##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545551_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1832148
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.407768368057603	31.0	31.0	33.0	30.0	34.0
2	31.751083973565454	31.0	31.0	34.0	30.0	34.0
3	31.833181598866467	31.0	31.0	34.0	30.0	34.0
4	35.51903066782815	37.0	35.0	37.0	33.0	37.0
5	35.39533651211583	37.0	35.0	37.0	33.0	37.0
6	35.46561194837972	37.0	35.0	37.0	33.0	37.0
7	35.910735377273014	37.0	35.0	37.0	35.0	37.0
8	35.95062625945066	37.0	35.0	37.0	35.0	37.0
9	37.666125225691374	39.0	37.0	39.0	35.0	39.0
10	37.05380569691968	39.0	37.0	39.0	33.0	39.0
11	36.6314877400734	39.0	35.0	39.0	32.0	39.0
12	35.59462499754387	37.0	35.0	39.0	31.0	39.0
13	35.12392557806466	37.0	35.0	39.0	30.0	39.0
14	36.05949410200486	38.0	35.0	40.0	30.0	41.0
15	36.38537115997179	38.0	35.0	40.0	31.0	41.0
16	36.61001349235979	38.0	35.0	40.0	32.0	41.0
17	36.577648748900195	38.0	35.0	40.0	32.0	41.0
18	36.5221035636859	37.0	35.0	40.0	31.0	41.0
19	36.45466523446796	37.0	35.0	40.0	31.0	41.0
20	36.2699623611193	37.0	35.0	40.0	31.0	41.0
21	36.0656213362676	36.0	35.0	40.0	31.0	41.0
22	35.97814150385231	36.0	34.0	40.0	30.0	41.0
23	35.98443193453804	36.0	34.0	40.0	31.0	41.0
24	35.92737158788482	36.0	34.0	40.0	31.0	41.0
25	35.82417686780762	36.0	34.0	40.0	31.0	41.0
26	35.702048087818234	36.0	34.0	40.0	30.0	41.0
27	35.647123485657275	36.0	34.0	40.0	30.0	41.0
28	35.682947010831	36.0	34.0	40.0	30.0	41.0
29	35.72789206985462	36.0	34.0	40.0	30.0	41.0
30	35.65325781541666	36.0	34.0	40.0	30.0	41.0
31	35.4326440877047	36.0	34.0	40.0	30.0	41.0
32	35.238535314832646	35.0	34.0	40.0	29.0	41.0
33	35.05480725356249	35.0	34.0	40.0	29.0	41.0
34	34.897573776790956	35.0	34.0	40.0	27.0	41.0
35	34.71305429474038	35.0	34.0	40.0	27.0	41.0
36	34.520206882850076	35.0	33.0	40.0	25.0	41.0
37	34.44608950805284	35.0	33.0	40.0	25.0	41.0
38	34.473236332436024	35.0	33.0	40.0	26.0	41.0
39	34.44272187618031	35.0	33.0	40.0	26.0	41.0
40	34.23378133207579	35.0	33.0	40.0	24.0	41.0
41	34.23653984285112	35.0	33.0	40.0	24.0	41.0
42	34.18159559162251	35.0	33.0	40.0	24.0	41.0
43	34.08427485115831	35.0	33.0	40.0	24.0	41.0
44	34.0133035104151	35.0	33.0	40.0	24.0	41.0
45	33.965858107532796	35.0	33.0	39.0	24.0	41.0
46	33.95400262424214	35.0	33.0	39.0	24.0	41.0
47	33.906925095570884	35.0	33.0	39.0	24.0	41.0
48	33.902673801461454	35.0	33.0	39.0	24.0	41.0
49	33.853439787615415	35.0	33.0	39.0	24.0	41.0
50	33.70152411268085	35.0	33.0	39.0	24.0	41.0
51	33.518441741606026	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	19.0
15	49.0
16	152.0
17	391.0
18	929.0
19	1735.0
20	3174.0
21	5130.0
22	7668.0
23	11017.0
24	15914.0
25	22198.0
26	29876.0
27	35189.0
28	39041.0
29	44960.0
30	53473.0
31	65172.0
32	82488.0
33	107862.0
34	193545.0
35	311602.0
36	120850.0
37	146865.0
38	205092.0
39	327595.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.11230752100813	23.909804229789298	29.055512982575642	14.922375266626933
