##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545559_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1919928 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.273437337233478 31.0 31.0 33.0 30.0 34.0 2 31.617814834722967 31.0 31.0 34.0 30.0 34.0 3 31.728456483784807 31.0 31.0 34.0 30.0 34.0 4 35.44237961006871 37.0 35.0 37.0 33.0 37.0 5 35.2959340141922 37.0 35.0 37.0 33.0 37.0 6 35.37715216403949 37.0 35.0 37.0 33.0 37.0 7 35.81780306344821 37.0 35.0 37.0 35.0 37.0 8 35.83592561804401 37.0 35.0 37.0 35.0 37.0 9 37.52417069806784 39.0 37.0 39.0 35.0 39.0 10 36.87432132871649 39.0 37.0 39.0 32.0 39.0 11 36.488191744690425 39.0 35.0 39.0 32.0 39.0 12 35.69411769608027 37.0 35.0 39.0 31.0 39.0 13 35.36131771608102 37.0 35.0 39.0 30.0 39.0 14 36.35021313299249 38.0 35.0 40.0 31.0 41.0 15 36.59333110408307 38.0 35.0 41.0 31.0 41.0 16 36.74546441324883 38.0 35.0 41.0 32.0 41.0 17 36.660536749294764 38.0 35.0 40.0 31.0 41.0 18 36.616924697176145 38.0 35.0 40.0 31.0 41.0 19 36.55432182873525 38.0 35.0 40.0 31.0 41.0 20 36.420104816430616 38.0 35.0 40.0 31.0 41.0 21 36.23399627486031 38.0 35.0 40.0 31.0 41.0 22 36.15994401873404 38.0 35.0 40.0 30.0 41.0 23 36.12006700251259 37.0 34.0 40.0 30.0 41.0 24 36.050799300807114 37.0 34.0 40.0 30.0 41.0 25 35.936699709572444 37.0 34.0 40.0 30.0 41.0 26 35.82815136817631 37.0 34.0 40.0 30.0 41.0 27 35.76760013917188 37.0 34.0 40.0 30.0 41.0 28 35.75477934589214 37.0 34.0 40.0 30.0 41.0 29 35.73333635427995 37.0 34.0 40.0 30.0 41.0 30 35.64223293790184 37.0 34.0 40.0 29.0 41.0 31 35.47838096011934 37.0 34.0 40.0 29.0 41.0 32 35.32951027330191 37.0 34.0 40.0 29.0 41.0 33 35.15608762411924 37.0 34.0 40.0 27.0 41.0 34 35.000634398789956 36.0 34.0 40.0 27.0 41.0 35 34.87322337087641 36.0 34.0 40.0 26.0 41.0 36 34.69301557141726 36.0 33.0 40.0 25.0 41.0 37 34.635945722964614 36.0 33.0 40.0 25.0 41.0 38 34.620406077727914 36.0 33.0 40.0 26.0 41.0 39 34.55915534332537 36.0 33.0 40.0 25.0 41.0 40 34.39501377134976 36.0 33.0 40.0 24.0 41.0 41 34.32648932668308 36.0 33.0 40.0 24.0 41.0 42 34.246895716922715 35.0 33.0 40.0 24.0 41.0 43 34.164531169918874 35.0 33.0 40.0 24.0 41.0 44 34.12817511906697 35.0 33.0 40.0 24.0 41.0 45 34.06893018904876 35.0 33.0 40.0 23.0 41.0 46 34.02073931939115 35.0 33.0 40.0 23.0 41.0 47 33.96656853798684 35.0 33.0 40.0 23.0 41.0 48 33.9163447795959 35.0 33.0 39.0 23.0 41.0 49 33.84242117412737 35.0 33.0 39.0 24.0 41.0 50 33.7173696096937 35.0 33.0 39.0 24.0 41.0 51 33.5323631927864 35.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 7.0 14 19.0 15 63.0 16 172.0 17 533.0 18 1141.0 19 2273.0 20 3756.0 21 6047.0 22 8836.0 23 12630.0 24 17664.0 25 24082.0 26 31400.0 27 37407.0 28 42534.0 29 48696.0 30 57208.0 31 69821.0 32 85435.0 33 109585.0 34 182420.0 35 270484.0 36 139238.0 37 170304.0 38 234896.0 39 363082.0 40 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.931309924122154 23.179515065148276 27.957454654549547 13.931720356180024 