.0
34	259683.0
35	384563.0
36	207417.0
37	250217.0
38	342430.0
39	545851.0
40	346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.74973806529423	22.963766736693806	27.346125589908976	13.940369608102987
2	31.79131319563899	25.053516506778283	29.637489482587792	13.51768081499494
3	29.90360447058949	25.022884821662046	31.124673804572694	13.948836903175769
4	26.857584010331525	27.3727726655792	30.020261027495582	15.749382296593692
5	23.641043611906156	32.36513253273517	29.03069038695183	14.96313346840685
6	21.081963772073227	41.29916056375108	27.144333582964368	10.474542081211322
7	85.81479037218742	5.104533738435293	7.102601914391378	1.9780739749859029
8	86.42813561191328	4.232509076548972	7.089651933691829	2.249703377845927
9	82.1739602215016	6.0144833437988625	8.46352392455569	3.348032510143855
10	47.08620097729662	25.413305393631386	14.906210476320926	12.594283152751071
11	40.56542319576351	22.923671604143284	21.29232980470077	15.218575395392438
12	37.55110172672339	21.734585876338357	25.47172261425957	15.242589782678689
13	22.999663798577995	35.53318165727733	25.715637635347754	15.751516908796914
14	17.74115336655027	38.64953758963148	28.22900119716168	15.380307846656574
15	16.8926805926395	24.563836467359113	42.70668827368574	15.836794666315642
16	18.48873013698874	20.369038873067062	41.59729473480112	19.54493625514308
17	18.75239032095673	20.240713102783356	29.604011647867257	31.402884928392655
18	21.385114281800828	24.36218676789472	33.849577435725934	20.403121514578512
19	28.246255979137725	25.02480597264494	26.43251156692988	20.296426481287455
20	30.13645508509098	23.431282386069523	26.004770858274203	20.4274916705653
21	23.472907324032356	27.296922778624705	28.14553786001569	21.084632037327253
22	22.878738017265455	24.76381405393098	25.819201904074085	26.538246024729485
23	20.862454483841873	29.31697746027398	25.571729195980524	24.248838859903625
24	20.43297050855357	24.85837737453372	36.080140457482976	18.62851165942974
25	20.187383374572857	25.332190129908945	33.42066269035849	21.059763805159715
26	19.375661507427562	31.47752520176532	27.28343914487435	21.863374145932763
27	19.14362916093731	32.45937566150743	27.780732634485016	20.61626254307025
28	17.34802895245685	28.35629523821383	35.011019935499135	19.28465587383019
29	18.24666511314334	25.300846907391627	33.88867641591496	22.563811563550075
30	19.61612557212054	30.314908665280356	30.495888203243542	19.57307755935556
31	27.189587361672686	27.61017711944756	25.26060946736089	19.939626051518864
32	28.217758906224706	26.193719615129417	26.341292472112183	19.247229006533694
33	26.606518038362537	27.77653456381868	25.44286977264601	20.17407762517277
34	20.25714961674817	27.308271800171834	28.05414088084773	24.38043770223227
35	20.844381433854593	26.078699594245798	30.378413378326215	22.698505593573394
36	28.67979571761215	26.467056707752022	25.744170285130824	19.108977289505003
37	21.29770191207888	32.358372927424966	27.135012443010304	19.208912717485855
38	21.528951567427953	32.47666602035353	24.392391530570315	21.601990881648206
39	21.68840709900865	30.85236844118165	26.105382246786075	21.353842213023626
40	23.98169214267036	26.62644108559261	26.921871414518566	22.46999535721846
41	18.89146030599666	25.817849983012042	27.35391692445074	27.93677278654056
42	21.744582976823448	26.92382814237152	25.474426456383647	25.857162424421386
43	21.749101239320268	25.92981039307105	27.219649816868063	25.101438550740625
44	19.766331117487276	28.646211152281452	29.12870466394978	22.458753066281485
45	18.997692839977017	33.93883980269068	24.963364718062202	22.100102639270105
46	21.530018873529563	32.46336027095344	26.56460848543928	19.44201237007772
47	21.879348160586876	28.39525191092263	27.062898127411444	22.662501801079046
48	22.23113225167789	25.987871844998693	29.592342434489645	22.18865346883377
49	21.023155205874453	25.94457479414334	31.498302093876685	21.533967906105524
50	20.095132550523605	31.214007325277542	27.411017800886935	21.27984232331192
51	19.667462995608034	31.758831513279954	25.85840761487327	22.71529787623874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2505.0
1	4514.5
2	6524.0
3	13946.5
4	21369.0
5	16099.5
6	10830.0
7	11150.0
8	11470.0
9	12381.0
10	13292.0
11	13300.0
12	13308.0
13	13587.5
14	13867.0
15	13801.5
16	13736.0
17	13135.5
18	12535.0
19	12449.5
20	12364.0
21	12869.5
22	13375.0
23	14228.5
24	15082.0
25	16329.5
26	22495.5
27	27414.0
28	30627.5
29	33841.0
30	39949.0
31	46057.0
32	52609.5