2	30.361848496955485	25.911443835323343	30.24924842316232	13.477459244558846
3	28.562212223030016	25.347461012974936	32.179278093254474	13.911048670740572
4	24.79706879575231	27.95751216604772	31.491942790647915	15.753476247552053
5	22.730041459532746	33.015236760349055	30.361357270264193	13.893364509854008
6	21.3904116916319	41.059128411023565	26.70493868399278	10.84552121335176
7	87.71425670851917	4.120245744339432	6.4180950447234615	1.747402502417927
8	89.1441084453876	2.882791128227632	6.353198540729242	1.6199018856555256
9	85.56650445269706	4.187762124020549	7.733545543263973	2.512187880018426
10	55.318020159943416	21.08432288221257	12.563450114292078	11.03420684355194
11	47.81917181363078	18.83341302121881	19.997784021814834	13.349631143335582
12	42.79463231136349	19.416608265271147	24.574161039392013	13.214598383973348
13	20.630320257970425	41.91173420487865	24.119066800280326	13.338878736870601
14	14.738929387800548	43.3802836888723	28.19204562076863	13.688741302558526
15	13.775251780969661	21.91395018306381	50.09786327305436	14.212934762912166
16	14.807919447555548	16.597949510629054	48.75359414195796	19.840536899857437
17	15.77356196115161	16.30556046782247	28.848761126284554	39.07211644474136
18	20.33907741077686	20.710936016086038	37.63593334162961	21.314053231507497
19	30.107720555326317	22.306876955355133	25.661464030198434	21.923938459120116
20	31.746016151533606	22.05187572183033	25.44095782655113	20.76115030008493
21	21.831969906361277	26.878068802301996	29.193766005803024	22.0961952855337
22	22.39240498038368	22.45893890668221	25.230112414499267	29.918543698434842
23	17.605182550754634	29.58303586828138	25.57882878457417	27.23295279638981
24	18.777194855437443	21.972296997840786	41.01704665780275	18.233461488919016
25	17.054299106840716	23.49007831245074	37.893226966380446	21.5623956143281
26	17.22732006366298	33.32170763497272	27.81270945360309	21.638262847761208
27	16.954689249995088	34.85307955470846	28.917150797861307	19.27508039743514
28	14.770859122734626	28.410095690959462	38.44034433899445	18.37870084731146
29	14.601440494981846	23.177658136788075	38.40421188681264	23.81668948141744
30	18.124845809399677	30.93396385008198	32.35011582033766	18.591074520180683
31	29.93491792147796	25.841962548877056	25.150806594227106	19.07231293541788
32	31.0685599634964	24.443112674303606	26.723114071570635	17.765213290629358
33	30.011603866063226	26.524112680853296	24.310972694345654	19.15331075873783
34	20.687029650443087	26.32587542054463	27.286496505740804	25.70059842327148
35	20.387654272471437	25.328630656475347	31.739248139342457	22.544466931710758
36	33.393645054875485	23.3807530832662	25.37999113608726	17.845610725771063
37	20.52017631763373	33.57556267288451	28.44923008403251	17.455030925449254
38	20.655973207404642	32.98592690110188	24.25912098804245	22.09897890345103
