FastQCFastQC Report
Thu 26 May 2016
SRR1545563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545563_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2419893
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113610.4694835680751174No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45760.18909927009169414No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT31630.13070825858829296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG453.8562575E-1045.0000041
TATCTCG502.1827873E-1145.00000428
CGCGAAA207.034834E-445.027
CGGTCTA3750.042.60000231
TAATGCG900.042.5000041
CGTTTTT78900.041.6920171
GCGTAAG1200.041.2499961
GTATGCG1150.041.086961
TACGGGA6900.040.760874
TAGGGTA14650.040.3924945
TAGGGAC25750.039.9320375
AGGGTAT14100.039.734046
TATCTAG1650.039.5454521
ATAGCGG4100.039.5121962
CGAGGGA11450.039.4978144
CTCACGA4050.039.44444324
TCGATTG1550.039.1935461
ATACTAT36200.039.1574645
TCACGAC4150.039.03614425
ACGTTAG1500.039.01
Produced by FastQC (version 0.11.5)