##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545556_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1727682
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329751655686636	31.0	31.0	33.0	30.0	34.0
2	31.681748724591678	31.0	31.0	34.0	30.0	34.0
3	31.783844480639377	31.0	31.0	34.0	30.0	34.0
4	35.4756349837528	37.0	35.0	37.0	33.0	37.0
5	35.324828874758204	37.0	35.0	37.0	33.0	37.0
6	35.396073467223715	37.0	35.0	37.0	33.0	37.0
7	35.853249035412766	37.0	35.0	37.0	35.0	37.0
8	35.88836950318404	37.0	35.0	37.0	35.0	37.0
9	37.59367580376481	39.0	37.0	39.0	35.0	39.0
10	36.91933237713885	39.0	37.0	39.0	32.0	39.0
11	36.52085221701679	39.0	35.0	39.0	32.0	39.0
12	35.486320399240135	37.0	35.0	39.0	31.0	39.0
13	35.0248338525261	37.0	34.0	39.0	30.0	39.0
14	35.98026778076058	38.0	35.0	40.0	30.0	41.0
15	36.29643186651247	38.0	35.0	40.0	31.0	41.0
16	36.51478512828171	38.0	35.0	40.0	32.0	41.0
17	36.48264495433766	38.0	35.0	40.0	32.0	41.0
18	36.43952764455496	37.0	35.0	40.0	31.0	41.0
19	36.36753349285343	37.0	35.0	40.0	31.0	41.0
20	36.20663351241721	37.0	35.0	40.0	31.0	41.0
21	35.99411523648449	36.0	34.0	40.0	31.0	41.0
22	35.92550828219545	36.0	34.0	40.0	30.0	41.0
23	35.90958000372754	36.0	34.0	40.0	30.0	41.0
24	35.85855325227675	36.0	34.0	40.0	30.0	41.0
25	35.762474228474915	36.0	34.0	40.0	30.0	41.0
26	35.65553498849904	36.0	34.0	40.0	30.0	41.0
27	35.60215826755155	36.0	34.0	40.0	30.0	41.0
28	35.6310466856748	36.0	34.0	40.0	30.0	41.0
29	35.6528649369502	36.0	34.0	40.0	30.0	41.0
30	35.597904012428216	36.0	34.0	40.0	30.0	41.0
31	35.41611245588019	36.0	34.0	40.0	29.0	41.0
32	35.23841713926521	36.0	34.0	40.0	29.0	41.0
33	35.06311636053394	36.0	34.0	40.0	28.0	41.0
34	34.888790877024825	35.0	34.0	40.0	27.0	41.0
35	34.78595713794552	35.0	34.0	40.0	27.0	41.0
36	34.57649497997895	35.0	33.0	40.0	25.0	41.0
37	34.51708705653008	35.0	33.0	40.0	25.0	41.0
38	34.54068225518353	35.0	33.0	40.0	26.0	41.0
39	34.499774264013865	35.0	33.0	40.0	25.0	41.0
40	34.34628421202513	35.0	33.0	40.0	24.0	41.0
41	34.33797018201266	35.0	33.0	40.0	24.0	41.0
42	34.261506457785636	35.0	33.0	40.0	24.0	41.0
43	34.187623648333435	35.0	33.0	40.0	24.0	41.0
44	34.14607202019816	35.0	33.0	40.0	24.0	41.0
45	34.10684605153032	35.0	33.0	40.0	24.0	41.0
46	34.074138643569825	35.0	33.0	40.0	24.0	41.0
47	34.038124492817545	35.0	33.0	40.0	24.0	41.0
48	34.04606692666822	35.0	33.0	39.0	24.0	41.0
49	33.993666079753105	35.0	33.0	39.0	24.0	41.0
50	33.8670415041657	35.0	33.0	39.0	24.0	41.0
51	33.692986903839945	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	5.0
14	14.0
15	45.0
16	185.0
17	411.0
18	1000.0
19	1976.0
20	3377.0
21	5167.0
22	7879.0
23	11129.0
24	15587.0
25	21620.0
26	28437.0
27	33880.0
28	37460.0
29	42854.0
30	50818.0
31	62240.0
32	77400.0
33	100808.0
34	177648.0
35	287243.0
36	111573.0
37	133668.0
38	187734.0
39	327256.0
40	263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.80100157320618	23.404538566703824	29.560358908641753	14.234100951448243
2	30.979370046108023	25.747967507909443	29.64613858337356	13.626523862608975
3	29.15305015622088	24.414215115976205	31.424532986973297	15.008201740829621
4	26.462740249652423	26.917858726316535	31.20076495558789	15.418636068443151
5	23.266608091072317	32.94443074593588	29.676699763035096	14.112261399956704
6	20.877279499352312	41.570786753580805	26.649811713035156	10.902122034031727
7	87.33285407847046	4.772695438165125	6.389891195254682	1.5045592881097332
8	89.25519858399868	3.1225653795084978	5.875386789930091	1.7468492465627354
9	85.23125204754116	4.925732860561145	7.3361880253426275	2.506827066555072
10	53.62207860011276	21.684546114389107	14.23381154633781	10.45956373916033
11	47.97966292407978	19.890813239936517	18.541664496128337	13.587859339855369
12	44.14597130721973	20.439120162159472	22.6155044736242	12.7994040569966
