##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545558_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2617604
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36998300736093	31.0	31.0	33.0	30.0	34.0
2	31.709446883485814	31.0	31.0	34.0	30.0	34.0
3	31.80996781789759	31.0	31.0	34.0	30.0	34.0
4	35.504931227183334	37.0	35.0	37.0	33.0	37.0
5	35.367387121963446	37.0	35.0	37.0	33.0	37.0
6	35.44162065767014	37.0	35.0	37.0	33.0	37.0
7	35.91950501298134	37.0	35.0	37.0	35.0	37.0
8	35.95648539656877	37.0	35.0	37.0	35.0	37.0
9	37.68312968653777	39.0	37.0	39.0	35.0	39.0
10	37.0103934743376	39.0	37.0	39.0	32.0	39.0
11	36.532375408961784	39.0	35.0	39.0	32.0	39.0
12	35.7775671950379	37.0	35.0	39.0	31.0	39.0
13	35.43163595410154	37.0	35.0	39.0	30.0	39.0
14	36.43217843493515	38.0	35.0	41.0	31.0	41.0
15	36.69292184761331	38.0	35.0	41.0	31.0	41.0
16	36.87077380688599	38.0	35.0	41.0	32.0	41.0
17	36.811501663353205	38.0	35.0	41.0	32.0	41.0
18	36.738061983401614	38.0	35.0	40.0	31.0	41.0
19	36.69763073406062	38.0	35.0	40.0	31.0	41.0
20	36.55612575469781	38.0	35.0	40.0	31.0	41.0
21	36.370484229088895	38.0	35.0	40.0	31.0	41.0
22	36.292666499592755	38.0	35.0	40.0	31.0	41.0
23	36.25253514282527	38.0	35.0	40.0	31.0	41.0
24	36.192598269256926	37.0	35.0	40.0	31.0	41.0
25	36.1047939260484	37.0	35.0	40.0	30.0	41.0
26	36.02590002154642	37.0	35.0	40.0	30.0	41.0
27	35.96801540645568	37.0	34.0	40.0	30.0	41.0
28	35.97845625235903	37.0	35.0	40.0	30.0	41.0
29	35.99300314333261	37.0	35.0	40.0	30.0	41.0
30	35.91967539780654	37.0	35.0	40.0	30.0	41.0
31	35.735431715416084	37.0	34.0	40.0	30.0	41.0
32	35.55152383630221	37.0	34.0	40.0	29.0	41.0
33	35.37988519271823	37.0	34.0	40.0	29.0	41.0
34	35.235840868213835	37.0	34.0	40.0	28.0	41.0
35	35.09516641936672	37.0	34.0	40.0	27.0	41.0
36	34.93045242901524	37.0	34.0	40.0	26.0	41.0
37	34.87544907480276	37.0	34.0	40.0	26.0	41.0
38	34.857324866557356	36.0	34.0	40.0	27.0	41.0
39	34.82365552619877	36.0	34.0	40.0	26.0	41.0
40	34.66421124050849	36.0	34.0	40.0	25.0	41.0
41	34.64869208635072	36.0	34.0	40.0	25.0	41.0
42	34.573739572525106	36.0	33.0	40.0	25.0	41.0
43	34.47064338226867	36.0	33.0	40.0	25.0	41.0
44	34.41835205019552	36.0	33.0	40.0	24.0	41.0
45	34.37966323401095	35.0	33.0	40.0	24.0	41.0
46	34.36333035860275	35.0	33.0	40.0	24.0	41.0
47	34.30833884728171	35.0	33.0	40.0	24.0	41.0
48	34.29318147435594	35.0	33.0	40.0	24.0	41.0
49	34.23421418977049	36.0	33.0	40.0	24.0	41.0
50	34.11399929095463	35.0	33.0	40.0	24.0	41.0
51	33.96081263628876	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	7.0
14	12.0
15	67.0
16	245.0
17	585.0
18	1281.0
19	2621.0
20	4557.0
21	7114.0
22	11026.0
23	15889.0
24	22465.0
25	30933.0
26	40625.0
27	48079.0
28	53466.0
29	61178.0
30	72628.0
31	88603.0
32	110422.0
33	143504.0
34	241148.0
35	382915.0
36	179851.0
37	220636.0
38	320868.0
39	556614.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.03180924234529	24.22486365393696	26.123775788851177	15.619551314866573
