##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545552_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1302235
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3974597518881	31.0	31.0	33.0	30.0	34.0
2	31.745389273057473	31.0	31.0	34.0	30.0	34.0
3	31.834913053327547	31.0	31.0	34.0	30.0	34.0
4	35.5133973514765	37.0	35.0	37.0	33.0	37.0
5	35.38072083763683	37.0	35.0	37.0	33.0	37.0
6	35.46647878455118	37.0	35.0	37.0	33.0	37.0
7	35.90324327022388	37.0	35.0	37.0	35.0	37.0
8	35.94678917399701	37.0	35.0	37.0	35.0	37.0
9	37.69987060707169	39.0	37.0	39.0	35.0	39.0
10	37.06887620130007	39.0	37.0	39.0	33.0	39.0
11	36.6407153854719	39.0	35.0	39.0	32.0	39.0
12	35.43196197306938	37.0	35.0	39.0	31.0	39.0
13	34.86605029046217	37.0	34.0	39.0	29.0	39.0
14	35.78370877760158	37.0	35.0	40.0	30.0	41.0
15	36.146801460565875	37.0	35.0	40.0	31.0	41.0
16	36.40587989111028	37.0	35.0	40.0	32.0	41.0
17	36.3527919307959	37.0	35.0	40.0	32.0	41.0
18	36.32785557138305	36.0	35.0	40.0	31.0	41.0
19	36.27115535982369	36.0	35.0	40.0	31.0	41.0
20	36.08018905957834	36.0	35.0	40.0	31.0	41.0
21	35.86812748851014	36.0	34.0	40.0	31.0	41.0
22	35.78189881242633	35.0	34.0	40.0	30.0	41.0
23	35.80134921884299	35.0	34.0	40.0	31.0	41.0
24	35.743262160823505	35.0	34.0	40.0	31.0	41.0
25	35.670693845580864	35.0	34.0	40.0	30.0	41.0
26	35.53753278018176	35.0	34.0	40.0	30.0	41.0
27	35.47480447077525	35.0	34.0	40.0	30.0	41.0
28	35.515808590615364	36.0	34.0	40.0	30.0	41.0
29	35.55028009537449	36.0	34.0	40.0	30.0	41.0
30	35.49751005002937	36.0	34.0	40.0	30.0	41.0
31	35.28740281132054	35.0	34.0	40.0	30.0	41.0
32	35.085187389372884	35.0	34.0	40.0	29.0	41.0
33	34.93124436065687	35.0	34.0	40.0	29.0	41.0
34	34.78519161288093	35.0	34.0	40.0	27.0	41.0
35	34.646847151243826	35.0	34.0	40.0	27.0	41.0
36	34.40187523757233	35.0	33.0	40.0	25.0	41.0
37	34.33279246833329	35.0	33.0	40.0	25.0	41.0
38	34.38904498803979	35.0	33.0	40.0	27.0	41.0
39	34.35119467684404	35.0	33.0	40.0	26.0	41.0
40	34.157087622433735	35.0	33.0	40.0	24.0	41.0
41	34.18799679013389	35.0	33.0	40.0	24.0	41.0
42	34.10748904767573	35.0	33.0	40.0	25.0	41.0
43	34.02798726804302	35.0	33.0	39.0	24.0	41.0
44	33.96461544959243	35.0	33.0	39.0	24.0	41.0
45	33.89772967244775	35.0	33.0	39.0	24.0	41.0
46	33.88773301285866	35.0	33.0	39.0	24.0	41.0
47	33.84570066078703	35.0	33.0	39.0	24.0	41.0
48	33.90613752510107	35.0	33.0	39.0	24.0	41.0
49	33.879244529597194	35.0	33.0	39.0	24.0	41.0
50	33.72080692040991	35.0	33.0	39.0	24.0	41.0
51	33.53146628680691	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	12.0
15	34.0
16	112.0
17	269.0
18	681.0
19	1267.0
20	2352.0
21	3740.0
22	5768.0
23	8418.0
24	11786.0
25	16504.0
26	21477.0
27	25195.0
28	27961.0
29	32156.0
30	38306.0
31	47270.0
32	59117.0
33	78207.0
34	143328.0
35	242166.0
36	79718.0
37	94898.0
38	132031.0
39	229329.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.666196961377935	22.798266057969567	29.16839126578536	14.367145714867133
2	30.68854699804567	25.7649349003828	29.708769922479426	13.837748179092099
3	28.52611087860486	25.163046608331065	31.500305244445126	14.810537268618951
4	25.374529174841715	28.19299128037566	31.449853521061865	14.982626023720758
5	22.314328826978233	32.652478239334684	30.51000779429212	14.523185139394965
6	20.800316379148157	42.580409833862554	26.505354256336222	10.11391953065307
7	88.3028792806214	4.052340783345556	5.873363870576355	1.7714160654566955
8	89.89514181388152	3.135378791078415	5.527573748209808	1.4419056468302571
9	86.98691096461084	4.024849585520278	6.803687506479246	2.184551943389634
10	56.9117709169236	20.96564752137671	12.321201626434553	9.80137993526514
11	51.37551977945609	17.62938332942979	18.035915176600227	12.959181714513893
12	47.43322057846702	19.018226356993935	21.680956202221566	11.867596862317477
13	19.92904506483085	47.92345467599934	19.68638532983678	12.46111492933303
