FastQCFastQC Report
Thu 26 May 2016
SRR1545552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545552_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1302235
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC62030.47633491650892507No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48520.37259020069342325No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33310.2557910054636836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGT302.1657252E-645.00000432
ACGTTAG302.1657252E-645.0000041
CGTGACA302.1657252E-645.00000426
CCCGTGA302.1657252E-645.00000424
ACGGCAT302.1657252E-645.00000439
TAATGCG600.045.0000041
GTAACGT302.1657252E-645.0000049
AACACGT551.8189894E-1245.00000441
TAGAGTC207.0336333E-445.029
AACAAGT253.8910934E-545.036
GGTAATC253.8910934E-545.08
CTCGTAG453.8562575E-1045.01
ACGCATA207.0336333E-445.018
ACACCGA207.0336333E-445.011
TAGGTCG207.0336333E-445.01
CGGTTGA253.8910934E-545.040
GGCGTAG207.0336333E-445.01
TTATGAG351.2121018E-745.01
TAGCGCG253.8910934E-545.01
GCGTTAG351.2121018E-745.01