FastQCFastQC Report
Thu 26 May 2016
SRR1545560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545560_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2204442
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86380.391845192570274No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52420.23779260239099057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.0346973E-445.035
TAAGTCG253.8919752E-545.016
CGCCTAT207.0346973E-445.017
CGGTCTA2000.045.031
CGACTAT207.0346973E-445.036
ACGAGAT253.8919752E-545.024
TATCGGC351.2126111E-745.013
CCCGATA253.8919752E-545.038
TATACGG1950.043.8461532
TCGTAAG1250.043.1999971
ATAGCGG1850.042.567572
TACGGGA5800.041.508624
ACCCTTA603.6379788E-1241.24999629
GACGATA603.6379788E-1241.2499961
ACCCGCT5150.041.0679634
CGTAAGG3250.040.8461532
GCGTTAG2100.040.7142831
CGTTTTT65900.040.0493131
CGACGGT1800.040.028
CGGTAGT3850.039.74025712
Produced by FastQC (version 0.11.5)