2 31.04741427803543 24.44294786054477 30.884439416478116 13.625198444941686 3 29.94034151280673 25.258603447629284 31.075748673908603 13.72530636565538 4 26.86595538999379 27.88656657958007 30.281395969015506 14.966082061410635 5 23.159097632827898 32.936495535249236 29.633402919276143 14.271003912646723 6 20.94870224300078 42.723164618673195 26.103791392177207 10.224341746148815 7 87.25207403610969 4.44334370872241 6.478784621089957 1.8257976340779445 8 87.77719789492106 3.618521111208337 6.591028413565509 2.0132525803050947 9 84.112945902138 4.988416232275377 7.969465521623728 2.9291723439628985 10 50.383712305878134 24.056735460913117 13.469046755920013 12.090505477288731 11 43.51303798892459 21.338196015683923 20.480924284660677 14.66784171073082 12 38.537955590001296 21.004068902583846 25.162245667545868 15.295729839868995 13 22.926745169610527 35.767278772953986 25.203497214478876 16.10247884295661 14 16.928707743207035 38.06564621173294 28.321478722118748 16.684167322941278 15 16.754326203899314 22.906223566717085 43.832841648228474 16.506608581155128 16 18.09307432362047 18.627208937001804 43.30740527769791 19.972311461679812 17 18.020363263622386 18.41027371859778 30.471142667850042 33.09822034992979 18 21.93514548462234 22.160310178298353 34.081538474359455 21.823005862719853 19 29.030359471813526 23.790058793871438 25.751538599364142 21.428043134950894 20 30.504789762949443 22.938516444366662 25.825812217958173 20.730881574725718 21 24.4905017271481 26.249994791471348 28.339031463679888 20.920472017700664 22 23.53275747840544 23.42353463254872 25.571323507964884 27.472384381080957 23 20.70343262872358 28.481901404636005 25.516633957106727 25.29803200953369 24 20.745048772662308 23.303269705947308 37.06482743102866 18.88685409036172 25 20.027886462409008 24.177052472801066 34.04080778029176 21.75425328449817 26 18.957950506477324 31.239452729477357 26.62261293131826 23.17998383272706 27 19.336714710134963 31.463731973282332 27.88922292919318 21.310330387389527 28 17.14434082944777 27.72046660083086 35.92681600560021 19.208376564121153 29 18.402408840331514 24.3392460550604 35.012562971111414 22.24578213349667 30 20.644784596089018 28.890197965757046 29.923570050543564 20.54144738761037 31 28.864311578350854 26.4433874603631 24.726656416282278 19.965644545003773 32 29.989562108579072 24.867130434057945 26.162647765957892 18.980659691405094 33 27.96198607447779 25.92279502147997 25.17557950089795 20.939639403144284 34 20.854792471384343 27.103360126004727 27.634109195761507 24.407738206849423 35 21.713887187436196 24.513002570929743 30.334106278985463 23.4390039626486 36 29.70522852940319 25.849198511610854 24.993176827464364 19.4523961315216 37 22.160466434157946 31.278777120808698 27.235500497935337 19.325255947098015 38 21.987803709305766 31.772024784262744 24.313411752940738 21.926759753490757 39 22.413965523707137 29.961279797992425 25.461371468096722 22.163383210203715 40 