14	13.236320633372625	48.06137141145799	24.674194749795543	14.028113205373838
15	12.222371538163234	21.28102838581362	53.710428609275596	12.786171466747554
16	13.194008761859418	15.016106923865507	52.22137325444333	19.56851105983175
17	13.092030240317607	16.019228480266616	28.9218919780224	41.966849301393374
18	19.77607728251813	21.30844279258352	37.41121994110126	21.504259983797088
19	29.25217030720262	23.7096990942495	25.540167481291782	21.497963117256102
20	33.51015753685011	19.898251851624323	26.03331963892846	20.55827097259711
21	20.120178001666368	27.784002119433126	28.847327863250488	23.248492015650015
22	21.922694444551098	23.808298809354685	23.80630224191486	30.462704504179356
23	17.608265789200875	30.833144555322196	23.629145277158116	27.929444378318813
24	18.142424370409334	21.22735143810449	44.2562210353738	16.374003156112376
25	15.278732333257821	23.993288461759974	39.1262713719106	21.601707833071604
26	15.976609444531901	35.76627874385191	26.40544909328961	21.85166271832657
27	15.614078872092978	37.007222198758285	27.26205331602975	20.116645613118983
28	12.558332405441414	29.518942433585337	39.83466885777145	18.088056303201803
29	13.170433907858412	23.108501921696163	39.44495425172876	24.276109918716667
30	16.316409864578972	33.172123311076724	32.32573229870185	18.185734525642452
31	29.541442212811052	28.206122550845276	24.127365644449736	18.125069591893936
32	30.69707080519261	25.65066980998053	26.988984323106045	16.66327506172081
33	29.78164463403303	28.103683282971197	23.211325144847127	18.903346938148644
34	18.10648615649249	27.486974317231528	27.434449235353064	26.972090290922914
35	19.056007556239848	24.746378341850743	32.178139890265584	24.019474211643825
36	32.48845254504755	24.428693745752494	25.588545846179837	17.494307863020115
37	19.437889474633995	34.72936912308454	29.048213264118992	16.784528138162464
38	18.910181342077276	34.22769315830092	24.28248357631303	22.579641923308774
39	19.3490422235618	34.394329748470895	27.33876758035224	18.917860447615062
40	24.59786444074994	24.916624111623477	26.21446973856485	24.271041709061727
41	16.033358034456146	22.96497943919492	28.48149527543032	32.52016725091861
42	20.639976655519167	24.612838696548625	24.757820209102043	29.98936443883016
43	21.011875736714185	25.295127223580998	27.18234420054752	26.510652839157295
44	18.056571970496876	30.857410528821603	29.481314816450183	21.60470268423134
45	15.672900820512425	41.109937914431725	21.38300690735543	21.834154357700413
46	20.758158089745706	35.054425660499064	25.44287321412802	18.74454303562721
47	19.64207689088375	29.701935518550798	26.13668039946707	24.51930719109838
48	22.569121548722006	23.5015185431201	31.470049568626244	22.45931033953165
49	20.16970823238509	23.867658295161778	33.74536853947252	22.21726493298061
50	18.504263823349856	36.33284315042984	26.188053615514868	18.974839410705442
51	17.091769150729323	35.001055877011446	24.674885869293945	23.232289102965286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1018.0
1	1480.5
2	1943.0
3	5026.5
4	8110.0
5	6061.0
6	4012.0
7	4435.5
8	4859.0
9	5340.0
10	5821.0
11	5856.5
12	5892.0
13	5684.5
14	5477.0
15	5381.0
16	5285.0
17	5008.5
18	4732.0
19	4236.5
20	3741.0
21	3788.5
22	3836.0
23	3884.5
24	3933.0
25	5472.5
26	7598.5
27	8185.0
28	9282.5
29	10380.0
30	13347.0
31	16314.0
32	20488.5
33	24663.0
34	33198.0
35	41733.0
36	44540.5
37	47348.0
38	52329.0
39	57310.0
40	77446.5
41	97583.0
42	121357.0
43	145131.0
44	148787.0
45	152443.0
46	139934.5
47	127426.0
48	116670.5
49	105915.0
50	96880.0
51	87845.0
52	79539.0
53	71233.0
54	61884.0
55	52535.0
56	48133.0
57	43731.0
58	38451.0
59	33171.0
60	31113.5
61	29056.0
62	27115.0
63	25174.0
64	22533.5
65	19893.0
66	17264.5
67	14636.0
68	12055.0
69	9474.0
70	7727.0
71	5980.0
72	4853.5
73	3727.0
74	3151.0
75	2182.0
