FastQCFastQC Report
Thu 26 May 2016
SRR1545562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545562_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1652823
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.4452987403974896No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44880.2715354275684692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1550.045.025
GATACGA207.0341857E-445.011
GGTACGA502.1827873E-1145.037
GTACCGG453.8562575E-1045.02
CGTGCAA207.0341857E-445.018
ATTCGCT207.0341857E-445.014
ATATCGC207.0341857E-445.029
CGACTAT253.8915503E-545.021
TATCGCC253.8915503E-545.030
TATCGAT551.8189894E-1245.013
GCGTACT253.8915503E-545.021
ATATTAC207.0341857E-445.042
GATCGAC302.1660817E-644.99999622
AACGTAG1200.043.1249961
TCGTTGA1850.042.56756624
CTAACGG850.042.352942
CGGGTAT1500.042.06
GCGATAT2200.041.931829
TACGGGA3500.041.7857134
TCGGGCA650.041.538465
Produced by FastQC (version 0.11.5)