FastQCFastQC Report
Thu 26 May 2016
SRR1545559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545559_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1919928
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70380.3665762466092478No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35050.18255892929318182No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21490.1119312807563617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGT351.212502E-745.00000420
CGAATCG351.212502E-745.00000419
GCGATCG207.034468E-445.0000049
TTACGTC207.034468E-445.00000420
CCGAAAC207.034468E-445.00000433
ATACGTC302.1662654E-645.00000414
TGTACGA207.034468E-445.00000436
GCCCGTA302.1662654E-645.00000418
TCGCCTA253.8917868E-545.044
CTATCGA253.8917868E-545.038
ACTTACG253.8917868E-545.01
TATAGCG453.8562575E-1045.01
GCGTATT253.8917868E-545.041
GTACGAC253.8917868E-545.035
ATAGCGG2500.043.1999972
CGGTCTA3400.043.01470631
CGACGGT3450.042.39130428
TAATGCG700.041.7857171
CGTATGG1950.041.538462
CATACGA4250.041.29411718
Produced by FastQC (version 0.11.5)