FastQCFastQC Report
Thu 26 May 2016
SRR1545551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545551_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1832148
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79240.4324978113121865No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72000.39298135303479853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT406.8193913E-945.00000431
ACCGGTT406.8193913E-945.00000432
GCGAATC253.891716E-545.0000049
TTAGCCG406.8193913E-945.00000441
TAGACCG253.891716E-545.0000041
GACGTAT253.891716E-545.00000445
TCGATTG502.1827873E-1145.0000041
TACGGAC253.891716E-545.00000419
CCCGTAG207.034384E-445.00000426
CGCTAAC502.1827873E-1145.00000413
TATTCCG207.034384E-445.00000435
TCGCTCA253.891716E-545.00000424
GCTTAAC406.8193913E-945.00000428
TCGCTAA502.1827873E-1145.00000441
TATTACG406.8193913E-945.0000041
TAGGACG207.034384E-445.0000041
CGATACG207.034384E-445.00000436
AATCGTA207.034384E-445.00000412
TACCGAA207.034384E-445.00000415
AAGTCCG253.891716E-545.0000041