39	21.267659599552	31.752784163724762	27.001912509251436	19.977643727471797
40	25.264607444376765	25.255055814268278	25.137052246870883	24.34328449448407
41	17.5305706744215	24.084844674120212	28.366321934690863	30.018262716767424
42	22.251095435521584	23.97126214694446	25.3518274724531	28.42581494508086
43	22.45233463672149	24.02169475391726	27.25685916203276	26.26911144732849
44	18.937225595312167	29.331800706056498	30.098059763730877	21.632913934900454
45	16.52055401637859	38.93599207050959	22.865510864842797	21.67794304826903
46	22.297434486733607	33.118940172955455	26.273587068293608	18.310038272017326
47	21.78262891425802	27.975851295856014	26.135989013987952	24.105530775898018
48	22.806399919657146	24.441038606051478	31.259374242692182	21.493187231599194
49	22.404412743948633	23.430912786521613	33.31717743326412	20.84749703626563
50	20.44561902204407	34.09860993762513	26.12550951124036	19.330261529090446
51	18.500197582291385	33.87035326840408	24.869934088294176	22.759515061010354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1943.0
1	2449.5
2	2956.0
3	7966.0
4	12976.0
5	9782.5
6	6589.0
7	7132.5
8	7676.0
9	8464.5
10	9253.0
11	9359.0
12	9465.0
13	9225.5
14	8986.0
15	8806.0
16	8626.0
17	7993.5
18	7361.0
19	7352.0
20	7343.0
21	7581.0
22	7819.0
23	8051.0
24	8283.0
25	8825.5
26	12268.0
27	15168.0
28	15048.0
29	14928.0
30	19313.0
31	23698.0
32	28213.0
33	32728.0
34	37482.0
35	42236.0
36	46550.0
37	50864.0
38	62394.5
39	73925.0
40	99808.0
41	125691.0
42	154835.0
43	183979.0
44	189795.0
45	195611.0
46	185691.0
47	175771.0
48	158722.0
49	141673.0
50	131438.0
51	121203.0
52	112758.5
53	104314.0
54	94377.5
55	84441.0
56	80566.0
57	76691.0
58	72142.5
59	67594.0
60	61562.0
61	55530.0
62	49047.5
63	42565.0
64	36255.5
65	29946.0
66	25879.0
67	21812.0
68	19315.0
69	16818.0
70	13995.0
71	11172.0
72	8647.5
73	6123.0
74	5284.0
75	3237.0
76	2029.0
77	1684.0
78	1339.0
79	1004.5
80	670.0
81	494.5
82	319.0
83	244.0
84	169.0
85	93.5
86	18.0
87	12.5
88	7.0
89	11.5
90	16.0
91	11.5
92	7.0
93	5.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1832148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.724136320339138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61411573057839	11.869732293606319
2	7.623598179705213	2.245017976989377
3	2.6733106858579223	1.1808657289557412
4	1.3159145224156727	0.7750281925584936
5	0.738573167090428	0.5437425997392039
6	0.47832824735380325	0.4225782191943779
7	0.3453805190240382	0.35598008911495965
8	0.261783884698606	0.3083633283816174
9	0.21672399806020984	0.28719663221947
>10	2.5339224437521035	9.110103675575566
>50	1.229559995446365	13.377939016346405
>100	1.9445332695556976	56.314364453890306
>500	0.022016400480480938	2.116351806788106
>1k	0.001492637320710572	0.2617555430587917
>5k	7.46318660355286E-4	0.8309804435812689
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7924	0.4324978113121865	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7200	0.39298135303479853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022323524082115638	0.0