33	59162.0
34	70348.5
35	81535.0
36	85005.0
37	88475.0
38	99713.5
39	110952.0
40	139170.0
41	167388.0
42	202103.5
43	236819.0
44	246276.0
45	255733.0
46	246651.5
47	237570.0
48	229555.5
49	221541.0
50	209384.5
51	197228.0
52	183879.0
53	170530.0
54	156264.5
55	141999.0
56	132057.5
57	122116.0
58	116334.0
59	110552.0
60	99367.0
61	88182.0
62	78725.5
63	69269.0
64	60797.5
65	52326.0
66	45062.5
67	37799.0
68	31875.5
69	25952.0
70	22839.5
71	19727.0
72	16452.5
73	13178.0
74	10723.0
75	6365.5
76	4463.0
77	3785.0
78	3107.0
79	1988.5
80	870.0
81	714.0
82	558.0
83	391.0
84	224.0
85	159.0
86	94.0
87	66.0
88	38.0
89	22.0
90	6.0
91	5.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2810815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.994157137472017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34315780438833	12.850210908440985
2	7.489681055792473	2.395822714317843
3	2.572750240486604	1.2344691486543502
4	1.3969841649295378	0.8937433700977262
5	0.8970130607150512	0.7173483923720628
6	0.6684817833262054	0.6415081611634111
7	0.46983702500121355	0.5260253044810246
8	0.3703657740709932	0.473895071106634
9	0.27899754637740837	0.40160975379564656
>10	2.6220732108450386	9.887969832086728
>50	1.1780607699158676	13.794104213881967
>100	1.69240712930195	52.911089578701095
>500	0.015703671549480956	1.6639095493871554
>1k	0.0040380869698665315	0.9105521021887272
>5k	2.2433816499258508E-4	0.29124606586538593
>10k+	2.2433816499258508E-4	0.40649583345927764
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11329	0.4030503608384045	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8117	0.28877745422590956	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3090	0.10993252846594316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05048357860620496	0.0
2	0.0	0.0	0.0	0.1578189955582278	0.0
3	0.0	0.0	0.0	0.21502660260458265	0.0
4	0.0	0.0	0.0	0.3281254725053054	0.0
5	0.0	0.0	0.0	0.529739595099642	0.0
6	3.5576870053703284E-5	0.0	0.0	0.7219614239998008	0.0
7	3.5576870053703284E-5	0.0	0.0	0.8349535632903624	0.0
8	3.5576870053703284E-5	0.0	0.0	1.0782637775876391	0.0
9	3.5576870053703284E-5	0.0	0.0	1.1588098113892233	0.0
10	3.5576870053703284E-5	0.0	0.0	1.308481703705153	0.0
11	3.5576870053703284E-5	0.0	0.0	1.4983198823117139	0.0
12	3.5576870053703284E-5	0.0	0.0	1.6543956112373102	0.0
13	3.5576870053703284E-5	0.0	0.0	1.7154810971195187	0.0
14	3.5576870053703284E-5	0.0	0.0	1.7363291429709888	0.0
15	3.5576870053703284E-5	0.0	0.0	1.77457427827872	0.0
16	3.5576870053703284E-5	0.0	0.0	1.8625914547915818	0.0
17	3.5576870053703284E-5	0.0	0.0	1.9700691792238194	0.0
18	7.115374010740657E-5	0.0	0.0	2.116041077054164	0.0
19	7.115374010740657E-5	0.0	0.0	2.1888669300540946	0.0
20	1.0673061016110986E-4	0.0	0.0	2.2608389381727365	0.0
21	1.4230748021481314E-4	0.0	0.0	2.356825333577628	0.0
22	1.4230748021481314E-4	0.0	0.0	2.461243447185247	0.0
23	1.4230748021481314E-4	0.0	0.0	2.583094227119181	0.0
24	1.4230748021481314E-4	0.0	0.0	2.680752735416596	0.0
25	1.4230748021481314E-4	0.0	0.0	2.7554285856593195	0.0
26	1.4230748021481314E-4	0.0	0.0	2.832701547415963	0.0
27	1.4230748021481314E-4	0.0	0.0	2.908587011240512	0.0
28	1.7788435026851643E-4	0.0	0.0	2.993224385098272	0.0
29	2.1346122032221972E-4	0.0	0.0	3.0875386676106396	0.0
30	2.49038090375923E-4	0.0	0.0	3.199001001488892	0.0
31	2.49038090375923E-4	0.0	0.0	3.305696034779948	0.0
32	2.846149604296263E-4	0.0	0.0	3.4074103062634857	0.0
33	2.846149604296263E-4	0.0	0.0	3.5093736158373994	0.0
34	2.846149604296263E-4	0.0	0.0	3.6154994192075964	0.0
35	2.846149604296263E-4	0.0	0.0	3.7422242303388873	0.0
36	2.846149604296263E-4	0.0	0.0	3.855109639019288	0.0
37	3.2019183048332956E-4	0.0	0.0	3.9685998544906016	0.0
38	3.2019183048332956E-4	0.0	0.0	4.080524687679552	0.0
39	3.2019183048332956E-4	0.0	0.0	4.206609115149877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	60	0.0	45.000004	1
ACTACGC	30	2.1666256E-6	45.000004	10
CATACGT	45	3.8562575E-10	45.000004	15
TACGTCC	30	2.1666256E-6	45.000004	16
TAATGCG	140	0.0	45.0	1
CTAACGC	25	3.8922517E-5	44.999996	20
ATCGGGT	100	0.0	42.749996	4
TACAAGG	610	0.0	42.049183	2
CGGTCTA	280	0.0	41.785713	31
TCACGAC	295	0.0	41.186436	25
TACGCGG	115	0.0	41.086956	2
CTCACGA	300	0.0	40.500004	24
CGTATGG	280	0.0	40.17857	2
GATCGAC	90	0.0	40.000004	10
ATTACGC	45	1.9299478E-8	40.000004	12
AGGGCGA	1970	0.0	39.860405	6
ACGGTCT	300	0.0	39.750004	30
ATACTAT	2125	0.0	39.600002	45
TAGGGCG	940	0.0	39.494682	5
ATAGCGG	610	0.0	39.467213	2
>>END_MODULE