##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545561_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1385305
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325926781466897	31.0	31.0	33.0	30.0	34.0
2	31.667956154059937	31.0	31.0	34.0	30.0	34.0
3	31.795260249547933	31.0	31.0	34.0	30.0	34.0
4	35.500874536654386	37.0	35.0	37.0	33.0	37.0
5	35.374597651780654	37.0	35.0	37.0	33.0	37.0
6	35.44693984357235	37.0	35.0	37.0	33.0	37.0
7	35.827456769447885	37.0	35.0	37.0	35.0	37.0
8	35.83631763402283	37.0	35.0	37.0	35.0	37.0
9	37.519653072789026	39.0	37.0	39.0	35.0	39.0
10	36.935360805021276	39.0	37.0	39.0	32.0	39.0
11	36.55715672721892	39.0	35.0	39.0	32.0	39.0
12	35.675441870201865	37.0	35.0	39.0	31.0	39.0
13	35.316523076145685	37.0	35.0	39.0	30.0	39.0
14	36.28481092611374	38.0	35.0	40.0	31.0	41.0
15	36.548259047646546	38.0	35.0	40.0	31.0	41.0
16	36.72958879091608	38.0	35.0	41.0	32.0	41.0
17	36.67500369954631	38.0	35.0	40.0	32.0	41.0
18	36.611663135554984	38.0	35.0	40.0	31.0	41.0
19	36.56661890341838	37.0	35.0	40.0	31.0	41.0
20	36.40708869165996	37.0	35.0	40.0	31.0	41.0
21	36.201115999725694	37.0	35.0	40.0	31.0	41.0
22	36.11522155770751	37.0	35.0	40.0	30.0	41.0
23	36.09350431854357	37.0	35.0	40.0	31.0	41.0
24	36.0150053598305	37.0	34.0	40.0	31.0	41.0
25	35.897623267078366	37.0	34.0	40.0	30.0	41.0
26	35.80619502564417	37.0	34.0	40.0	30.0	41.0
27	35.74980527753816	37.0	34.0	40.0	30.0	41.0
28	35.73003923323745	36.0	34.0	40.0	30.0	41.0
29	35.75387802686051	36.0	34.0	40.0	30.0	41.0
30	35.669360898863424	36.0	34.0	40.0	30.0	41.0
31	35.488005890399585	36.0	34.0	40.0	29.0	41.0
32	35.31386012466569	36.0	34.0	40.0	29.0	41.0
33	35.16901620942681	36.0	34.0	40.0	28.0	41.0
34	35.01479962896258	36.0	34.0	40.0	27.0	41.0
35	34.85878994156521	36.0	34.0	40.0	27.0	41.0
36	34.683016375455225	36.0	33.0	40.0	25.0	41.0
37	34.61484799376311	36.0	33.0	40.0	25.0	41.0
38	34.642278054291296	35.0	33.0	40.0	26.0	41.0
39	34.59086266201306	36.0	33.0	40.0	25.0	41.0
40	34.38937995603856	36.0	33.0	40.0	24.0	41.0
41	34.362821905645326	35.0	33.0	40.0	24.0	41.0
42	34.322515258372704	35.0	33.0	40.0	24.0	41.0
43	34.24991608346176	35.0	33.0	40.0	24.0	41.0
44	34.19089153652084	35.0	33.0	40.0	24.0	41.0
45	34.14017418546818	35.0	33.0	40.0	24.0	41.0
46	34.1255326444357	35.0	33.0	40.0	24.0	41.0
47	34.072530597954966	35.0	33.0	40.0	24.0	41.0
48	34.033380374718924	35.0	33.0	40.0	24.0	41.0
49	33.986546644962665	35.0	33.0	39.0	24.0	41.0
50	33.84849834512977	35.0	33.0	39.0	24.0	41.0
51	33.679152244451586	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	5.0
13	6.0
14	7.0
15	38.0
16	128.0
17	357.0
18	771.0
19	1406.0
20	2596.0
21	3994.0
22	6138.0
23	8484.0
24	12446.0
25	17429.0
26	22838.0
27	26850.0
28	29758.0
29	34083.0
30	40488.0
31	48776.0
32	61317.0
33	79159.0
34	137826.0
35	209526.0
36	94118.0
37	113179.0
38	160339.0
