FastQCFastQC Report
Thu 26 May 2016
SRR1545556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545556_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1727682
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67380.39000232681708785No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63840.36951244499855873No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23220.13439973328425023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG351.212411E-745.00000412
GCGATTC650.045.0000049
TGCGTCG351.212411E-745.0000041
ATCTACG207.034275E-445.033
GTTTCGA406.8193913E-945.023
CGTTGCC207.034275E-445.021
TAGCGCA207.034275E-445.029
ATAGCCG207.034275E-445.020
CGTCTAG207.034275E-445.032
CCGTCTA253.8916245E-545.031
ATCCGTC207.034275E-445.015
TATTCGA253.8916245E-545.013
ACCCGTG207.034275E-445.031
ACCCGTA253.8916245E-545.019
CGTCCGA502.1827873E-1145.020
TCGGTAA207.034275E-445.010
TAGTCGA207.034275E-445.044
TGCGTAT207.034275E-445.029
TACCGGC207.034275E-445.030
TACCCGT302.16614E-644.99999618