FastQCFastQC Report
Thu 26 May 2016
SRR1545555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545555_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2067154
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68890.3332601247899286No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49600.23994341979359063No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27030.13075948864961198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG551.8189894E-1245.0000041
ATACGAC207.0345955E-445.022
TATACGA207.0345955E-445.021
TCACGAC1850.043.78378325
CGGTCTA1800.043.7531
CGTTAGG2050.042.804882
TTGCGTA750.042.043
CGAATAT3400.041.69117714
CACGACG1950.041.5384626
GTGCGAT650.041.5384630
CTACGAA3500.041.14285711
TAGGGCG5650.040.221245
ATTACGT403.460009E-739.37537
AGTCGTA403.460009E-739.37520
CGGCTAC6550.039.16030525
GCGTTAG1150.039.1304321
TAACGGG5750.038.7391323
CGCGATT356.2507115E-638.57142623
CCGCTCG700.038.57142619
CAAATCG356.2507115E-638.57142627
Produced by FastQC (version 0.11.5)