39	273081.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.22488188521661	23.563258632575497	28.225120099905798	13.986739382302094
2	31.87074326592339	24.825218995094943	29.905977384041783	13.398060354939886
3	30.253843016519827	24.616889421463142	30.738212884527233	14.391054677489794
4	25.951108239701725	28.223459815708452	30.294844817567252	15.530587127022569
5	23.258632575497813	33.337351702332704	28.802971186850552	14.601044535318938
6	21.51150829600702	41.812669412151116	26.181093694168432	10.494728597673436
7	86.55494638364837	4.620931852552326	6.837555628543894	1.9865661352554131
8	87.67332825623238	3.697380721213018	6.7657302904414545	1.8635607321131447
9	82.99450301558142	5.721411530312819	8.158347800664835	3.1257376534409387
10	51.34609345956306	23.038464453676266	13.933249356639873	11.682192730120804
11	45.27797127708338	21.977181920226954	19.496139839241177	13.24870696344848
12	41.407271322921666	21.30671584957825	24.033696550579116	13.252316276920967
13	21.95451543161975	39.55172326671744	23.727843326920787	14.765917974742024
14	15.764181894961759	42.10300258787776	27.125939774995402	15.006875742165082
15	15.089023716798827	23.834462446897973	46.56187626551554	14.514637570787661
16	16.542566438437746	18.985782914231883	46.277895481500465	18.19375516582991
17	16.81218215483233	18.26240430807656	29.233490097848485	35.69192343924262
18	21.183060770010936	24.073398998776444	34.736032859189855	20.00750737202277
19	29.55226466373831	24.107470917956693	26.40674797246816	19.933516445836837
20	32.51486134822296	22.14386001638628	25.250757053500855	20.09052158188991
21	23.151941269251175	26.791139857287742	28.43012910514291	21.62678976831817
22	22.48198050248862	24.641432753076035	25.736787205705603	27.139799538729736
23	20.285929813290213	30.458057972792997	25.12609136616124	24.12992084775555
24	20.22146747467164	23.09289290084133	39.09969284742349	17.585946777063537
25	18.728583236182644	25.354272163891707	34.884231270370066	21.032913329555587
26	18.326216970270085	33.73184966487525	27.447818350471557	20.494115014383112
27	19.325924615878815	33.65713687599482	28.00256983119241	19.01436867693396
28	16.646153735098046	29.518337117096955	36.65322798950412	17.18228115830088
29	17.270637152107298	25.02423653996773	35.75400363096936	21.951122676955617
30	19.06035133057341	31.281703307213938	30.2719617701517	19.385983592060953
31	28.070641483283467	28.096484167746453	24.47266125510267	19.360213093867415
32	29.340109217825677	26.777063534745054	26.87805212570517	17.004775121724098
33	28.87905551485052	28.46853220049014	24.265703220590414	18.386709064068924
34	20.431024214884086	28.213931228141092	27.610020897925008	23.745023659049814
35	20.753913398132543	26.550759580020284	30.396410898682962	22.298916123164215
36	30.134735671927842	27.45070580124954	23.882755061159816	18.531803465662797
37	20.681366197335606	34.06599990615785	27.915080072619386	17.33755382388716