13	20.61803040142804	43.353406471792844	21.932624175050734	14.095938951728385
14	14.609690903765854	43.53897302860133	25.893538278456337	15.957797789176482
15	13.614484610015037	23.06037800937904	48.497235023574945	14.82790235703098
16	14.017162880669012	17.97882943736174	48.52426546088922	19.479742221080038
17	14.912466530298978	18.747026362490317	28.873542700566425	37.46696440664428
18	20.1908105774095	23.15397162209249	36.57050313657258	20.08471466392542
19	29.13406518097659	25.27884182390046	24.96454787397218	20.622545121150768
20	32.23926625385921	21.664056232570577	26.181091196180777	19.915586317389426
21	20.707109294418764	29.34388388603921	27.814204234344054	22.13480258519797
22	22.32337895515494	24.271422634489447	24.485640297230624	28.91955811312498
23	18.48638812003598	32.16593099887595	24.5299192791266	24.81776160196147
24	18.2107586928613	23.41356800614928	41.956042836586825	16.419630464402594
25	15.996983241128865	26.27838919430775	37.18027970425113	20.544347860312257
26	15.870397445826256	35.49634712869614	27.138153896376764	21.49510152910084
27	16.658389680508336	36.89023790257698	27.353992227736356	19.09738018917833
28	13.950020895048972	29.500104764649976	38.88047684701236	17.669397493288695
29	14.994541819617268	24.442171649643857	37.71371120379792	22.849575326940954
30	16.98067121148452	33.822891018138755	30.29145409861306	18.90498367176367
31	28.26642865990385	28.525793519872288	24.06739203163545	19.14038578858841
32	30.15242388356191	25.639093305365222	26.45666274233337	17.7518200687395
33	28.430116190363737	28.16033274641977	23.895195990928887	19.51435507228761
34	18.30128461140418	27.972277305661574	28.22301789333917	25.503420189595076
35	19.916743937831153	24.795419527436184	32.33708518118497	22.9507513535477
36	31.0413027397403	26.132876304782943	24.753803072556178	18.072017882920584
37	19.249780920331403	34.93466969037126	28.7516452680528	17.063904121244537
38	19.884677851595374	33.958911420041424	24.95945434402859	21.196956384334616
39	20.44948086511291	32.95438628173471	27.21067881705082	19.385454036101553
40	25.310213337871208	26.159270050854268	25.483566999019498	23.046949612255034
41	16.03095940109349	25.364389974543926	28.630905455980905	29.973745168381683
42	20.808458964091773	25.16435316221388	23.817577540311238	30.209610333383115
43	20.411626676668508	26.362143033266538	26.657394126928452	26.568836163136506
44	18.321600850156454	30.885255504195797	28.937269705883374	21.85587393976438
45	16.889566482720777	40.24484829962922	21.25217487940489	21.613410338245117
46	20.17205712625356	35.62189106560119	25.430258577678067	18.77579323046718
47	20.59105784513585	28.355391790850398	26.970414694370838	24.083135669642907
48	21.807311762234022	24.64533403716656	32.21067302894862	21.336681171650802
49	20.387721814546893	25.19063114624103	33.029400086358486	21.39224695285359
50	19.23779954875955	35.37196081223281	26.261372173814397	19.12886746519325
51	18.293239149334195	34.174576108334755	24.096679828811087	23.435504913519964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1254.0
1	1948.0
2	2642.0
3	7009.0
4	11376.0
5	8600.5
6	5825.0
7	6129.0
8	6433.0
9	6833.0
10	7233.0
11	7636.0
12	8039.0
13	7749.5
14	7460.0
15	7165.0
16	6870.0
17	7096.0
18	7322.0
19	6491.0
20	5660.0
21	6055.5
22	6451.0
23	7462.0
24	8473.0
25	8230.5
26	10119.5
27	12251.0
28	17781.5
29	23312.0
30	26148.5
31	28985.0
32	34287.5
33	39590.0
34	47349.0
35	55108.0
36	57210.5
37	59313.0
38	68978.5
39	78644.0
40	97060.0
41	115476.0
42	145550.0
43	175624.0
44	181014.5
45	186405.0
46	179622.5
47	172840.0
48	160854.0
49	148868.0
50	131248.0
51	113628.0
52	102899.0
53	92170.0
54	82006.0
55	71842.0
56	66086.0
57	60330.0
58	56042.5
59	51755.0
60	46927.0
61	42099.0
62	38311.5
63	34524.0
64	29402.0
65	24280.0
66	20986.5
67	17693.0
68	14303.5
69	10914.0
70	9266.5
71	7619.0
72	6486.5
73	5354.0
74	4027.5
75	2252.5
76	1804.0