2	29.224053752974093	27.33052822352044	30.008702615063243	13.436715408442224
3	27.81398561432516	26.535564585017447	32.26229024711148	13.388159553545915
4	24.90193321831721	29.001254582434928	31.4685491006279	14.628263098619959
5	22.89425749655028	33.50678712288032	30.18290772783049	13.416047652738916
6	20.616105415486835	42.37352173972839	26.567081957393096	10.443290887391676
7	88.92334363792231	3.6273630388706617	6.132287389536385	1.3170059336706392
8	90.51338552355513	2.5839278974206947	5.679697922222002	1.2229886568021748
9	86.60549876910335	4.428400934595149	6.84656655475771	2.1195337415437936
10	51.48857504802101	27.78533345762002	11.824974289464716	8.901117204894247
11	40.28382444403355	20.708365360077384	24.863386516829895	14.14442367905917
12	38.07264200390892	20.624204425115487	26.938146488162456	14.365007082813138
13	21.01601311733937	37.60152414192521	26.6072331796559	14.775229561079522
14	15.230569635437599	40.635405508243416	29.072082713810033	15.061942142508952
15	14.72594785154668	24.444950420308036	46.14414556212475	14.68495616602053
16	16.880819253026814	20.245270101971116	44.32377853945822	18.55013210554385
17	17.059608710866883	19.465625816586467	30.39359658680228	33.08116888574437
18	20.910573180664453	22.723987279970537	35.68316674332711	20.682272796037903
19	26.95071523423711	24.150941089637698	27.91140294712264	20.986940729002555
20	30.095652359944435	23.479258130718016	27.355245484038075	19.069844025299474
21	22.340086582997277	27.70781982301372	30.15742640980072	19.794667184188288
22	22.708324100971726	22.745533701812803	28.183292812816607	26.362849384398864
23	19.01555773906213	29.14665472699461	26.923973221312313	24.91381431263094
24	19.48354296524608	23.99132947535227	39.76888788372878	16.75623967567287
25	17.39174451139286	25.731967096627294	36.08555763209409	20.790730759885758
26	17.48549436813208	33.201698958283984	28.972029382595686	20.340777290988246
27	17.817515560031236	33.87594150986933	29.927597910149895	18.37894501994954
28	15.304033765229578	28.81799538814886	37.28123123283736	18.59673961378421
29	16.180751557531238	23.649299130044117	38.040207762518705	22.129741549905944
30	18.7756436802511	29.831288460745014	32.36207615819658	19.030991700807302
31	27.718134599427568	27.643256963238137	26.176075525556957	18.462532911777334
32	28.127325600052565	26.000456906392255	28.179090496499853	17.693126997055323
33	27.05550572202671	27.735516907828682	26.033349582289755	19.17562778785485
34	19.533397717913022	28.150705759923962	28.711906002588627	23.60399051957439
35	19.55299579309934	26.19055441541196	32.547436510641035	21.709013280847675
36	28.938525460688474	25.094704928629387	28.045915272134366	17.92085433854777
37	20.875006303474475	31.475769444117596	29.888363556901652	17.760860695506274
38	20.94862324476888	31.7015484389541	25.459122159043158	21.890706157233865
39	20.586994824274413	31.346643724566437	28.17683652683905	19.889524924320103