76	1789.0
77	1148.0
78	507.0
79	405.0
80	303.0
81	316.5
82	330.0
83	236.0
84	142.0
85	78.5
86	15.0
87	12.0
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1302235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.385129019469503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31893874710694	11.697834256034712
2	7.268567774713562	2.0911857045202584
3	2.333898617678986	1.0072029820102124
4	1.0518305722652854	0.6052287395463428
5	0.5786223501971781	0.41617785805675367
6	0.3940668069810925	0.3401221116428041
7	0.2971127158832258	0.29918033259136323
8	0.25096971697066434	0.2888185406882201
9	0.2064894976856672	0.2673340258836395
>10	2.762008807144444	9.9646253856077
>50	1.3965207140555518	14.771924096417917
>100	2.1232486087159965	55.74563770437434
>500	0.016113700546794812	1.3931812105810208
>1k	0.0010742467031196541	0.6322667542667901
>5k	5.371233515598271E-4	0.47928029777794207
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6203	0.47633491650892507	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4852	0.37259020069342325	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3331	0.2557910054636836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022039032893448572	0.0
2	0.0	0.0	0.0	0.07970911548222863	0.0
3	0.0	0.0	0.0	0.11280606035009041	0.0
4	0.0	0.0	0.0	0.1820715923009288	0.0
5	0.0	0.0	0.0	0.28474123334114043	0.0
6	0.0	0.0	0.0	0.39724012946971937	0.0
7	0.0	0.0	0.0	0.45437267467085435	0.0
8	0.0	0.0	0.0	0.6045759789899673	0.0
9	0.0	0.0	0.0	0.6568706877022964	0.0
10	0.0	0.0	0.0	0.7453339834976022	0.0
11	0.0	0.0	0.0	0.8535325805250205	0.0
12	0.0	0.0	0.0	0.9559718483990984	0.0
13	7.679105537786958E-5	0.0	0.0	0.9944441671434111	0.0
14	7.679105537786958E-5	0.0	0.0	1.008727303443695	0.0
15	7.679105537786958E-5	0.0	0.0	1.029921634727987	0.0
16	7.679105537786958E-5	0.0	0.0	1.08820604575979	0.0
17	7.679105537786958E-5	0.0	0.0	1.1587002345966742	0.0
18	7.679105537786958E-5	0.0	0.0	1.2755762208817918	0.0
19	7.679105537786958E-5	0.0	0.0	1.321343689887002	0.0
20	7.679105537786958E-5	0.0	0.0	1.3697988458304378	0.0
21	7.679105537786958E-5	0.0	0.0	1.4336122128494473	0.0
22	7.679105537786958E-5	0.0	0.0	1.4994989383636594	0.0
23	7.679105537786958E-5	0.0	0.0	1.5966396234166644	0.0
24	7.679105537786958E-5	0.0	0.0	1.6624495578754988	0.0
25	7.679105537786958E-5	0.0	0.0	1.7118262064834688	0.0
26	7.679105537786958E-5	0.0	0.0	1.7596670339838816	0.0
27	7.679105537786958E-5	0.0	0.0	1.811117041087054	0.0
28	7.679105537786958E-5	0.0	0.0	1.8601865254735128	0.0
29	7.679105537786958E-5	0.0	0.0	1.917703025951537	0.0
30	7.679105537786958E-5	0.0	0.0	2.003325052697862	0.0
31	7.679105537786958E-5	0.0	0.0	2.076353346362216	0.0
32	7.679105537786958E-5	0.0	0.0	2.142624027153317	0.0
33	7.679105537786958E-5	0.0	0.0	2.201599557683521	0.0
34	1.5358211075573917E-4	0.0	0.0	2.275088597680142	0.0
35	1.5358211075573917E-4	0.0	0.0	2.3848998068704956	0.0
36	1.5358211075573917E-4	0.0	0.0	2.4621516085806325	0.0
37	1.5358211075573917E-4	0.0	0.0	2.5390962460692577	0.0
38	2.3037316613360877E-4	0.0	0.0	2.608284986964718	0.0
39	2.3037316613360877E-4	0.0	0.0	2.687994102446947	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTGT	30	2.1657252E-6	45.000004	32
ACGTTAG	30	2.1657252E-6	45.000004	1
CGTGACA	30	2.1657252E-6	45.000004	26
CCCGTGA	30	2.1657252E-6	45.000004	24
ACGGCAT	30	2.1657252E-6	45.000004	39
TAATGCG	60	0.0	45.000004	1
GTAACGT	30	2.1657252E-6	45.000004	9
AACACGT	55	1.8189894E-12	45.000004	41
TAGAGTC	20	7.0336333E-4	45.0	29
AACAAGT	25	3.8910934E-5	45.0	36
GGTAATC	25	3.8910934E-5	45.0	8
CTCGTAG	45	3.8562575E-10	45.0	1
ACGCATA	20	7.0336333E-4	45.0	18
ACACCGA	20	7.0336333E-4	45.0	11
TAGGTCG	20	7.0336333E-4	45.0	1
CGGTTGA	25	3.8910934E-5	45.0	40
GGCGTAG	20	7.0336333E-4	45.0	1
TTATGAG	35	1.2121018E-7	45.0	1
TAGCGCG	25	3.8910934E-5	45.0	1
GCGTTAG	35	1.2121018E-7	45.0	1
>>END_MODULE