38	20.934090326678962	33.093723042940006	23.75209791345588	22.220088716925154
39	21.726334633889287	31.714099061217567	26.533434875352356	20.026131429540786
40	25.21170428172857	26.394837238008957	26.300850715185465	22.092607765077005
41	17.502282890771344	26.430641627656005	27.790414385279778	28.276661096292877
42	22.222254305008644	26.623956457242272	24.35377046931903	26.800018768430057
43	22.53828579265938	26.274286168027977	26.26454102165227	24.92288701766037
44	19.821411169381474	30.460079188337584	28.78160405109344	20.9369055911875
45	18.031913549723708	37.17210289430847	23.17395808143333	21.62202547453449
46	21.501907522170207	34.31749686892056	25.160740775497093	19.019854833412136
47	22.114913322336957	28.394324715495866	26.19322098743598	23.297540974731195
48	22.870487004666842	25.15106781539083	30.69526205420467	21.283183125737654
49	21.423441047278395	25.411732434373658	31.929214144177635	21.23561237417031
50	19.997184735491462	33.467792291228285	26.64048711294625	19.894535860334006
51	19.19938208553351	34.03012333024136	24.554809229736414	22.215685354488723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	935.0
1	2006.0
2	3077.0
3	6363.0
4	9649.0
5	7447.0
6	5245.0
7	5440.5
8	5636.0
9	5791.0
10	5946.0
11	6483.0
12	7020.0
13	7048.5
14	7077.0
15	6536.5
16	5996.0
17	5756.5
18	5517.0
19	5436.0
20	5355.0
21	5944.5
22	6534.0
23	6726.0
24	6918.0
25	7376.5
26	10404.5
27	12974.0
28	14833.5
29	16693.0
30	19423.0
31	22153.0
32	25036.5
33	27920.0
34	33093.0
35	38266.0
36	40211.5
37	42157.0
38	49194.5
39	56232.0
40	75849.0
41	95466.0
42	116414.0
43	137362.0
44	136656.0
45	135950.0
46	131818.0
47	127686.0
48	117823.5
49	107961.0
50	100970.0
51	93979.0
52	86213.0
53	78447.0
54	69085.0
55	59723.0
56	56911.0
57	54099.0
58	52608.5
59	51118.0
60	46846.5
61	42575.0
62	37019.0
63	31463.0
64	25679.0
65	19895.0
66	17478.5
67	15062.0
68	13686.0
69	12310.0
70	10844.5
71	9379.0
72	7497.5
73	5616.0
74	4821.5
75	3018.0
76	2009.0
77	1486.0
78	963.0
79	773.0
80	583.0
81	427.5
82	272.0
83	217.0
84	162.0
85	94.5
86	27.0
87	21.0
88	15.0
89	11.0
90	7.0
91	7.5
92	8.0
93	7.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1385305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.261577536170712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5006504750618	13.090675694123949
2	6.781905612630353	2.2056896792555962
3	2.2995590923819873	1.1218337543932808
4	1.1165971110540738	0.7263052199228016
5	0.6680028348195859	0.5431389946400267
6	0.4658805976948692	0.45455720772076025
7	0.34342960222295577	0.39092949733354027
8	0.27802460356899394	0.3616894918320256
9	0.24964898024095317	0.3653717624112789
>10	3.9047482918899945	16.06897268559547
>50	1.7399674662240878	20.511709224631236
>100	1.6439969671704584	42.66687252212423
>500	0.005356492969330845	0.52292050454217
>1k	0.0017854976564436148	0.5891929116280946