40	24.032856001136917	26.773874123052995	26.68566368327677	22.507606192533324
41	17.705581134503156	25.476619076071096	29.38202264360843	27.43577714581732
42	22.674629164686486	24.686774622899417	26.172904686881594	26.46569152553251
43	22.174668131619605	26.16759448717224	27.254237080933557	24.4035003002746
44	18.998557459417086	29.969162638810147	30.010956584724045	21.021323317048722
45	17.990039746271783	36.48550353682222	24.68925780981386	20.835198907092135
46	21.795772011350838	32.35982218853577	27.330337209142407	18.514068590970982
47	21.308838158865896	29.320439608130183	27.060472095855598	22.310250137148323
48	22.72555359787042	25.34458993797381	31.054391726174014	20.87546473798176
49	21.486405124686545	24.479218399727383	32.254038425980404	21.780338049605668
50	19.052958354281245	33.34622043670471	28.16174639097434	19.439074818039703
51	18.81220383220686	33.29124649870645	25.69590358205443	22.200646087032265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2438.0
1	3705.5
2	4973.0
3	12464.5
4	19956.0
5	14837.0
6	9718.0
7	9900.5
8	10083.0
9	10664.0
10	11245.0
11	11460.0
12	11675.0
13	11611.0
14	11547.0
15	11253.0
16	10959.0
17	10380.5
18	9802.0
19	10416.0
20	11030.0
21	11761.5
22	12493.0
23	13059.0
24	13625.0
25	15827.5
26	23282.0
27	28534.0
28	31010.0
29	33486.0
30	43997.0
31	54508.0
32	57017.5
33	59527.0
34	74583.5
35	89640.0
36	90264.0
37	90888.0
38	107289.5
39	123691.0
40	162126.5
41	200562.0
42	225404.5
43	250247.0
44	253179.5
45	256112.0
46	243855.5
47	231599.0
48	216086.5
49	200574.0
50	190502.0
51	180430.0
52	169359.5
53	158289.0
54	139694.5
55	121100.0
56	109416.5
57	97733.0
58	88469.5
59	79206.0
60	72381.5
61	65557.0
62	55584.0
63	45611.0
64	40062.5
65	34514.0
66	26503.5
67	18493.0
68	16722.5
69	14952.0
70	12177.5
71	9403.0
72	8270.0
73	7137.0
74	5267.0
75	2924.5
76	2452.0
77	1695.5
78	939.0
79	991.5
80	1044.0
81	665.0
82	286.0
83	169.0
84	52.0
85	41.5
86	31.0
87	23.0
88	15.0
89	8.0
90	1.0
91	5.0
92	9.0
93	7.5
94	6.0
95	4.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2617604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.436510910952846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57465075098483	11.632168247128075
2	7.655785408277459	2.2104565915702223
3	2.614744556152634	1.1324336494275618
4	1.3344844647676477	0.7706119814446086
5	0.8060920221302951	0.5818578136358025
6	0.5189045243792696	0.44947024967664734
7	0.36024291526243946	0.36404555437457686
8	0.27488819407483644	0.31747411304427997
9	0.21483917652291018	0.2791375304375806
>10	2.4392994561943415	8.553078917885017
>50	1.1505988379453909	12.123329404811575
>100	2.033107375702564	58.70441331018939
>500	0.02049879113821298	1.9130979752648973
>1k	0.0013310903336501937	0.27348638338191594
>5k	2.662180667300387E-4	0.26614575673408764
>10k+	2.662180667300387E-4	0.42879252099382176
