##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545560_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2204442
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.324118302953764	31.0	31.0	33.0	30.0	34.0
2	31.66160915097789	31.0	31.0	34.0	30.0	34.0
3	31.76118038034115	31.0	31.0	34.0	30.0	34.0
4	35.46509411451969	37.0	35.0	37.0	33.0	37.0
5	35.333668565559904	37.0	35.0	37.0	33.0	37.0
6	35.40812822473896	37.0	35.0	37.0	33.0	37.0
7	35.851963444717526	37.0	35.0	37.0	35.0	37.0
8	35.87719114406276	37.0	35.0	37.0	35.0	37.0
9	37.55113992565919	39.0	37.0	39.0	35.0	39.0
10	36.91483377652939	39.0	37.0	39.0	32.0	39.0
11	36.55500757107694	39.0	35.0	39.0	32.0	39.0
12	35.75453198587216	37.0	35.0	39.0	31.0	39.0
13	35.39655794981224	37.0	35.0	39.0	30.0	39.0
14	36.34963995423785	38.0	35.0	40.0	31.0	41.0
15	36.58468719068136	38.0	35.0	40.0	31.0	41.0
16	36.75695890388588	38.0	35.0	41.0	32.0	41.0
17	36.7030400436936	38.0	35.0	40.0	32.0	41.0
18	36.63181975302594	38.0	35.0	40.0	31.0	41.0
19	36.6004217847419	38.0	35.0	40.0	31.0	41.0
20	36.44221485527857	38.0	35.0	40.0	31.0	41.0
21	36.250388987326495	38.0	35.0	40.0	31.0	41.0
22	36.198941047212855	37.0	35.0	40.0	30.0	41.0
23	36.16284937412733	37.0	35.0	40.0	31.0	41.0
24	36.09635998588305	37.0	35.0	40.0	31.0	41.0
25	35.9755430172352	37.0	34.0	40.0	30.0	41.0
26	35.896551145369216	37.0	34.0	40.0	30.0	41.0
27	35.830030456687	37.0	34.0	40.0	30.0	41.0
28	35.801773419305206	37.0	34.0	40.0	30.0	41.0
29	35.78985339600679	37.0	34.0	40.0	30.0	41.0
30	35.70345148568209	37.0	34.0	40.0	30.0	41.0
31	35.53875493208712	37.0	34.0	40.0	29.0	41.0
32	35.34747931676134	37.0	34.0	40.0	29.0	41.0
33	35.207792266705134	36.0	34.0	40.0	28.0	41.0
34	35.052229090173384	36.0	34.0	40.0	27.0	41.0
35	34.91905933565047	36.0	34.0	40.0	27.0	41.0
36	34.755148014781064	36.0	33.0	40.0	25.0	41.0
37	34.688438616212174	36.0	33.0	40.0	25.0	41.0
38	34.65589387246296	36.0	33.0	40.0	26.0	41.0
39	34.60659885812373	36.0	33.0	40.0	25.0	41.0
40	34.45920010596786	36.0	33.0	40.0	24.0	41.0
41	34.42029547613409	36.0	33.0	40.0	24.0	41.0
42	34.34895179823284	35.0	33.0	40.0	24.0	41.0
43	34.272278426921645	35.0	33.0	40.0	24.0	41.0
44	34.209794133844305	35.0	33.0	40.0	24.0	41.0
45	34.153508688366486	35.0	33.0	40.0	24.0	41.0
46	34.12678809422067	35.0	33.0	40.0	24.0	41.0
47	34.07054075362382	35.0	33.0	40.0	24.0	41.0
48	34.02417301067572	35.0	33.0	40.0	24.0	41.0
49	33.96336533236075	35.0	33.0	39.0	24.0	41.0
50	33.84169916922287	35.0	33.0	39.0	24.0	41.0
51	33.68605978292919	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	9.0
14	18.0
15	56.0
16	171.0
17	483.0
18	1196.0
19	2326.0
20	3951.0
21	6412.0
22	9628.0
23	13812.0
24	19471.0
25	26901.0
26	35338.0
27	42452.0
28	47340.0
29	54209.0
30	64609.0
31	78896.0
32	97066.0
33	126056.0
34	211461.0
35	319177.0
36	157725.0
37	191818.0
38	263408.0
39	430216.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.82858700750576	23.88772306098323	27.05813988301802	14.225550048492996
2	31.855272218547825	25.236046128680183	29.33962426772852	13.56905738504347
3	29.512865387249924	24.95493190567046	31.39102775214771	14.141174954931907
4	26.49427837067158	27.88456217038144	30.667806184059277	14.953353274887704
5	23.486442374079246	32.47334246035958	29.43896913595368	14.601246029607493
6	21.891027298518175	41.47194618865001	26.535377206567468	10.10164930626435
7	86.5418550363312	4.53221268692939	6.986802102300718	1.9391301744387013
8	87.37526321853785	3.4474937421805607	6.894987484361121	2.282255554920474
9	82.93481978659453	5.140575256686272	8.576592171624384	3.348012785094822
10	49.67937464446785	23.301180071873066	15.087672980282537	11.931772303376546
11	42.790375069972356	22.264364406049243	20.10390838135002	14.841352142628384
12	38.61544100502531	20.996923484491766	24.96749744379757	15.420138066685357
13	23.000423689985947	36.202585506899254	24.880219121210718	15.916771681904082