2	0.0	0.0	0.0	0.11096265148885352	0.0
3	0.0	0.0	0.0	0.16079487028340506	0.0
4	0.0	0.0	0.0	0.247523671668446	0.0
5	5.458074347705535E-5	0.0	0.0	0.4162327497560241	0.0
6	5.458074347705535E-5	0.0	0.0	0.5705325115656595	0.0
7	5.458074347705535E-5	0.0	0.0	0.6547505987507559	0.0
8	5.458074347705535E-5	0.0	0.0	0.8339391795859287	0.0
9	5.458074347705535E-5	0.0	0.0	0.9038025312365595	0.0
10	5.458074347705535E-5	0.0	0.0	1.0495331163202972	0.0
11	5.458074347705535E-5	0.0	0.0	1.2265930481598648	0.0
12	5.458074347705535E-5	0.0	0.0	1.365664782539402	0.0
13	5.458074347705535E-5	0.0	0.0	1.418935588173008	0.0
14	5.458074347705535E-5	0.0	0.0	1.4391850440029954	0.0
15	5.458074347705535E-5	0.0	0.0	1.475208334697852	0.0
16	1.091614869541107E-4	0.0	0.0	1.5582802262699302	0.0
17	1.091614869541107E-4	0.0	0.0	1.66356648043717	0.0
18	1.091614869541107E-4	0.0	0.0	1.8079325469339813	0.0
19	1.091614869541107E-4	0.0	0.0	1.8711916286238885	0.0
20	1.6374223043116604E-4	0.0	0.0	1.9400179461484552	0.0
21	1.6374223043116604E-4	0.0	0.0	2.0298578499116884	0.0
22	1.6374223043116604E-4	0.0	0.0	2.12324550200093	0.0
23	1.6374223043116604E-4	0.0	0.0	2.2328436349028573	0.0
24	1.6374223043116604E-4	0.0	0.0	2.307510091979469	0.0
25	1.6374223043116604E-4	0.0	0.0	2.3691317513650643	0.0
26	2.183229739082214E-4	0.0	0.0	2.435611096920118	0.0
27	2.183229739082214E-4	0.0	0.0	2.5009988276056303	0.0
28	2.7290371738527674E-4	0.0	0.0	2.566113654573757	0.0
29	2.7290371738527674E-4	0.0	0.0	2.644873667411148	0.0
30	2.7290371738527674E-4	0.0	0.0	2.744428943513297	0.0
31	2.7290371738527674E-4	0.0	0.0	2.839017371959034	0.0
32	2.7290371738527674E-4	0.0	0.0	2.9195239685876904	0.0
33	2.7290371738527674E-4	0.0	0.0	2.998993531090283	0.0
34	2.7290371738527674E-4	0.0	0.0	3.0822291648927926	0.0
35	2.7290371738527674E-4	0.0	0.0	3.2055816451509376	0.0
36	2.7290371738527674E-4	0.0	0.0	3.296949809731528	0.0
37	2.7290371738527674E-4	0.0	0.0	3.3929573375076685	0.0
38	2.7290371738527674E-4	0.0	0.0	3.4826880797839475	0.0
39	2.7290371738527674E-4	0.0	0.0	3.5743837288254006	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	40	6.8193913E-9	45.000004	31
ACCGGTT	40	6.8193913E-9	45.000004	32
GCGAATC	25	3.891716E-5	45.000004	9
TTAGCCG	40	6.8193913E-9	45.000004	41
TAGACCG	25	3.891716E-5	45.000004	1
GACGTAT	25	3.891716E-5	45.000004	45
TCGATTG	50	2.1827873E-11	45.000004	1
TACGGAC	25	3.891716E-5	45.000004	19
CCCGTAG	20	7.034384E-4	45.000004	26
CGCTAAC	50	2.1827873E-11	45.000004	13
TATTCCG	20	7.034384E-4	45.000004	35
TCGCTCA	25	3.891716E-5	45.000004	24
GCTTAAC	40	6.8193913E-9	45.000004	28
TCGCTAA	50	2.1827873E-11	45.000004	41
TATTACG	40	6.8193913E-9	45.000004	1
TAGGACG	20	7.034384E-4	45.000004	1
CGATACG	20	7.034384E-4	45.000004	36
AATCGTA	20	7.034384E-4	45.000004	12
TACCGAA	20	7.034384E-4	45.000004	15
AAGTCCG	25	3.891716E-5	45.000004	1
>>END_MODULE