##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545554_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1530212
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29834624222003	31.0	31.0	33.0	30.0	34.0
2	31.643563767634813	31.0	31.0	34.0	30.0	34.0
3	31.73266972158106	31.0	31.0	34.0	30.0	34.0
4	35.43061222889377	37.0	35.0	37.0	33.0	37.0
5	35.28859073121894	37.0	35.0	37.0	33.0	37.0
6	35.37790842053258	37.0	35.0	37.0	33.0	37.0
7	35.85841373613591	37.0	35.0	37.0	35.0	37.0
8	35.889557786764186	37.0	35.0	37.0	35.0	37.0
9	37.60572652678191	39.0	37.0	39.0	35.0	39.0
10	36.9324838649808	39.0	37.0	39.0	32.0	39.0
11	36.4550493657088	39.0	35.0	39.0	32.0	39.0
12	35.37481734557042	37.0	35.0	39.0	30.0	39.0
13	34.897905649674684	37.0	34.0	39.0	29.0	39.0
14	35.78005727310987	37.0	34.0	40.0	29.0	41.0
15	36.02799677430317	37.0	35.0	40.0	30.0	41.0
16	36.19930767762898	37.0	35.0	40.0	31.0	41.0
17	36.11303074345254	37.0	34.0	40.0	31.0	41.0
18	36.012451215909955	37.0	34.0	40.0	30.0	41.0
19	35.99327348106014	37.0	34.0	40.0	30.0	41.0
20	35.880506753312616	36.0	34.0	40.0	30.0	41.0
21	35.70636290919167	36.0	34.0	40.0	30.0	41.0
22	35.60459988550606	36.0	34.0	40.0	30.0	41.0
23	35.613165365321926	35.0	34.0	40.0	30.0	41.0
24	35.57864792590831	35.0	34.0	40.0	30.0	41.0
25	35.50934184282962	35.0	34.0	40.0	30.0	41.0
26	35.422559749890866	35.0	34.0	40.0	30.0	41.0
27	35.372916301793474	35.0	34.0	40.0	29.0	41.0
28	35.35756940868324	36.0	34.0	40.0	29.0	41.0
29	35.361125778650276	36.0	34.0	40.0	29.0	41.0
30	35.28080226792105	36.0	34.0	40.0	29.0	41.0
31	35.06365523208549	35.0	34.0	40.0	29.0	41.0
32	34.91802965863553	35.0	34.0	40.0	28.0	41.0
33	34.743315958834465	35.0	34.0	40.0	27.0	41.0
34	34.54277381173328	35.0	34.0	40.0	25.0	41.0
35	34.41305649151882	35.0	33.0	40.0	25.0	41.0
36	34.32464717307144	35.0	33.0	40.0	24.0	41.0
37	34.296497478780715	35.0	33.0	40.0	24.0	41.0
38	34.31485375882558	35.0	33.0	40.0	25.0	41.0
39	34.26176634348705	35.0	33.0	40.0	24.0	41.0
40	34.105037079829465	35.0	33.0	40.0	23.0	41.0
41	34.116600183503984	35.0	33.0	40.0	24.0	41.0
42	34.063517342695	35.0	33.0	40.0	24.0	41.0
43	33.98467924705858	35.0	33.0	40.0	24.0	41.0
44	33.940321994599444	35.0	33.0	40.0	23.0	41.0
45	33.90243639443423	35.0	33.0	39.0	23.0	41.0
46	33.899977258053134	35.0	33.0	39.0	23.0	41.0
47	33.855465778597996	35.0	33.0	39.0	23.0	41.0
48	33.85808633052152	35.0	33.0	39.0	23.0	41.0
49	33.833637430630525	35.0	33.0	39.0	24.0	41.0
50	33.70598649076076	35.0	33.0	39.0	24.0	41.0
51	33.525965683186385	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	7.0
14	19.0
15	39.0
16	187.0
17	435.0
18	971.0
19	1885.0
20	3372.0
21	5164.0
22	7578.0
23	10846.0
24	15355.0
25	20885.0
26	26603.0
27	31557.0
28	35815.0
29	40699.0
30	48445.0
31	58778.0
32	72763.0
33	93500.0
34	166557.0
35	246586.0
36	97738.0
37	116010.0
38	162574.0
39	265731.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.06954330511066	24.156652803663807	29.337895664130198	14.435908227095332
2	30.715417210164343	25.932681223255337	29.38605892516854	13.965842641411777
3	28.822346184711662	25.50110703614924	31.773505893301056	13.903040885838042
4	25.73787161517489	27.587353909131547	31.431657835646305	15.24311664004726
5	22.48943283675726	32.07254942452418	30.665293436465014	14.772724302253543
6	20.65818331054782	41.75493330335927	27.303079573287885	10.283803812805022
7	87.81665546996102	4.107404725619719	6.636988861674069	1.4389509427451883
8	89.26253355744171	2.741123452175254	6.276189181629735	1.720153808753297
9	85.7828196354492	4.171513489634116	7.627570558850668	2.418096316066009
10	56.61405086354048	20.42991428638646	13.069430902384768	9.886603947688293
11	50.12115968244923	19.772489040734225	17.906080987471018	12.200270289345529