14	17.148375870174856	39.40130881193517	27.77337757128561	15.676937746604356
15	16.19371251318928	23.224289865644003	44.5603014277536	16.021696193413117
16	17.653446994749693	19.38440657545084	43.42250782737763	19.53963860242184
17	18.07727306955683	19.314955893600285	29.533686982919033	33.074084053923855
18	21.987332848856987	22.709601794921344	34.60426720231242	20.69879815390924
19	29.152683536241824	23.685177473483083	26.392756080677103	20.769382909597983
20	30.84263500695414	22.28228277269259	26.48928844578356	20.38579377456971
21	22.73368952324443	27.123598624958152	28.747546998288005	21.395164853509414
22	23.109566956173037	23.332435146853488	26.07027084405033	27.487727052923145
23	20.023116961117598	28.97694745427641	25.949696113574323	25.05023947103167
24	20.295385408189464	23.49261173575898	37.45111007683577	18.760892779215784
25	19.088231851869995	24.625642226014566	34.3414796125278	21.944646309587643
26	18.643493455486695	31.709203508189375	27.38475314841579	22.262549887908143
27	19.160132133211036	32.07442064703903	28.803343431126788	19.962103788623153
28	16.967241596739672	28.163453608668316	35.83460122788443	19.034703566707584
29	17.86783231312051	24.911111292562925	34.95628372168558	22.26477267263099
30	20.02719962693507	29.264276401919396	30.523642717748984	20.18488125339655
31	28.4889328002279	26.253945442883055	25.523737979951388	19.733383776937654
32	28.867894913996377	25.671212941869186	27.03886062776884	18.422031516365593
33	27.879799060261057	26.789137568600125	25.193268863503782	20.13779450763504
34	20.30835921289832	27.125549231959834	28.463983175787792	24.10210837935405
35	21.208450936790353	25.449478825026922	30.54573447611686	22.796335762065866
36	29.52896923575218	25.892130525547962	25.569463837107076	19.009436401592783
37	21.288289734998695	32.014178644754544	27.747339235960844	18.95019238428591
38	21.09967057423148	32.2651265036685	24.58826315230793	22.046939769792083
39	21.592629790214485	30.56233731710791	26.882358438099075	20.96267445457853
40	24.00462339222352	25.35199383789639	26.69519089184474	23.94819187803535
41	18.482409607510654	24.847693883531523	27.962949354076905	28.70694715488092
42	21.268783664981886	25.8616466207775	25.72914143352377	27.140428280716844
43	22.2149187867043	25.142689170320654	27.28831150921639	25.354080533758655
44	19.747491655484698	28.613680922428443	29.341438785869624	22.29738863621724
45	18.719657854459314	34.0232584935326	24.643742044472024	22.613341607536057
46	21.097901419043914	31.864934527649176	26.675004377525013	20.3621596757819
47	21.628466523501185	27.438372159485258	27.65711232139471	23.276048995618844
48	22.729380042659322	24.527023165045847	30.687992698379002	22.05560409391583
49	21.317322025256278	24.584089760583407	32.292298912831455	21.806289301328864
50	20.000934476842666	31.09467157675276	28.044648033379875	20.859745913024703
51	19.66973955313862	31.27889053102781	26.027176038199233	23.024193877634342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1671.0
1	3360.0
2	5049.0
3	10845.0
4	16641.0
5	12572.5
6	8504.0
7	8828.0
8	9152.0
9	9250.5
10	9349.0
11	9508.0
12	9667.0
13	9738.5
14	9810.0
15	9653.5
16	9497.0
17	9026.0
18	8555.0
19	8679.5
20	8804.0
21	8405.5
22	8007.0
23	7960.0
24	7913.0
25	10199.0
26	15608.0
27	18731.0
28	21992.0
29	25253.0
30	28500.0
31	31747.0
32	38654.5
33	45562.0
34	51633.0
35	57704.0
36	61387.5
37	65071.0
38	74390.5
39	83710.0
40	113321.5
41	142933.0
42	168327.5
43	193722.0
44	201555.0
45	209388.0
46	202635.5
47	195883.0
48	185193.5
49	174504.0
50	166065.0
51	157626.0
52	143095.5
53	128565.0
54	121808.5
55	115052.0
56	102109.5
57	89167.0
58	85042.0
59	80917.0
60	77371.5
61	73826.0
62	65092.5
63	56359.0
64	47896.5
65	39434.0
66	34538.5
67	29643.0
68	26680.0
69	23717.0
70	20035.5
71	16354.0
72	13652.0
73	10950.0
74	8736.5
75	4966.5
76	3410.0
77	2702.0
78	1994.0
79	1474.0
80	954.0
