FastQCFastQC Report
Thu 26 May 2016
SRR1545558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545558_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2617604
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111570.42622948314565534No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC69250.2645549135774548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA406.8212103E-945.00000423
CGACATA207.0349395E-445.00000443
ATCGTAC406.8212103E-945.00000428
AACGCAT351.2127202E-745.00000438
TCGTAAT351.2127202E-745.00000417
ACGCGGT750.045.00000423
TACGCGC1100.045.034
ACGTAAG1000.045.01
TTCGATC253.8921768E-545.017
TCGAACA253.8921768E-545.025
TATATCG302.1665692E-644.99999627
CGTCGGA302.1665692E-644.99999619
CGACGGT2500.044.10000228
CGGTCTA2500.043.231
TACGGGA8200.042.804884
TCTAGCG800.042.1875041
CGTTATC1350.041.66666434
CGTTTTT80050.041.374141
AACCGAG603.6379788E-1241.2499961
TACGCCT556.184564E-1140.90909235