24.736500535436747 25.33266872507719 26.4442208249476 23.486609914538462 41 19.094934810055378 23.974596964052818 27.801302965527874 29.129165260363933 42 22.089005421036624 25.523248788496232 25.26495785258614 27.122787937881004 43 21.81529724031318 25.34746094645216 26.693136409282015 26.14410540395265 44 20.664629090257552 28.097199478313772 28.532372047285104 22.70579938414357 45 19.184417332316627 33.75798467442529 24.04100570438058 23.0165922888775 46 21.239390227133516 31.47524282160581 26.123740056918805 21.16162689434187 47 22.232239958998463 27.618223183369377 26.244109154093277 23.905427703538884 48 23.125658878874624 24.696290694234367 30.007896129438187 22.17015429745282 49 21.48137846835923 24.81874320287011 31.312320045335035 22.38755828343563 50 20.77807084432333 31.197471988532904 26.78772328962336 21.236733877520404 51 19.102643432462052 32.98670575146568 25.042449508523234 22.868201307549032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1387.0 1 2790.5 2 4194.0 3 8935.5 4 13677.0 5 10617.0 6 7557.0 7 7647.0 8 7737.0 9 7942.0 10 8147.0 11 8276.0 12 8405.0 13 8115.5 14 7826.0 15 7605.5 16 7385.0 17 6959.5 18 6534.0 19 6721.5 20 6909.0 21 6934.5 22 6960.0 23 7290.0 24 7620.0 25 9176.0 26 13124.5 27 15517.0 28 17264.0 29 19011.0 30 23974.0 31 28937.0 32 33964.0 33 38991.0 34 43980.0 35 48969.0 36 48929.5 37 48890.0 38 60724.0 39 72558.0 40 94355.0 41 116152.0 42 144929.0 43 173706.0 44 171383.5 45 169061.0 46 166341.5 47 163622.0 48 153512.5 49 143403.0 50 138318.5 51 133234.0 52 119904.5 53 106575.0 54 102334.0 55 98093.0 56 93877.5 57 89662.0 58 86308.0 59 82954.0 60 77620.5 61 72287.0 62 64029.5 63 55772.0 64 48741.0 65 41710.0 66 36035.5 67 30361.0 68 26838.0 69 23315.0 70 19170.0 71 15025.0 72 13015.5 73 11006.0 74 8869.5 75 5614.5 76 4496.0 77 3462.0 78 2428.0 79 1896.0 80 1364.0 81 978.0 82 592.0 83 422.0 84 252.0 85 176.5 86 101.0 87 75.5 88 50.0 89 33.0 90 16.0 91 12.5 92 9.0 93 7.0 94 5.0 95 2.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1919928.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.836973973771865 #Duplication Level Percentage of deduplicated Percentage of total 1 80.59830789463122 13.570316123519572 2 7.139256215698015 2.40406942191593 3 2.5580506409528656 1.292094961959415 4 1.2457224795059973 0.8389678786394014 5 0.743841706174202 0.6262021723730549 6 0.49779574495464346 0.5028824401233425 7 0.3568085750718834 0.4205303684472748 8 0.297309976343974 0.40046402670769754 9 0.2384818077629982 0.36137807914712994 >10 3.217606518229546 13.614798408555565 >50 1.5123254695215331 18.573828469471692 >100 1.5832882577115948 45.57134149008242 >500 0.009959745281453113 1.1015266416610847 >1k 9.337261201362293E-4 0.35278198628270324 >5k 3.112420400454098E-4 0.3688175311137353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7038 0.3665762466092478 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 3505 0.18255892929318182 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2149 