FastQCFastQC Report
Thu 26 May 2016
SRR1545553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545553_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2587979
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92550.35761495746294697No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52040.2010835482049893No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT36500.14103669311072461No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29650.11456816303378041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT302.1665583E-645.00000413
AGTCGAT302.1665583E-645.00000443
TAGCGAT253.8921644E-545.031
CAATTCG253.8921644E-545.01
TATCTCG207.034925E-445.030
CTATTCG207.034925E-445.028
CGCCGAT207.034925E-445.020
GCTCGAT502.1827873E-1145.011
TCGAATA406.8212103E-945.042
TCGGTAA207.034925E-445.038
ATACCCG351.2127202E-745.019
GTATGCG950.042.631581
CGGTCTA3250.042.23076631
ACGTAAG1450.041.8965531
AAACTCG650.041.5384619
CGATATC650.041.5384610
CGTGCTA603.6379788E-1241.25000445
ACCGAAT603.6379788E-1241.25000425
CATACGA8650.041.09826718
ACGCATC556.184564E-1140.90909223