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11157	0.42622948314565534	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6925	0.2645549135774548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.820287560685268E-5	0.0	0.0	0.02166103046908547	0.0
2	3.820287560685268E-5	0.0	0.0	0.07166859463845562	0.0
3	3.820287560685268E-5	0.0	0.0	0.10112301173133904	0.0
4	3.820287560685268E-5	0.0	0.0	0.1667937548995188	0.0
5	3.820287560685268E-5	0.0	0.0	0.27444945835962964	0.0
6	3.820287560685268E-5	0.0	0.0	0.3763747304787126	0.0
7	3.820287560685268E-5	0.0	0.0	0.4381487803349934	0.0
8	3.820287560685268E-5	0.0	0.0	0.5748386692563122	0.0
9	3.820287560685268E-5	0.0	0.0	0.6282080864790854	0.0
10	3.820287560685268E-5	0.0	0.0	0.7433897564337463	0.0
11	3.820287560685268E-5	0.0	0.0	0.8745020255164647	0.0
12	3.820287560685268E-5	0.0	0.0	1.003322122062772	0.0
13	3.820287560685268E-5	0.0	0.0	1.0475992548911142	0.0
14	3.820287560685268E-5	0.0	0.0	1.064714143162984	0.0
15	3.820287560685268E-5	0.0	0.0	1.094818009141184	0.0
16	3.820287560685268E-5	0.0	0.0	1.1559044072365414	0.0
17	3.820287560685268E-5	0.0	0.0	1.234373113733017	0.0
18	3.820287560685268E-5	0.0	0.0	1.3474918284049078	0.0
19	3.820287560685268E-5	0.0	0.0	1.4150345124778232	0.0
20	3.820287560685268E-5	0.0	0.0	1.4734085064050941	0.0
21	3.820287560685268E-5	0.0	0.0	1.5497760547431927	0.0
22	3.820287560685268E-5	0.0	0.0	1.6348538587196535	0.0
23	3.820287560685268E-5	0.0	0.0	1.7344487554267185	0.0
24	3.820287560685268E-5	0.0	0.0	1.8103578692575348	0.0
25	3.820287560685268E-5	0.0	0.0	1.8711768472236443	0.0
26	3.820287560685268E-5	0.0	0.0	1.9304677101654795	0.0
27	3.820287560685268E-5	0.0	0.0	1.9932350347875385	0.0
28	3.820287560685268E-5	0.0	0.0	2.0594024153386075	0.0
29	3.820287560685268E-5	0.0	0.0	2.135464340671851	0.0
30	3.820287560685268E-5	0.0	0.0	2.231200746942624	0.0
31	3.820287560685268E-5	0.0	0.0	2.319831418350522	0.0
32	3.820287560685268E-5	0.0	0.0	2.3952820976740563	0.0
33	3.820287560685268E-5	0.0	0.0	2.4727957322803604	0.0
34	7.640575121370536E-5	0.0	0.0	2.5557341752228373	0.0
35	1.1460862682055803E-4	0.0	0.0	2.6632752700561277	0.0
36	1.1460862682055803E-4	0.0	0.0	2.7576363728050537	0.0
37	1.1460862682055803E-4	0.0	0.0	2.8497053030175685	0.0
38	1.1460862682055803E-4	0.0	0.0	2.9442192172689223	0.0
39	1.1460862682055803E-4	0.0	0.0	3.0456096491295095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCA	40	6.8212103E-9	45.000004	23
CGACATA	20	7.0349395E-4	45.000004	43
ATCGTAC	40	6.8212103E-9	45.000004	28
AACGCAT	35	1.2127202E-7	45.000004	38
TCGTAAT	35	1.2127202E-7	45.000004	17
ACGCGGT	75	0.0	45.000004	23
TACGCGC	110	0.0	45.0	34
ACGTAAG	100	0.0	45.0	1
TTCGATC	25	3.8921768E-5	45.0	17
TCGAACA	25	3.8921768E-5	45.0	25
TATATCG	30	2.1665692E-6	44.999996	27
CGTCGGA	30	2.1665692E-6	44.999996	19
CGACGGT	250	0.0	44.100002	28
CGGTCTA	250	0.0	43.2	31
TACGGGA	820	0.0	42.80488	4
TCTAGCG	80	0.0	42.187504	1
CGTTATC	135	0.0	41.666664	34
CGTTTTT	8005	0.0	41.37414	1
AACCGAG	60	3.6379788E-12	41.249996	1
TACGCCT	55	6.184564E-11	40.909092	35
>>END_MODULE