>5k	4.463744141109037E-4	0.3801408498455503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5237	0.37803949310801593	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4712	0.3401417016469297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023388351301698904	0.0
2	0.0	0.0	0.0	0.07456841634152768	0.0
3	0.0	0.0	0.0	0.10712442386333695	0.0
4	0.0	0.0	0.0	0.1597482142921595	0.0
5	0.0	0.0	0.0	0.26463486380255613	0.0
6	0.0	0.0	0.0	0.3666340625349652	0.0
7	0.0	0.0	0.0	0.4258268034837094	0.0
8	0.0	0.0	0.0	0.5653628623299563	0.0
9	0.0	0.0	0.0	0.6161819960225365	0.0
10	0.0	0.0	0.0	0.7062704602957471	0.0
11	7.218626944968797E-5	0.0	0.0	0.8361335590357358	0.0
12	7.218626944968797E-5	0.0	0.0	0.9403705321210852	0.0
13	1.4437253889937595E-4	0.0	0.0	0.983682293790898	0.0
14	1.4437253889937595E-4	0.0	0.0	1.000573880842125	0.0
15	1.4437253889937595E-4	0.0	0.0	1.0282212220413556	0.0
16	1.4437253889937595E-4	0.0	0.0	1.0921060705043293	0.0
17	1.4437253889937595E-4	0.0	0.0	1.1698506827016433	0.0
18	1.4437253889937595E-4	0.0	0.0	1.2803678612291156	0.0
19	1.4437253889937595E-4	0.0	0.0	1.3333525830051867	0.0
20	1.4437253889937595E-4	0.0	0.0	1.3830889226560217	0.0
21	1.4437253889937595E-4	0.0	0.0	1.4475512612745929	0.0
22	1.4437253889937595E-4	0.0	0.0	1.5231302853884163	0.0
23	1.4437253889937595E-4	0.0	0.0	1.612280328158781	0.0
24	1.4437253889937595E-4	0.0	0.0	1.6768870393162516	0.0
25	1.4437253889937595E-4	0.0	0.0	1.7308823688646182	0.0
26	1.4437253889937595E-4	0.0	0.0	1.785094257221334	0.0
27	1.4437253889937595E-4	0.0	0.0	1.8424823414338358	0.0
28	2.1655880834906392E-4	0.0	0.0	1.90398504300497	0.0
29	2.1655880834906392E-4	0.0	0.0	1.9698189207430854	0.0
30	2.1655880834906392E-4	0.0	0.0	2.0480688368265474	0.0
31	2.1655880834906392E-4	0.0	0.0	2.1244419099043172	0.0
32	2.1655880834906392E-4	0.0	0.0	2.1965559930845555	0.0
33	2.1655880834906392E-4	0.0	0.0	2.268597889995344	0.0
34	2.1655880834906392E-4	0.0	0.0	2.3475696687733025	0.0
35	2.1655880834906392E-4	0.0	0.0	2.4510847791641552	0.0
36	2.1655880834906392E-4	0.0	0.0	2.53785267504268	0.0
37	2.1655880834906392E-4	0.0	0.0	2.628374256932589	0.0
38	2.1655880834906392E-4	0.0	0.0	2.712615633380375	0.0
39	2.1655880834906392E-4	0.0	0.0	2.8110055186402993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1658252E-6	45.000004	18
ACGTTAG	35	1.2121745E-7	45.000004	1
GACGTAT	30	2.1658252E-6	45.000004	11
TACGACT	30	2.1658252E-6	45.000004	21
TAGGCCC	30	2.1658252E-6	45.000004	35
CGACTCA	30	2.1658252E-6	45.000004	23
GGTTCGA	30	2.1658252E-6	45.000004	38
TACGTAA	35	1.2121745E-7	45.000004	18
ACACGGC	55	1.8189894E-12	45.0	31
GCGCGTT	20	7.03379E-4	45.0	25
CGCATCG	20	7.03379E-4	45.0	21
TTCGTAA	20	7.03379E-4	45.0	32
ACTTACG	40	6.8175723E-9	45.0	1
ATCCGTT	25	3.891222E-5	45.0	16
ATATCCG	20	7.03379E-4	45.0	2
CGTAGTG	25	3.891222E-5	45.0	45
ACAATCG	45	3.8562575E-10	45.0	23
CGAATAC	20	7.03379E-4	45.0	27
CGTACTA	25	3.891222E-5	45.0	26
AGCGAAT	20	7.03379E-4	45.0	25
>>END_MODULE