12	46.62634981296709	18.117228201059724	21.492838900753622	13.763583085219564
13	25.753098263508583	35.99788787435989	23.116666187430237	15.132347674701283
14	19.356010801117755	37.32463214247438	26.81504262154525	16.50431443486262
15	18.72152355359911	21.514012437492323	46.52884698329382	13.23561702561475
16	22.413038193400652	17.429284308318064	43.741912885273415	16.415764613007873
17	21.964538246988	19.1974053268436	27.336277587680662	31.501778838487738
18	25.059076781517852	24.266637563945388	33.36779478921875	17.306490865318008
19	31.30762273462762	26.47554717908368	23.894466910467308	18.32236317582139
20	33.62442589654244	24.87472324096269	24.033336557287488	17.46751430520738
21	25.715913873371793	28.898544776802172	27.313404939969104	18.072136409856935
22	24.42537373906361	24.606721160205254	26.387650861449263	24.580254239281878
23	22.095565843164213	28.651324130251233	26.15539546154389	23.09771456504066
24	25.33943009203953	19.708968430518127	38.4797008519081	16.47190062553424
25	21.73574641945038	20.8815510530567	34.38771882588817	22.994983701604745
26	18.33811262753135	30.1509856150651	28.038206470737386	23.472695286666163
27	17.523258215201555	32.16266765650773	28.748957660768575	21.565116467522145
28	16.33577569643945	28.37606815264813	35.55245939778279	19.73569675312963
29	18.943322885979196	25.466732714159868	34.366479938727444	21.22346446113349
30	23.50595865148097	28.98493803472983	30.016233044833	17.4928702689562
31	30.764364676267082	25.592924379105643	25.758914451069526	17.883796493557753
32	33.35544355945451	24.954320054998917	26.135594283667885	15.554642101878693
33	31.681231097390427	25.986464620588517	24.74689781546609	17.58540646655496
34	20.74379236341108	26.41679714967599	30.72809519203875	22.111315294874174
35	19.60970113944996	26.775505616215266	33.62939252861695	19.985400715717823
36	28.75627690803627	29.809333608676447	25.086066505817495	16.34832297746979
37	21.343382485564092	36.87090416229908	25.719900249115806	16.06581310302102
38	23.71717121549171	34.44934427386532	21.935130557073137	19.898353953569835
39	22.5449153450633	32.99947981064062	24.757092481303246	19.69851236299284
40	25.929871155107918	25.09096778746997	23.922632942363542	25.05652811505857
41	17.047768544489262	25.413341419358886	26.041685727206428	31.497204308945427
42	20.64779259344457	26.24414133466474	23.927011420639754	29.18105465125094
43	21.299728403646032	24.340548891264742	26.482605024663247	27.87711768042598
44	19.058339628757324	27.280533677686492	29.495978335028088	24.1651483585281
45	19.56885712567932	35.25602988344099	23.477073764942375	21.698039225937322
46	22.010871696209414	32.8832867602659	25.028623484850463	20.077218058674223
47	20.530292534629186	27.9578908020588	26.0620097084587	25.449806954853315
48	22.219731645026965	22.674374531110722	32.7006323306836	22.405261493178724
49	22.887482257360418	21.969831631172674	32.7204335085596	22.422252602907307
50	20.29790643387975	29.88461729485849	27.674466021701566	22.14301024956019
51	17.730484403468278	29.954150143901632	27.73197439309063	24.583391059539462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1085.0
1	2242.0
2	3399.0
3	8268.5
4	13138.0
5	9562.5
6	5987.0
7	5981.0
8	5975.0
9	6199.0
10	6423.0
11	6597.5
12	6772.0
13	6559.0
14	6346.0
15	6145.5
16	5945.0
17	5898.0
18	5851.0
19	5372.0
20	4893.0
21	4723.5
22	4554.0
23	5054.5
24	5555.0
25	6313.5
26	8894.5
27	10717.0
28	12873.5
29	15030.0
30	16567.0
31	18104.0
32	21592.0
33	25080.0
34	30436.5
35	35793.0
36	36906.5
37	38020.0
38	45215.5
39	52411.0
40	70636.0
41	88861.0
42	110605.0
43	132349.0
44	133405.5
45	134462.0
46	134521.5
47	134581.0
48	126551.5
49	118522.0
50	113244.0
51	107966.0
52	103572.5
53	99179.0
54	94992.5
55	90806.0
56	91306.0
57	91806.0