0.1119312807563617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.208528653157827E-5 0.0 0.0 0.021094541045289197 0.0 2 5.208528653157827E-5 0.0 0.0 0.07026305153109909 0.0 3 1.0417057306315654E-4 0.0 0.0 0.10036834714635132 0.0 4 1.0417057306315654E-4 0.0 0.0 0.17266272485218195 0.0 5 1.0417057306315654E-4 0.0 0.0 0.29777158310103297 0.0 6 1.0417057306315654E-4 0.0 0.0 0.44126654749553107 0.0 7 1.0417057306315654E-4 0.0 0.0 0.5302282168914667 0.0 8 1.0417057306315654E-4 0.0 0.0 0.768310061627311 0.0 9 1.0417057306315654E-4 0.0 0.0 0.8520632023700889 0.0 10 1.0417057306315654E-4 0.0 0.0 1.0008708659908079 0.0 11 1.0417057306315654E-4 0.0 0.0 1.1398344104570588 0.0 12 1.0417057306315654E-4 0.0 0.0 1.272703976399115 0.0 13 1.0417057306315654E-4 0.0 0.0 1.3205182694351039 0.0 14 1.562558595947348E-4 0.0 0.0 1.3373418169848037 0.0 15 2.0834114612631308E-4 0.0 0.0 1.3674991978865874 0.0 16 2.0834114612631308E-4 0.0 0.0 1.433751682354755 0.0 17 2.0834114612631308E-4 0.0 0.0 1.5140671941864488 0.0 18 2.0834114612631308E-4 0.0 0.0 1.6359988499568734 0.0 19 2.0834114612631308E-4 0.0 0.0 1.691261338966878 0.0 20 2.0834114612631308E-4 0.0 0.0 1.7519406977761667 0.0 21 2.0834114612631308E-4 0.0 0.0 1.835641753232413 0.0 22 2.0834114612631308E-4 0.0 0.0 1.9255930430724486 0.0 23 2.0834114612631308E-4 0.0 0.0 2.029659445562542 0.0 24 4.1668229225262616E-4 0.0 0.0 2.108933251663604 0.0 25 4.1668229225262616E-4 0.0 0.0 2.174248200974203 0.0 26 4.1668229225262616E-4 0.0 0.0 2.2343546216316446 0.0 27 4.1668229225262616E-4 0.0 0.0 2.2969611360426017 0.0 28 4.1668229225262616E-4 0.0 0.0 2.365192861398969 0.0 29 4.1668229225262616E-4 0.0 0.0 2.444622923359626 0.0 30 4.1668229225262616E-4 0.0 0.0 2.5396785712797563 0.0 31 4.1668229225262616E-4 0.0 0.0 2.632598722452092 0.0 32 4.1668229225262616E-4 0.0 0.0 2.7197374068194224 0.0 33 4.1668229225262616E-4 0.0 0.0 2.815730589897121 0.0 34 4.1668229225262616E-4 0.0 0.0 2.907713205911888 0.0 35 4.1668229225262616E-4 0.0 0.0 3.0311032497051973 0.0 36 4.1668229225262616E-4 0.0 0.0 3.136523869645112 0.0 37 4.1668229225262616E-4 0.0 0.0 3.244079986332821 0.0 38 4.1668229225262616E-4 0.0 0.0 3.348406815255572 0.0 39 4.1668229225262616E-4 0.0 0.0 3.460181840152339 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATCGT 35 1.212502E-7 45.000004 20 CGAATCG 35 1.212502E-7 45.000004 19 GCGATCG 20 7.034468E-4 45.000004 9 TTACGTC 20 7.034468E-4 45.000004 20 CCGAAAC 20 7.034468E-4 45.000004 33 ATACGTC 30 2.1662654E-6 45.000004 14 TGTACGA 20 7.034468E-4 45.000004 36 GCCCGTA 30 2.1662654E-6 45.000004 18 TCGCCTA 25 3.8917868E-5 45.0 44 CTATCGA 25 3.8917868E-5 45.0 38 ACTTACG 25 3.8917868E-5 45.0 1 TATAGCG 45 3.8562575E-10 45.0 1 GCGTATT 25 3.8917868E-5 45.0 41 GTACGAC 25 3.8917868E-5 45.0 35 ATAGCGG 250 0.0 43.199997 2 CGGTCTA 340 0.0 43.014706 31 CGACGGT 345 0.0 42.391304 28 TAATGCG 70 0.0 41.785717 1 CGTATGG 195 0.0 41.53846 2 CATACGA 425 0.0 41.294117 18 >>END_MODULE