FastQCFastQC Report
Thu 26 May 2016
SRR1545561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545561_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1385305
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52370.37803949310801593No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47120.3401417016469297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG302.1658252E-645.00000418
ACGTTAG351.2121745E-745.0000041
GACGTAT302.1658252E-645.00000411
TACGACT302.1658252E-645.00000421
TAGGCCC302.1658252E-645.00000435
CGACTCA302.1658252E-645.00000423
GGTTCGA302.1658252E-645.00000438
TACGTAA351.2121745E-745.00000418
ACACGGC551.8189894E-1245.031
GCGCGTT207.03379E-445.025
CGCATCG207.03379E-445.021
TTCGTAA207.03379E-445.032
ACTTACG406.8175723E-945.01
ATCCGTT253.891222E-545.016
ATATCCG207.03379E-445.02
CGTAGTG253.891222E-545.045
ACAATCG453.8562575E-1045.023
CGAATAC207.03379E-445.027
CGTACTA253.891222E-545.026
AGCGAAT207.03379E-445.025