58	83843.5
59	75881.0
60	68496.0
61	61111.0
62	52614.0
63	44117.0
64	36507.5
65	28898.0
66	22208.5
67	15519.0
68	12939.0
69	10359.0
70	8576.5
71	6794.0
72	5749.0
73	4704.0
74	3675.0
75	2076.5
76	1507.0
77	1280.5
78	1054.0
79	735.0
80	416.0
81	330.0
82	244.0
83	175.5
84	107.0
85	103.0
86	99.0
87	83.5
88	68.0
89	35.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1530212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.884652155676687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92121220422652	13.494418679283873
2	6.925553285125276	2.338711164098885
3	2.2637951630219533	1.1467018163798723
4	1.1530201325242486	0.7787337546465669
5	0.6699014644482679	0.5655526602893709
6	0.46441298984461554	0.47048710740624855
7	0.3505982266073057	0.41438103718630276
8	0.2793122732771679	0.37728724616770304
9	0.25741494901142314	0.3911725686356217
>10	4.5260414967636535	19.794964599724644
>50	1.8309400997354615	21.892068438184765
>100	1.3531169993598506	37.21951952246197
>500	0.0039005967119050177	0.38100136506433485
>1k	0.0	0.0
>5k	7.801193423810034E-4	0.7350000404698318
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6088	0.3978533693370592	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5072	0.3314573405515053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014573144113364684	0.0
2	0.0	0.0	0.0	0.056201362948401924	0.0
3	0.0	0.0	0.0	0.08711211257002298	0.0
4	0.0	0.0	0.0	0.15827872216398772	0.0
5	0.0	0.0	0.0	0.28884886538597265	0.0
6	0.0	0.0	0.0	0.42686895671972247	0.0
7	0.0	0.0	0.0	0.507707428774575	0.0
8	0.0	0.0	0.0	0.7014714301024956	0.0
9	0.0	0.0	0.0	0.7748599540455833	0.0
10	0.0	0.0	0.0	0.8995485592845959	0.0
11	0.0	0.0	0.0	1.0556053670994607	0.0
12	0.0	0.0	0.0	1.1778106563012183	0.0
13	0.0	0.0	0.0	1.2243401567887326	0.0
14	0.0	0.0	0.0	1.2426382749579796	0.0
15	0.0	0.0	0.0	1.269170546303388	0.0
16	0.0	0.0	0.0	1.3377231390160318	0.0
17	0.0	0.0	0.0	1.415098038703134	0.0
18	0.0	0.0	0.0	1.5341665076473063	0.0
19	0.0	0.0	0.0	1.597164314487143	0.0
20	0.0	0.0	0.0	1.6644752491811592	0.0
21	0.0	0.0	0.0	1.7596908140832774	0.0
22	0.0	0.0	0.0	1.8516388578837442	0.0
23	0.0	0.0	0.0	1.9581600457975759	0.0
24	0.0	0.0	0.0	2.0367112530812723	0.0
25	0.0	0.0	0.0	2.105590597904081	0.0
26	0.0	0.0	0.0	2.1750580965251873	0.0
27	0.0	0.0	0.0	2.2387747580073873	0.0
28	6.535042203302549E-5	0.0	0.0	2.3107909230877812	0.0
29	6.535042203302549E-5	0.0	0.0	2.3853557546274633	0.0
30	6.535042203302549E-5	0.0	0.0	2.479917815309251	0.0
31	6.535042203302549E-5	0.0	0.0	2.5796425593316483	0.0
32	1.3070084406605097E-4	0.0	0.0	2.6726362098846432	0.0
33	1.3070084406605097E-4	0.0	0.0	2.768766680695224	0.0
34	1.3070084406605097E-4	0.0	0.0	2.873458056792131	0.0
35	1.3070084406605097E-4	0.0	0.0	2.998996217517573	0.0
36	1.3070084406605097E-4	0.0	0.0	3.10996123412965	0.0
37	1.9605126609907646E-4	0.0	0.0	3.2287683013856903	0.0
38	2.6140168813210195E-4	0.0	0.0	3.3571165302585526	0.0
39	2.6140168813210195E-4	0.0	0.0	3.505004535319289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.034019E-4	45.000004	8
TACCCGC	20	7.034019E-4	45.000004	24
ACCGGGT	20	7.034019E-4	45.000004	4
CGTATGA	20	7.034019E-4	45.000004	15
CTCCGAT	20	7.034019E-4	45.000004	30
CGACGAA	45	3.8562575E-10	45.000004	29
ACGTTAG	45	3.8562575E-10	45.000004	1
TTAGCGA	20	7.034019E-4	45.000004	26
GCGAAGT	20	7.034019E-4	45.000004	43
GTTTACG	30	2.1659744E-6	45.000004	1
TAAACGC	35	1.2122837E-7	45.000004	30
TAAGTCG	30	2.1659744E-6	45.000004	1
TAGCCGT	45	3.8562575E-10	45.000004	44
GTAATCG	35	1.2122837E-7	45.000004	38
AATGCGT	20	7.034019E-4	45.000004	24
ATATCGA	20	7.034019E-4	45.000004	25
TCAATCG	20	7.034019E-4	45.000004	37
CGCTACG	20	7.034019E-4	45.000004	42
GTCGTAA	40	6.8175723E-9	45.000004	35
GTGTACC	30	2.1659744E-6	45.000004	24
>>END_MODULE