81	637.5
82	321.0
83	244.0
84	167.0
85	126.5
86	86.0
87	63.0
88	40.0
89	27.0
90	14.0
91	9.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2204442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.98175050791581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.28138972367861	13.633185307162663
2	7.764453038867498	2.637080086729532
3	2.662071312545177	1.3561989259176652
4	1.337957095441594	0.9088341434033942
5	0.78249423145927	0.6644060906262327
6	0.48928695402668837	0.49853693880355776
7	0.3707346358890785	0.440700616391794
8	0.28213546359918834	0.38329232418212633
9	0.23741695934488294	0.3628580012948514
>10	2.8222564037443227	11.964891526049072
>50	1.3646451866927785	16.982266328846222
>100	1.5882286386651234	47.673374772699674
>500	0.015586676994549544	1.7102564157577451
>1k	8.062074307525626E-4	0.1506902200029906
>5k	5.374716205017084E-4	0.6334283021324983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8638	0.391845192570274	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5242	0.23779260239099057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.536295352746863E-5	0.0	0.0	0.04372988720047976	0.0
2	4.536295352746863E-5	0.0	0.0	0.13382071290603245	0.0
3	9.072590705493726E-5	0.0	0.0	0.1910233973041704	0.0
4	9.072590705493726E-5	0.0	0.0	0.2949952867891285	0.0
5	9.072590705493726E-5	0.0	0.0	0.4871981208850131	0.0
6	9.072590705493726E-5	0.0	0.0	0.6771328073045242	0.0
7	9.072590705493726E-5	0.0	0.0	0.7884534952609322	0.0
8	9.072590705493726E-5	0.0	0.0	1.0565939135617992	0.0
9	9.072590705493726E-5	0.0	0.0	1.1556666040657908	0.0
10	9.072590705493726E-5	0.0	0.0	1.3489127860928072	0.0
11	9.072590705493726E-5	0.0	0.0	1.5888828102531163	0.0
12	9.072590705493726E-5	0.0	0.0	1.8029959509027682	0.0
13	9.072590705493726E-5	0.0	0.0	1.8819728529940911	0.0
14	9.072590705493726E-5	0.0	0.0	1.9110505062051983	0.0
15	9.072590705493726E-5	0.0	0.0	1.960178584875447	0.0
16	9.072590705493726E-5	0.0	0.0	2.0763984718128214	0.0
17	9.072590705493726E-5	0.0	0.0	2.2123966064881726	0.0
18	9.072590705493726E-5	0.0	0.0	2.393939146505102	0.0
19	9.072590705493726E-5	0.0	0.0	2.480128758207292	0.0
20	9.072590705493726E-5	0.0	0.0	2.5736218054274054	0.0
21	9.072590705493726E-5	0.0	0.0	2.6902045959929994	0.0
22	9.072590705493726E-5	0.0	0.0	2.809781341491407	0.0
23	9.072590705493726E-5	0.0	0.0	2.95775529589801	0.0
24	9.072590705493726E-5	0.0	0.0	3.0683048136444504	0.0
25	9.072590705493726E-5	0.0	0.0	3.1568532989300695	0.0
26	9.072590705493726E-5	0.0	0.0	3.243178999492842	0.0
27	9.072590705493726E-5	0.0	0.0	3.3312284922896587	0.0
28	9.072590705493726E-5	0.0	0.0	3.422045125251651	0.0
29	9.072590705493726E-5	0.0	0.0	3.52492830385195	0.0
30	9.072590705493726E-5	0.0	0.0	3.652307477357082	0.0
31	9.072590705493726E-5	0.0	0.0	3.7748328148347747	0.0
32	9.072590705493726E-5	0.0	0.0	3.8850647919065233	0.0
33	9.072590705493726E-5	0.0	0.0	4.000014516145129	0.0
34	9.072590705493726E-5	0.0	0.0	4.111380567055065	0.0
35	9.072590705493726E-5	0.0	0.0	4.256179114714744	0.0
36	9.072590705493726E-5	0.0	0.0	4.382651029149327	0.0
37	9.072590705493726E-5	0.0	0.0	4.511935446702612	0.0
38	9.072590705493726E-5	0.0	0.0	4.641038412441788	0.0
39	9.072590705493726E-5	0.0	0.0	4.774586947626656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.0346973E-4	45.0	35
TAAGTCG	25	3.8919752E-5	45.0	16
CGCCTAT	20	7.0346973E-4	45.0	17
CGGTCTA	200	0.0	45.0	31
CGACTAT	20	7.0346973E-4	45.0	36
ACGAGAT	25	3.8919752E-5	45.0	24
TATCGGC	35	1.2126111E-7	45.0	13
CCCGATA	25	3.8919752E-5	45.0	38
TATACGG	195	0.0	43.846153	2
TCGTAAG	125	0.0	43.199997	1
ATAGCGG	185	0.0	42.56757	2
TACGGGA	580	0.0	41.50862	4
ACCCTTA	60	3.6379788E-12	41.249996	29
GACGATA	60	3.6379788E-12	41.249996	1
ACCCGCT	515	0.0	41.06796	34
CGTAAGG	325	0.0	40.846153	2
GCGTTAG	210	0.0	40.714283	1
CGTTTTT	6590	0.0	40.049313	1
CGACGGT	180	0.0	40.0	28
CGGTAGT	385	0.0	39.740257	12
>>END_MODULE