77	1515.5
78	1227.0
79	720.0
80	213.0
81	128.5
82	44.0
83	33.5
84	23.0
85	19.0
86	15.0
87	8.0
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1727682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.852118779514385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61973180839401	11.973738327908617
2	7.538004370110551	2.2391067253076087
3	2.6233554456466432	1.168871600388895
4	1.3389338213681434	0.7954401661147504
5	0.78491479808736	0.582882390649601
6	0.5040279586691663	0.4491529866210374
7	0.338337971979935	0.35175250232262034
8	0.24264618949584493	0.28830480222310756
9	0.20153783906102116	0.2693937531870846
>10	2.5724444539745583	9.48358144517652
>50	1.2322635437047447	13.26816741656714
>100	1.9826100060937542	56.12445306216964
>500	0.01805226846403656	1.6658649304561137
>1k	0.002354643712700421	0.574464320862652
>5k	7.84881237566807E-4	0.7648255700446144
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6738	0.39000232681708785	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6384	0.36951244499855873	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2322	0.13439973328425023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02130021612773647	0.0
2	0.0	0.0	0.0	0.07489804257959509	0.0
3	0.0	0.0	0.0	0.11217342080313392	0.0
4	0.0	0.0	0.0	0.19465387727602648	0.0
5	0.0	0.0	0.0	0.3299797069136566	0.0
6	0.0	0.0	0.0	0.4659422277942353	0.0
7	0.0	0.0	0.0	0.5481911601787829	0.0
8	0.0	0.0	0.0	0.7407613206597048	0.0
9	0.0	0.0	0.0	0.8094082128539859	0.0
10	5.7881022086240406E-5	0.0	0.0	0.9281221891528649	0.0
11	5.7881022086240406E-5	0.0	0.0	1.0690624779328604	0.0
12	5.7881022086240406E-5	0.0	0.0	1.1898601710268442	0.0
13	5.7881022086240406E-5	0.0	0.0	1.2362228697179227	0.0
14	5.7881022086240406E-5	0.0	0.0	1.251734983637035	0.0
15	5.7881022086240406E-5	0.0	0.0	1.2748873924715312	0.0
16	5.7881022086240406E-5	0.0	0.0	1.3394247320976893	0.0
17	1.1576204417248081E-4	0.0	0.0	1.4147858228539743	0.0
18	1.1576204417248081E-4	0.0	0.0	1.5251649319724347	0.0
19	1.1576204417248081E-4	0.0	0.0	1.5739007525690492	0.0
20	1.1576204417248081E-4	0.0	0.0	1.624662408938682	0.0
21	1.1576204417248081E-4	0.0	0.0	1.7017599303575541	0.0
22	1.1576204417248081E-4	0.0	0.0	1.7774683072463566	0.0
23	1.736430662587212E-4	0.0	0.0	1.8686887980542715	0.0
24	1.736430662587212E-4	0.0	0.0	1.9364095938951729	0.0
25	1.736430662587212E-4	0.0	0.0	1.9911650407887562	0.0
26	1.736430662587212E-4	0.0	0.0	2.047425394256582	0.0
27	1.736430662587212E-4	0.0	0.0	2.0984185747145596	0.0
28	2.3152408834496162E-4	0.0	0.0	2.1564732398670587	0.0
29	2.3152408834496162E-4	0.0	0.0	2.2202581262060956	0.0
30	2.3152408834496162E-4	0.0	0.0	2.304532894363662	0.0
31	2.3152408834496162E-4	0.0	0.0	2.385971492439002	0.0
32	2.3152408834496162E-4	0.0	0.0	2.4626059656811843	0.0
33	2.3152408834496162E-4	0.0	0.0	2.532121073206759	0.0
34	2.3152408834496162E-4	0.0	0.0	2.611070787332391	0.0
35	2.3152408834496162E-4	0.0	0.0	2.7226075168925763	0.0
36	2.8940511043120206E-4	0.0	0.0	2.8093132879777643	0.0
37	2.8940511043120206E-4	0.0	0.0	2.896887274394246	0.0
38	2.8940511043120206E-4	0.0	0.0	2.983766688545693	0.0
39	2.8940511043120206E-4	0.0	0.0	3.072151009271382	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	35	1.212411E-7	45.000004	12
GCGATTC	65	0.0	45.000004	9
TGCGTCG	35	1.212411E-7	45.000004	1
ATCTACG	20	7.034275E-4	45.0	33
GTTTCGA	40	6.8193913E-9	45.0	23
CGTTGCC	20	7.034275E-4	45.0	21
TAGCGCA	20	7.034275E-4	45.0	29
ATAGCCG	20	7.034275E-4	45.0	20
CGTCTAG	20	7.034275E-4	45.0	32
CCGTCTA	25	3.8916245E-5	45.0	31
ATCCGTC	20	7.034275E-4	45.0	15
TATTCGA	25	3.8916245E-5	45.0	13
ACCCGTG	20	7.034275E-4	45.0	31
ACCCGTA	25	3.8916245E-5	45.0	19
CGTCCGA	50	2.1827873E-11	45.0	20
TCGGTAA	20	7.034275E-4	45.0	10
TAGTCGA	20	7.034275E-4	45.0	44
TGCGTAT	20	7.034275E-4	45.0	29
TACCGGC	20	7.034275E-4	45.0	30
TACCCGT	30	2.16614E-6	44.999996	18
>>END_MODULE